中华皮肤科杂志
中華皮膚科雜誌
중화피부과잡지
Chinese Journal of Dermatology
2010年
3期
160-163
,共4页
蔓小红%张晓艳%应航宇%唐娟%张立新%尤立平
蔓小紅%張曉豔%應航宇%唐娟%張立新%尤立平
만소홍%장효염%응항우%당연%장립신%우립평
银屑病%细胞外信号调节MAP激酶类%MAP激酶激酶激酶类%NF-κB
銀屑病%細胞外信號調節MAP激酶類%MAP激酶激酶激酶類%NF-κB
은설병%세포외신호조절MAP격매류%MAP격매격매격매류%NF-κB
Psoriasis%Extracellular signal-regulated MAP kinases%MAP kinase kinase kinases%NF-kappa B
目的 探讨MAPK信号转导通路中磷酸化MEK和ERK及核因子(NF)-κB在寻常性银屑病发病中的作用.方法 采用免疫组化和western印迹法检测30例寻常性银屑病皮损及15例正常人表皮巾磷酸化MEK、ERK和NF-κB的表达,并利用计算机图像采集与分析系统对免疫组化结果进行平均吸光度(A值)测定,并对免疫印迹测定结果进行灰度扫描、统计学处理.结果 免疫组化检测显示,寻常性银屑病皮损中磷酸化MEK、ERK和NF-κB的表达(A值分别为0.36±0.03、0.36±0.04和0.26±0.04)明显高于正常人皮肤(A值分别为0.22±0.02、0.18±0.03和0.16±0.03),两组比较,P值均<0.01.western印迹结果(两组比较,P值均<0.01)与免疫组化检测一致.结论 寻常性银屑病皮损中磷酸化MEK、ERK和NF-κB表达水平均增高,MAPK信号通路中上下游分子的异常激活可能参与了银屑病的发病.
目的 探討MAPK信號轉導通路中燐痠化MEK和ERK及覈因子(NF)-κB在尋常性銀屑病髮病中的作用.方法 採用免疫組化和western印跡法檢測30例尋常性銀屑病皮損及15例正常人錶皮巾燐痠化MEK、ERK和NF-κB的錶達,併利用計算機圖像採集與分析繫統對免疫組化結果進行平均吸光度(A值)測定,併對免疫印跡測定結果進行灰度掃描、統計學處理.結果 免疫組化檢測顯示,尋常性銀屑病皮損中燐痠化MEK、ERK和NF-κB的錶達(A值分彆為0.36±0.03、0.36±0.04和0.26±0.04)明顯高于正常人皮膚(A值分彆為0.22±0.02、0.18±0.03和0.16±0.03),兩組比較,P值均<0.01.western印跡結果(兩組比較,P值均<0.01)與免疫組化檢測一緻.結論 尋常性銀屑病皮損中燐痠化MEK、ERK和NF-κB錶達水平均增高,MAPK信號通路中上下遊分子的異常激活可能參與瞭銀屑病的髮病.
목적 탐토MAPK신호전도통로중린산화MEK화ERK급핵인자(NF)-κB재심상성은설병발병중적작용.방법 채용면역조화화western인적법검측30례심상성은설병피손급15례정상인표피건린산화MEK、ERK화NF-κB적표체,병이용계산궤도상채집여분석계통대면역조화결과진행평균흡광도(A치)측정,병대면역인적측정결과진행회도소묘、통계학처리.결과 면역조화검측현시,심상성은설병피손중린산화MEK、ERK화NF-κB적표체(A치분별위0.36±0.03、0.36±0.04화0.26±0.04)명현고우정상인피부(A치분별위0.22±0.02、0.18±0.03화0.16±0.03),량조비교,P치균<0.01.western인적결과(량조비교,P치균<0.01)여면역조화검측일치.결론 심상성은설병피손중린산화MEK、ERK화NF-κB표체수평균증고,MAPK신호통로중상하유분자적이상격활가능삼여료은설병적발병.
Objective To investigate the role of phosphorylated extracellular signal-regulated kinase (p-ERK), mitogen-activated protein kinase (MAPK)/ERK kinase (MEK), and nuclear factor-κB (NF-κB) in the pathogenesis of psoriasis vulgaris. Methods Immunohistochemistry and Western blot were used to detect the expression of p-MEK, p-ERK and p-NF-KB in tissue samples from 30 patients with psoriasis vulgaris and 15 normal human controls. The average optical density of immunostaining and relative grey scale of immuno-bloting were calculated. Results The average optical density of immunostaining for p-MEK, p-ERK and p-NF-KB was 0.36 ± 0.03, 0.36 ± 0.04 and 0.26 ± 0.04, respectively in lesion samples of psoriasis, significantly higher than that in normal control tissue (0.22 ± 0.02, 0.18 ± 0.03 and 0.16 ± 0.03, all P < 0.01). A significant increase was also observed in the relative grey scale of p-MEK, p-ERK and p-NF-κB in psoriatic lesions compared with the normal controls (1.41 ± 0.14 vs 0.54 ± 0.10, 2.35 ± 0.34 vs 1.86 ± 0.12, 1.07 ± 0.15 vs 0.87 ± 0.08, all P < 0.01). Conclusions The expressions of p-MEK, p-ERK and p-NF-κB are enhanced in lesions of psoriasis vulgaris, and the abnormal activation of upstream and downstream molecules in the MAPK signaling pathways might be involved in the pathogenesis of psoriasis.
