作物学报
作物學報
작물학보
ACTA AGRONOMICA SINICA
2010年
1期
47-52
,共6页
刘显君%王振华%王霞%李庭锋%张林
劉顯君%王振華%王霞%李庭鋒%張林
류현군%왕진화%왕하%리정봉%장림
玉米%重组自交系%脱水速率%SSR标记%数量性状基因位点
玉米%重組自交繫%脫水速率%SSR標記%數量性狀基因位點
옥미%중조자교계%탈수속솔%SSR표기%수량성상기인위점
Maize%Recombination inbred line%Kernel dehydration rate%SSR%QTL
以吉846(脱水快,1.18%d~(-1))和掖3189(脱水慢,0.39%d~(-1))为亲本衍生出的232个重组自交系(F_7)为作图群体,构建了具有101个SSR标记位点的玉米遗传连锁图谱,覆盖玉米基因组1 941.7 cM,标记间平均距离为19.22 cM.通过1年2点试验(双城和哈尔滨,2007年)评价了232个重组自交系籽粒生理成熟后的自然脱水速率.采用WinQTL2.5对该性状数量性状位点(QTL)进行了初步定位和遗传效应分析,结果共检测出9个显著影响玉米籽粒生理成熟后自然脱水速率的QTL,分别位于第2、第3、第4、第5和第6染色体上,加性增效作用均来源于亲本吉846.其中在第2和第6染色体上的2个QTL(qKdr-2-1和qKdr-6-1)在2个环境下均稳定表达,分别位于SSR标记bnlg198~urnc1516之间和phi126~phi077之间,其累积表型贡献率为15.49%.具有较快脱水速率的等位基因均来自吉846.所检测到的QTL将在分子辅助选育具有较快脱水速率的材料中具有较大的应用潜力.
以吉846(脫水快,1.18%d~(-1))和掖3189(脫水慢,0.39%d~(-1))為親本衍生齣的232箇重組自交繫(F_7)為作圖群體,構建瞭具有101箇SSR標記位點的玉米遺傳連鎖圖譜,覆蓋玉米基因組1 941.7 cM,標記間平均距離為19.22 cM.通過1年2點試驗(雙城和哈爾濱,2007年)評價瞭232箇重組自交繫籽粒生理成熟後的自然脫水速率.採用WinQTL2.5對該性狀數量性狀位點(QTL)進行瞭初步定位和遺傳效應分析,結果共檢測齣9箇顯著影響玉米籽粒生理成熟後自然脫水速率的QTL,分彆位于第2、第3、第4、第5和第6染色體上,加性增效作用均來源于親本吉846.其中在第2和第6染色體上的2箇QTL(qKdr-2-1和qKdr-6-1)在2箇環境下均穩定錶達,分彆位于SSR標記bnlg198~urnc1516之間和phi126~phi077之間,其纍積錶型貢獻率為15.49%.具有較快脫水速率的等位基因均來自吉846.所檢測到的QTL將在分子輔助選育具有較快脫水速率的材料中具有較大的應用潛力.
이길846(탈수쾌,1.18%d~(-1))화액3189(탈수만,0.39%d~(-1))위친본연생출적232개중조자교계(F_7)위작도군체,구건료구유101개SSR표기위점적옥미유전련쇄도보,복개옥미기인조1 941.7 cM,표기간평균거리위19.22 cM.통과1년2점시험(쌍성화합이빈,2007년)평개료232개중조자교계자립생리성숙후적자연탈수속솔.채용WinQTL2.5대해성상수량성상위점(QTL)진행료초보정위화유전효응분석,결과공검측출9개현저영향옥미자립생리성숙후자연탈수속솔적QTL,분별위우제2、제3、제4、제5화제6염색체상,가성증효작용균래원우친본길846.기중재제2화제6염색체상적2개QTL(qKdr-2-1화qKdr-6-1)재2개배경하균은정표체,분별위우SSR표기bnlg198~urnc1516지간화phi126~phi077지간,기루적표형공헌솔위15.49%.구유교쾌탈수속솔적등위기인균래자길846.소검측도적QTL장재분자보조선육구유교쾌탈수속솔적재료중구유교대적응용잠력.
The aim of this study was to identify QTL for dehydration rate of maize kernel after physiological maturing using 232 recombinant inbred lines derived from a cross between line Ji 846 (more faster kernel dehydration rate, 1.18% d~(-1)) and line Ye 3189 (more slower kernel dehydration rate, 0.39% d~(-1)). Dehydration rate of maize kernel of this population and two parents were evaluated according to the method of Wang et al. (2001) and Jin et al. (1997) at tested locations Shuangcheng and Harbin in 2007, respectively. A molecular genetic map including 10 linkage groups was constructed, covering 1 941.7 cM with mean distance 19.22 cM using 101 SSR markers. A total of nine QTLs were identified through software WinQTL2.5, which were located on chromosomes 2, 3, 4, 5, and 6, respectively. Additive effect of these QTLs was derived from line Ji846. Of them, two QTLs (qKdr-2-1 located in bnlg198-umc1516 of chromosomes 2, qKdr-6-1 located in phi126-phi077 of chromosomes 6) were repeatedly found in two environments, which explained 15.49% of total phenotypic variation. Since beneficial alleles were from line Ji846, it was concluded that these QTLs would have the greatest potential value for marker assisted selection for high kernel dehydration rate of maize after physiological maturing.