中华超声影像学杂志
中華超聲影像學雜誌
중화초성영상학잡지
CHINESE JOURNAL OF ULTRASONOGRAPHY
2011年
5期
441-444
,共4页
李陶%刘政%刘观成%王翔
李陶%劉政%劉觀成%王翔
리도%류정%류관성%왕상
超声疗法%微气泡%前列腺%血-前列腺屏障
超聲療法%微氣泡%前列腺%血-前列腺屏障
초성요법%미기포%전렬선%혈-전렬선병장
Ultrasonic therapy%Microbubbles%Prostate%Blood-prostate barrier
目的 探讨超声联合造影剂微泡的声致空隙效应开放血-前列腺屏障时对前列腺的损伤作用.方法 15只健康8月龄性成熟新西兰兔,随机分为单纯微泡(MB)组、单纯超声(US)组、超声联合微泡(US+MB)组,超声波直接辐射前列腺,光镜、电镜、细胞凋亡指数(AI)观察前列腺组织损伤的情况.结果 MB组与US组前列腺组织在光镜下均未见明显异常,胞浆、细胞核染色均匀,腺体上皮组织排列整齐、完整,腺管腔内未见明显改变;US+MB组腺体上皮组织细胞排列紊乱,腺管腔内可见大量嗜伊红染的液体.透射电镜下MB组与US组前列腺组织微血管内皮细胞排列整齐,细胞间紧密连接形态正常;US+MB组可见线粒体肿胀,微血管内皮细胞间隙增宽,细胞间桥连接断裂,红细胞漏出等改变.US+MB组AI明显高于MB组与US组(P<0.01),而US组则明显高于MB组(P<0.01).结论 利用超声联合造影剂微泡的声致孔隙效应开放血-前列腺屏障的同时可造成前列腺组织损伤.
目的 探討超聲聯閤造影劑微泡的聲緻空隙效應開放血-前列腺屏障時對前列腺的損傷作用.方法 15隻健康8月齡性成熟新西蘭兔,隨機分為單純微泡(MB)組、單純超聲(US)組、超聲聯閤微泡(US+MB)組,超聲波直接輻射前列腺,光鏡、電鏡、細胞凋亡指數(AI)觀察前列腺組織損傷的情況.結果 MB組與US組前列腺組織在光鏡下均未見明顯異常,胞漿、細胞覈染色均勻,腺體上皮組織排列整齊、完整,腺管腔內未見明顯改變;US+MB組腺體上皮組織細胞排列紊亂,腺管腔內可見大量嗜伊紅染的液體.透射電鏡下MB組與US組前列腺組織微血管內皮細胞排列整齊,細胞間緊密連接形態正常;US+MB組可見線粒體腫脹,微血管內皮細胞間隙增寬,細胞間橋連接斷裂,紅細胞漏齣等改變.US+MB組AI明顯高于MB組與US組(P<0.01),而US組則明顯高于MB組(P<0.01).結論 利用超聲聯閤造影劑微泡的聲緻孔隙效應開放血-前列腺屏障的同時可造成前列腺組織損傷.
목적 탐토초성연합조영제미포적성치공극효응개방혈-전렬선병장시대전렬선적손상작용.방법 15지건강8월령성성숙신서란토,수궤분위단순미포(MB)조、단순초성(US)조、초성연합미포(US+MB)조,초성파직접복사전렬선,광경、전경、세포조망지수(AI)관찰전렬선조직손상적정황.결과 MB조여US조전렬선조직재광경하균미견명현이상,포장、세포핵염색균균,선체상피조직배렬정제、완정,선관강내미견명현개변;US+MB조선체상피조직세포배렬문란,선관강내가견대량기이홍염적액체.투사전경하MB조여US조전렬선조직미혈관내피세포배렬정제,세포간긴밀련접형태정상;US+MB조가견선립체종창,미혈관내피세포간극증관,세포간교련접단렬,홍세포루출등개변.US+MB조AI명현고우MB조여US조(P<0.01),이US조칙명현고우MB조(P<0.01).결론 이용초성연합조영제미포적성치공극효응개방혈-전렬선병장적동시가조성전렬선조직손상.
Objective To explore the damage effect of sonoporation on the prostate of rabbit,while opening up the blood-prostate barrier by microbubble mediated sonoporation.Methods Fifteen male New Zealand white rabbits were randomly divided into 3 groups:ultrasound (US) group,microbubble (MB) group,ultrasound and microbubble (US+MB) group.Ultrasound was insonated directly on the prostate.Optical microscope,electron microscope and apoptosis index (AI) with TUNEL method were applied to trace the changes of the prostate of rabbit under different conditions.Results There was no significant change in prostatic tissues of group US and MB under the optical microscope.Cytoplasm and nucleoli were stained equally,cells of glandular epithelium were intact and formed orderly.Glandular cavities in these two groups were change very slightly.Glandular epithelium cells of Group US+MB were organized optical under the optical microscope,and there was a mass of eosinophilic liquid in the glandular cavities.Vascular endothelial cell were intact and formed orderly and swollen mitochondria were observed under the electron microscope in MB group and US group.Swollen mitochondria,tight junctions among gland cells were opened,and infiltrated erythrocyte could be found under the eletron microscope in US+MB.AI of group US+MB was markedly higher than that of group US and group MB (P<0.01),and AI of group US was higher than that of group MB (P<0.01).Conclusions Microbubble mediated sonoporation causes damage in the prostate tissue of rabbit,while opening up the blood-prostate barrier with an increased permeability of the prostate.
