CAJ | 학술논문

目的探索高强度聚焦超声波(HIFU)对体外分离的细粒棘球绦虫原头节的急、慢性杀灭作用.方法实验对象为感染细粒棘球蚴病的羊肝原头节.选择声功率为0(对照组)、25、50、100、200、250 W的超声波辐照离体原头节,每种功率辐照时间分别为5、10、20、30、40、50、60 s,观察HIFU对离体原头节的即刻杀灭作用.以不致即刻杀伤的超声剂量作用原头节后,观察HIFU对原头节的迟发性生长抑制作用.光镜下,根据台盼蓝排斥法染色结果及观察原头节形态变化计算原头节死亡率.结果 HIFU对原头节有明显的急性杀伤作用,在一定的功率和时间范围内有剂量-效应关系,不同功率和辐照时间对原头节死亡率的影响,组间比较差异均有统计学意义(F值分别为5201.59、1865.65,P＜0.05),且功率与辐照时间之间存在交互效应(F=214.50,P＜0.05).随着超声照射剂量增大,其生物学效应越明显,声功率≥200 W的短时照射即可致原头节全部即刻死亡,部分原头节被打碎.以不致原头节即刻杀伤的超声照射后,体外培养2～7 d的原头节死亡率均较对照组明显增高(P＜0.05),随超声剂量的增大抑制原头节生长作用增强,培养第2天开始,50 W×10 s组强于25 W×20 s组(P＜0.05).结论 HIFU能够即刻杀灭原头节并能抑制原头节在体外的生长.
목적 탐색고강도취초초성파(HIFU)대체외분리적세립극구조충원두절적급、만성살멸작용.방법 실험대상위감염세립극구유병적양간원두절.선택성공솔위0(대조조)、25、50、100、200、250 W적초성파복조리체원두절,매충공솔복조시간분별위5、10、20、30、40、50、60 s,관찰HIFU대리체원두절적즉각살멸작용.이불치즉각살상적초성제량작용원두절후,관찰HIFU대원두절적지발성생장억제작용.광경하,근거태반람배척법염색결과급관찰원두절형태변화계산원두절사망솔.결과 HIFU대원두절유명현적급성살상작용,재일정적공솔화시간범위내유제량-효응관계,불동공솔화복조시간대원두절사망솔적영향,조간비교차이균유통계학의의(F치분별위5201.59、1865.65,P＜0.05),차공솔여복조시간지간존재교호효응(F=214.50,P＜0.05).수착초성조사제량증대,기생물학효응월명현,성공솔≥200 W적단시조사즉가치원두절전부즉각사망,부분원두절피타쇄.이불치원두절즉각살상적초성조사후,체외배양2～7 d적원두절사망솔균교대조조명현증고(P＜0.05),수초성제량적증대억제원두절생장작용증강,배양제2천개시,50 W×10 s조강우25 W×20 s조(P＜0.05).결론 HIFU능구즉각살멸원두절병능억제원두절재체외적생장.
Objective To evaluate the acute and delayed killing effect of high intensity focused ultrasound (HIFU) on Echinococcus granulosus(E. granulosus)protoscolices in vitro.Methods E. granulosus protoscolices were treated with different dosage of effective power(0,25,50,100,200,250 W)and time(5,10,20,30,40,50,60 s)of HIFU in vitro to obtain the dosage-effect curves.Then the survival pmtoscolices were incubated,and the mortality of each group was counted daily.The protoscolicidal effects were investigated by trypan blue exclusion assay.Results Compared with the untreated group,the Vitality of E.granulosus protoscolices significantly decreased immediately after treated by HIFU of different dosage(F=5201.59 vs 1865.65,P＜0.05),there were the interaction both different dosage and time(F=214.50,P＜0.05).The protoscolices were broken into pieces by HIFU of 250 W×40 s,whereas the growth of the surviving protoscolices after exposed to HIFU was obvious suppressed.Both the acute killing effect and the delayed inhibitory effect showed a dosage-dependant manner.The inhibitory effect increased along with the increased dosage of HIFU(P＜0.05).The inhibitory effect in 50 W×10 s group was stronger than 25 W×20 s group(P＜0.05).The mortality was increased in parallel with the increase of HIFU dosage.Conclusions HIFU show an effective immediately killing effect,as well as a growth-inhibiting effect on the E.granulosus protoscolices in vitro.