中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2008年
7期
433-435
,共3页
陶小华%董玉娥%潘卫利%孟群%程浩
陶小華%董玉娥%潘衛利%孟群%程浩
도소화%동옥아%반위리%맹군%정호
尖锐湿疣%乳头状瘤病毒,人%蛋白质酪氨酸磷酸酶
尖銳濕疣%乳頭狀瘤病毒,人%蛋白質酪氨痠燐痠酶
첨예습우%유두상류병독,인%단백질락안산린산매
Condylomata acuminata%Papillomavirus,human%Protein-tyrosine-phosphatase
目的 探讨尖锐湿疣(CA)患者皮肤损伤中Src同源序列酪氨酸磷酸酶-1(SHP-1)和Src同源序列酪氨酸磷酸酶-2(SHP-2)的不同表达及意义.方法 应用原位杂交技术诊断人类乳头瘤病毒(HPV)6/11相关CA,采用SP免疫组织化学染色技术检测40例HPV6/11阳性CA患者皮肤损伤、20例健康对照皮肤(包皮)中SHP-1和SHP-2的表达及分布.计数资料采用X2检验.结果 CA患者皮肤损伤中SHP-1和SHP-2表达阳性细胞主要位于棘细胞层,棕黄着色,阳性反应定位胞质;而健康对照包皮少数阳性着色主要定位于基底层细胞的胞质,呈浅黄着色.CA患者皮肤损伤和健康对照中SHP-1阳性率分别为80%和35%(X2=11.87,P<0.01);SHP-2表达阳性率分别为85%和30%(X2=18.15,P<0.01).CA患者中SHP-1和SHP-2的表达差异无统计学意义(rs=1.0,P>0.05).结论 CA中SHP-1和SHP-2在HPV6/11感染角质形成细胞过度增殖中起调控作用.
目的 探討尖銳濕疣(CA)患者皮膚損傷中Src同源序列酪氨痠燐痠酶-1(SHP-1)和Src同源序列酪氨痠燐痠酶-2(SHP-2)的不同錶達及意義.方法 應用原位雜交技術診斷人類乳頭瘤病毒(HPV)6/11相關CA,採用SP免疫組織化學染色技術檢測40例HPV6/11暘性CA患者皮膚損傷、20例健康對照皮膚(包皮)中SHP-1和SHP-2的錶達及分佈.計數資料採用X2檢驗.結果 CA患者皮膚損傷中SHP-1和SHP-2錶達暘性細胞主要位于棘細胞層,棕黃著色,暘性反應定位胞質;而健康對照包皮少數暘性著色主要定位于基底層細胞的胞質,呈淺黃著色.CA患者皮膚損傷和健康對照中SHP-1暘性率分彆為80%和35%(X2=11.87,P<0.01);SHP-2錶達暘性率分彆為85%和30%(X2=18.15,P<0.01).CA患者中SHP-1和SHP-2的錶達差異無統計學意義(rs=1.0,P>0.05).結論 CA中SHP-1和SHP-2在HPV6/11感染角質形成細胞過度增殖中起調控作用.
목적 탐토첨예습우(CA)환자피부손상중Src동원서렬락안산린산매-1(SHP-1)화Src동원서렬락안산린산매-2(SHP-2)적불동표체급의의.방법 응용원위잡교기술진단인류유두류병독(HPV)6/11상관CA,채용SP면역조직화학염색기술검측40례HPV6/11양성CA환자피부손상、20례건강대조피부(포피)중SHP-1화SHP-2적표체급분포.계수자료채용X2검험.결과 CA환자피부손상중SHP-1화SHP-2표체양성세포주요위우극세포층,종황착색,양성반응정위포질;이건강대조포피소수양성착색주요정위우기저층세포적포질,정천황착색.CA환자피부손상화건강대조중SHP-1양성솔분별위80%화35%(X2=11.87,P<0.01);SHP-2표체양성솔분별위85%화30%(X2=18.15,P<0.01).CA환자중SHP-1화SHP-2적표체차이무통계학의의(rs=1.0,P>0.05).결론 CA중SHP-1화SHP-2재HPV6/11감염각질형성세포과도증식중기조공작용.
Objective To investigate the different expression pattern of Src-homology2 domain phosphatase (SHP)-1 and SHP-2 in human papiilomavirus (HPV)6/11 infected condyloma acuminatum (CA) and the significance of the difference. Methods HPV6/11 related CA cases were diagnosed by in situ hybridization. The expression and distribution of SHP-1 and SHP-2 were examined by SP immunohistochemistry technique in skin samples from 40 HPV 6/11 positive CA cases and 20 healthy control (foreskins). Results The positive rates of SHP-1 and SHP-2 were 80% and 85% respectively in CA, which were significantly higher than those in healthy control cases (only 35% and 30%, respectively, X2=11.87,P<0.01; X2 =18. 15,P<0. 01) . The SHP-1 and/or SHP-2 positive cells in CA skin lesions were mainly distributed in prickle layer, showing as brown yellow, with the positive staining located in cytoplasm. Contrastively, the SHP-1 and/or SHP-2 positive cells in healthy controls were rare and mainly distributed in basal layer, showing as pale yellow with the positive staining located in cytoplasm. There was no significant correlation between the expression of SHP-1 and SHP-2 in CA( rs = 1.0, P>0.05 ). Conclusion The expressions of SHP-1 and SHP-2 increase in HPV6/11 positive CA, which suggest that with the infection of HPV6/11, SHP-1 and SHP-2 may play a regulatory role in the proliferation of keratinocytes.