CAJ | 학술논문

目的探讨非小细胞肺癌(NSCLC)组织中肝细胞核因子3β(FoxA 2)的表达情况,及与临床病理因素的关系.方法分别采用免疫组织化学法和反转录聚合酶链反应,检测80例NSCLC组织及癌旁正常肺组织中FoxA 2蛋白及mRNA的表达,并分析其与患者性别、临床病理类型、吸烟、临床分期、淋巴结转移的关系.结果 FoxA 2蛋白和mRNA在腺癌、鳞状细胞癌、腺鳞癌组织中的表达均低于癌旁正常肺组织(蛋白表达比较,t值分别为187.51、186.43、178.24；mRNA表达比较,t值分别为236.70、260.13、126.27,均P＜0.05),不同病理类型间FoxA 2蛋白和mRNA的表达差异无统计学意义(F值分别为1.86、1.69,P＞ 0.05),在低分化组、Ⅲ期及有淋巴结转移患者的肺组织中的表达显著低于相应的高分化组、Ⅰ～Ⅱ期及无淋巴结转移的患者(蛋白比较t值分别为4.31、3.92、3.41；mRNA比较t值分别为5.44、3.28、5.07,均P＜0.05).结论 FoxA 2可能对人类NSCLC的形成起抑制作用,并参与了肿瘤的分化、淋巴结转移过程,且与肺癌的临床分期相关.
목적 탐토비소세포폐암(NSCLC)조직중간세포핵인자3β(FoxA 2)적표체정황,급여림상병리인소적관계.방법 분별채용면역조직화학법화반전록취합매련반응,검측80례NSCLC조직급암방정상폐조직중FoxA 2단백급mRNA적표체,병분석기여환자성별、림상병리류형、흡연、림상분기、림파결전이적관계.결과 FoxA 2단백화mRNA재선암、린상세포암、선린암조직중적표체균저우암방정상폐조직(단백표체비교,t치분별위187.51、186.43、178.24；mRNA표체비교,t치분별위236.70、260.13、126.27,균P＜0.05),불동병리류형간FoxA 2단백화mRNA적표체차이무통계학의의(F치분별위1.86、1.69,P＞ 0.05),재저분화조、Ⅲ기급유림파결전이환자적폐조직중적표체현저저우상응적고분화조、Ⅰ～Ⅱ기급무림파결전이적환자(단백비교t치분별위4.31、3.92、3.41；mRNA비교t치분별위5.44、3.28、5.07,균P＜0.05).결론 FoxA 2가능대인류NSCLC적형성기억제작용,병삼여료종류적분화、림파결전이과정,차여폐암적림상분기상관.
Objective To investigate the expression and clinical significance of FoxA 2(hepatocyte nuclear factor 3β)in human non-small cell lung cancer(NSCLC)tissues and adjacent normal lung tissue.Methods The expressions of FoxA 2 protein in 80 cases with NSCLC tissues and adjacent normal lung tissue were tested by immunohistochemistry,the expressions of mRNA was detected by RT-PCR.Results The expressions of FoxA 2 both on protein and mRNA in NSCLC lung tissue were significantly less than those of adjacent normal lung tissue(protein expression comparison t value was 187.51,186.43,178.24 respectively;mRNA expression comparison t value was 236.70,260.13,126.27,respectively,P ＜ 0.05).However there was no difference on histopathological variability.F value equal to 1.86 and 1.69,P ＞ 0.05).In low differentiated group,the group of Ⅲ stage,with lymph node metastasis,the expression of FoxA 2 protein and mRNA was less than that in the group of high differentiated,the group of Ⅰ-Ⅱ stage,the group of non lymph node metastasis(pretein expression t values were 4.31,3.92,341,mRNA expression t values were 5.44,3.28,5.07,P＜0.05).Conclusion FoxA 2 might inhibit progression of human NSCLC.It may participate in the process of tumor tissue differentiation and lymph node metastasis in NSCLC.