中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2011年
12期
1081-1086
,共6页
徐光%浦江%褚少朋%王旭东%申娴娟%吴信华%张霞%孙长江%鞠少卿
徐光%浦江%褚少朋%王旭東%申嫻娟%吳信華%張霞%孫長江%鞠少卿
서광%포강%저소붕%왕욱동%신한연%오신화%장하%손장강%국소경
多发性骨髓瘤(MM)%B淋巴细胞刺激因子(BLyS)%MAPK信号通路
多髮性骨髓瘤(MM)%B淋巴細胞刺激因子(BLyS)%MAPK信號通路
다발성골수류(MM)%B림파세포자격인자(BLyS)%MAPK신호통로
Multiple myeloma%B-lymphocyte stimulator (BLyS)%MAPK signal pathway
目的 研究多发性骨髓瘤(MM)细胞中丝裂原活化蛋白激酶(MAPK)信号通路的表达及活化情况,探讨MAPK信号通路对MM细胞B淋巴细胞刺激因子(BLyS)表达变化的影响及对MM细胞增殖与存活的影响,并初步探讨MAPK信号通路在IFN-γ(MM重要的促生长因子)上调MM细胞BLyS表达过程中的作用.方法 应用Western blot方法检测MM细胞中蛋白ERK、p-ERK、JNK、p-JNK、p38及p-p38的表达情况；应用RT-PCR及Western blot检测MAPK信号通路对BLyS表达的影响；应用WST-1法检测靶向JNK的MAPK信号通路抑制剂SP600125对MM细胞增殖与存活的影响.结果 MM细胞株中,除了ERK、JNK及p38的表达外,还有活化蛋白p-JNK的表达；靶向JNK的MAPK信号通路抑制剂SP600125可下调MM细胞BLyS的表达,其激动剂茴香霉素(anisomycin)可上调BLyS的表达；IFN-γ可上调MM细胞BLyS的表达,SP600125可部分抵消IFN-γ对BLyS的上调作用；SP600125可抑制MM细胞的增殖与存活.结论 MM细胞中有JNK/SAPK信号通路的活化；JNK/SAPK信号通路的活化程度与BLyS的表达高低呈正相关；JNK/SAPK信号通路在IFN-γ上调MM细胞BLyS表达过程中发挥重要作用.
目的 研究多髮性骨髓瘤(MM)細胞中絲裂原活化蛋白激酶(MAPK)信號通路的錶達及活化情況,探討MAPK信號通路對MM細胞B淋巴細胞刺激因子(BLyS)錶達變化的影響及對MM細胞增殖與存活的影響,併初步探討MAPK信號通路在IFN-γ(MM重要的促生長因子)上調MM細胞BLyS錶達過程中的作用.方法 應用Western blot方法檢測MM細胞中蛋白ERK、p-ERK、JNK、p-JNK、p38及p-p38的錶達情況；應用RT-PCR及Western blot檢測MAPK信號通路對BLyS錶達的影響；應用WST-1法檢測靶嚮JNK的MAPK信號通路抑製劑SP600125對MM細胞增殖與存活的影響.結果 MM細胞株中,除瞭ERK、JNK及p38的錶達外,還有活化蛋白p-JNK的錶達；靶嚮JNK的MAPK信號通路抑製劑SP600125可下調MM細胞BLyS的錶達,其激動劑茴香黴素(anisomycin)可上調BLyS的錶達；IFN-γ可上調MM細胞BLyS的錶達,SP600125可部分牴消IFN-γ對BLyS的上調作用；SP600125可抑製MM細胞的增殖與存活.結論 MM細胞中有JNK/SAPK信號通路的活化；JNK/SAPK信號通路的活化程度與BLyS的錶達高低呈正相關；JNK/SAPK信號通路在IFN-γ上調MM細胞BLyS錶達過程中髮揮重要作用.
목적 연구다발성골수류(MM)세포중사렬원활화단백격매(MAPK)신호통로적표체급활화정황,탐토MAPK신호통로대MM세포B림파세포자격인자(BLyS)표체변화적영향급대MM세포증식여존활적영향,병초보탐토MAPK신호통로재IFN-γ(MM중요적촉생장인자)상조MM세포BLyS표체과정중적작용.방법 응용Western blot방법검측MM세포중단백ERK、p-ERK、JNK、p-JNK、p38급p-p38적표체정황；응용RT-PCR급Western blot검측MAPK신호통로대BLyS표체적영향；응용WST-1법검측파향JNK적MAPK신호통로억제제SP600125대MM세포증식여존활적영향.결과 MM세포주중,제료ERK、JNK급p38적표체외,환유활화단백p-JNK적표체；파향JNK적MAPK신호통로억제제SP600125가하조MM세포BLyS적표체,기격동제회향매소(anisomycin)가상조BLyS적표체；IFN-γ가상조MM세포BLyS적표체,SP600125가부분저소IFN-γ대BLyS적상조작용；SP600125가억제MM세포적증식여존활.결론 MM세포중유JNK/SAPK신호통로적활화；JNK/SAPK신호통로적활화정도여BLyS적표체고저정정상관；JNK/SAPK신호통로재IFN-γ상조MM세포BLyS표체과정중발휘중요작용.
Objective To investigate the activation of MAPK signal pathway in multiple myeloma and the regulation of BLyS expression levels through MAPK signal pathway; preliminarily study the role of MAPK signal pathway in the up-regulation of BLyS expression levels induced by IFN-γ.Methods Activated MAPK pathway were detected by Western blot,while the expression of BLyS were detected with RT-PCR and Western blot,and Western blot investigated the effect of MAPK pathway on BLyS expression levels induced by IFN-γ.Results In addition to the expression of ERK,JNK,p38,p-JNK was also expressed in MM cell lines,the MAPK pathway inhibitor targeting JNK SP600125 can down-regulate the expression of BLyS,and its activator anisomycin can up-regulate the expression of BLyS.SP600125 restrained the proliferation and survival of MM cells.Conclusion JNK/SAPK pathway was activated in MM cells; The activated degree of JNK/SAPK pathway and the expression level of BLyS was positively correlated.JNK/SAPK pathway play an important role in the up-regulation of BLyS expression levels induced by IFN-γ.
