CAJ | 학술논문

目的筛选前哨淋巴结(SLN)中与结直肠癌早期转移相关的蛋白质.方法术中取43名结直肠癌早期患者的SLN及对应的正常淋巴结(NLN).提取SLN和NLN两组总蛋白后行双向凝胶电泳和质谱法对差异蛋白进行筛选和鉴定.用蛋白质印迹法和免疫组织化学法对其中的转移相关蛋白行进一步研究.结果两组间检测出40种差异表达的蛋白质,SLN中表达升高且与转移相关的蛋白分别为核不均一核糖核蛋白A1( hnRNP A1)、埃兹蛋白(Fzrin)、微管蛋白β-2C (tubulin β-2C)和膜联蛋白A1(Annexin A1).蛋白定量结果显示两组间4种蛋白表达差异有统计学意义(P＜0.05).结论结直肠癌SLN与NLN蛋白表达存在差异.筛选出的4种转移相关蛋白可能成为结直肠癌早期淋巴转移的标志物.
목적 사선전초림파결(SLN)중여결직장암조기전이상관적단백질.방법 술중취43명결직장암조기환자적SLN급대응적정상림파결(NLN).제취SLN화NLN량조총단백후행쌍향응효전영화질보법대차이단백진행사선화감정.용단백질인적법화면역조직화학법대기중적전이상관단백행진일보연구.결과 량조간검측출40충차이표체적단백질,SLN중표체승고차여전이상관적단백분별위핵불균일핵당핵단백A1( hnRNP A1)、애자단백(Fzrin)、미관단백β-2C (tubulin β-2C)화막련단백A1(Annexin A1).단백정량결과현시량조간4충단백표체차이유통계학의의(P＜0.05).결론 결직장암SLN여NLN단백표체존재차이.사선출적4충전이상관단백가능성위결직장암조기림파전이적표지물.
Objective To investigate the early metastasis-associated proteins in sentinel lymph node (SLN) of colorectal cancer.Methods SLN and normal lymph nodes (NLN) were collected from forty-three cases.Hydrophobic protein samples were extracted from individual-matched NLN and SLN of colorectal cancer.Differentially expressed protein spots were detected by two-dimensional electrophoresis and image analysis,and subsequently identified by matrix assisted laser desorption/ionization-time of flight mass spectrometry-mass spectrometry (MALDI-TOF-MS).Metastasis-concerned proteins were selected for further study by western blotting and immunohistochemistry staining.Results Forty proteins were differentially expressed between NLN and SLN,and 4 metastasis-concerned proteins highly expressed in SLN were identified to be hnRNP A1,Ezrin,tubulin β-2C and Annexin A1.Significant difference for these 4 proteins between NLN and SLN was confirmed by western blotting and immunohistochemistry staining.Conclusion Different proteins ware expressed between NLN and SLN.Increased expression of hnRNP A1,Ezrin,tubulin β-2C and Annexin A1 in SLN suggests a significantly elevated early colorectal cancer metastasis