中华医学美学美容杂志
中華醫學美學美容雜誌
중화의학미학미용잡지
CHINESE JOURNAL OF MEDICAL AESTHETICS AND COSMETOLOGY
2010年
6期
394-397
,共4页
氨基酮戊酸%光动力学疗法%角质形成细胞%细胞因子
氨基酮戊痠%光動力學療法%角質形成細胞%細胞因子
안기동무산%광동역학요법%각질형성세포%세포인자
Aminolevulinic acid%Photodynamic therapy (PDP)%Keratinocyte%Cytokine
目的 探讨不同强度光动力学反应对角质形成细胞(HaCat)分泌某些细胞因子的影响.方法 将0.0～12.8 mol/L的5-氨基酮戊酸(ALA)与HaCat细胞共同培养,检测胞内原卟啉Ⅸ(PpⅨ)含量;经632.8 nm激光照射后测定细胞死亡率.依据细胞死亡率(0%～10%)确定ALA浓度区间(0.0～1.6 mmol/L),继而检测该区间内细胞(包括照射组与非照射组)上清液中白介素-1α(IL-1α)、IL-6、碱性成纤维细胞生长因子(bFGF)、内皮素-1(ET-1)和α肿瘤坏死因子(TNF-α)的水平.结果 ALA浓度可调控胞内PpⅨ含量和光动力反应强度,在0.0～1.6 mmol/L:区间与细胞死亡率(≤10%)呈线性正相关(rs=0.451,r2=0.606,P＜0.05),且IL-1α、bFGF和TNF-α水平随着ALA浓度增加而逐渐升高(皆P＜0.05),IL-6和ET-1水平则无显著变化(皆P＞0.05).结论 通过增强光动力反应可促进HaCat细胞分泌某些细胞因子,以提高对某些皮肤病的疗效.
目的 探討不同彊度光動力學反應對角質形成細胞(HaCat)分泌某些細胞因子的影響.方法 將0.0～12.8 mol/L的5-氨基酮戊痠(ALA)與HaCat細胞共同培養,檢測胞內原卟啉Ⅸ(PpⅨ)含量;經632.8 nm激光照射後測定細胞死亡率.依據細胞死亡率(0%～10%)確定ALA濃度區間(0.0～1.6 mmol/L),繼而檢測該區間內細胞(包括照射組與非照射組)上清液中白介素-1α(IL-1α)、IL-6、堿性成纖維細胞生長因子(bFGF)、內皮素-1(ET-1)和α腫瘤壞死因子(TNF-α)的水平.結果 ALA濃度可調控胞內PpⅨ含量和光動力反應彊度,在0.0～1.6 mmol/L:區間與細胞死亡率(≤10%)呈線性正相關(rs=0.451,r2=0.606,P＜0.05),且IL-1α、bFGF和TNF-α水平隨著ALA濃度增加而逐漸升高(皆P＜0.05),IL-6和ET-1水平則無顯著變化(皆P＞0.05).結論 通過增彊光動力反應可促進HaCat細胞分泌某些細胞因子,以提高對某些皮膚病的療效.
목적 탐토불동강도광동역학반응대각질형성세포(HaCat)분비모사세포인자적영향.방법 장0.0～12.8 mol/L적5-안기동무산(ALA)여HaCat세포공동배양,검측포내원계람Ⅸ(PpⅨ)함량;경632.8 nm격광조사후측정세포사망솔.의거세포사망솔(0%～10%)학정ALA농도구간(0.0～1.6 mmol/L),계이검측해구간내세포(포괄조사조여비조사조)상청액중백개소-1α(IL-1α)、IL-6、감성성섬유세포생장인자(bFGF)、내피소-1(ET-1)화α종류배사인자(TNF-α)적수평.결과 ALA농도가조공포내PpⅨ함량화광동력반응강도,재0.0～1.6 mmol/L:구간여세포사망솔(≤10%)정선성정상관(rs=0.451,r2=0.606,P＜0.05),차IL-1α、bFGF화TNF-α수평수착ALA농도증가이축점승고(개P＜0.05),IL-6화ET-1수평칙무현저변화(개P＞0.05).결론 통과증강광동력반응가촉진HaCat세포분비모사세포인자,이제고대모사피부병적료효.
Objective To investigate the effects of photodynamic reaction on cytokines production of HaCat keratinocytes treated with 5-aminolevulinic acid (ALA). Methods The HaCat cells were cocultured in vitro with different concentrations of ALA. Three hours later PpIX levels in HaCat cells were detected, and phtotodynamic reaction was induced by 632.8 nm laser radiation on cells. The cell death was analyzed using a flow cytometer to evaluate reactive intensity. The cytokines contents in supernatant were determined, including interleukin (IL)- lα, IL-6, basic fibroblast growth factor (bFGF), endothelin (ET)-1 and tumor necrosis factor (TNF)-α. Results Different cellular PpIX levels and cell death rates were seen according to ALA concentrations. The cell death rate did not exceed 10% and showed a positive linear relationship with ALA concentration (P＜0.05), when the later was at 0.0-1.6 mmol/L interval. The levels of IL-1α, bFGF and TNF-α increased as ALA concentration ascended at this interval (P＜0.05). No significant correlation was found between ALA concentration and IL-6 or ET-1 level (P＞0.05). Conclusion The photodynamic reactive intensity could be enhanced by increasing ALA concentration. And at certain scope, higher reactive grade might induce higher levels of keratinocyte-derived cytokines.
