CAJ | 학술논문

为了探讨烟粉虱Remisia tabaci不同种群个体乙酰胆碱酯酶敏感性差异及其与抗药性的天系,我们选用室内饲养的烟粉虱SUD-S敏感品系和6个田间抗性种群,采用酶标板酶动力学法测定了各品系(种群)乙酰胆碱酯酶对抑制剂的敏感性反应以及抑制剂存在时各抗性种群个体乙酰胆碱酯酶残余活性频率分布.结果表明:在抑制剂浓度为300 μmol/L时,敏感品系乙酰胆碱酯酶的活性基本上被完伞抑制,可以明显地区分敏感品系与田问抗性种群.在抑制剂浓度为2 000 μmol/L时,各抗性种群个体乙酰胆碱酯酶残余活性频率分布差异明显,其中ZZ-R种群和FZ-R种群的乙酰胆碱酯酶残余活性频率分布相似,大部分个体的乙酰胆碱酯酶残余活性分布在1.00～1.80 mOD/min之间;SM-R种群和ND-R种群的乙酰胆碱酯酶残余活性频率分布也相似,大部分个体的乙酰胆碱酯酶残余活性分布在0.40～1.00 mOD/min之问;LY-R和NP-R种群大部分个体的乙酰胆碱酯酶残余活性分别分布在1.00～1.60 mOD/min和0.80～1.20 mOD/min之间.各抗性种群乙酰胆碱酯酶高残余活性(大于1.00 mOD/min)个体频率与对敌敌畏的抗性水平之间具有明显相父性,相关系数为0.86(P<0.05).考虑到乙酰胆碱酯酶对抑制剂作用不敏感是一些昆虫对有机磷和氨基甲酸酯类杀虫剂抗性的重要机制之一,建议可以将乙酰胆碱酯酶对敌敌畏的敏感性作为烟粉虱抗药性生化检测的一个参考指标.
위료탐토연분슬Remisia tabaci불동충군개체을선담감지매민감성차이급기여항약성적천계,아문선용실내사양적연분슬SUD-S민감품계화6개전간항성충군,채용매표판매동역학법측정료각품계(충군)을선담감지매대억제제적민감성반응이급억제제존재시각항성충군개체을선담감지매잔여활성빈솔분포.결과표명:재억제제농도위300 μmol/L시,민감품계을선담감지매적활성기본상피완산억제,가이명현지구분민감품계여전문항성충군.재억제제농도위2 000 μmol/L시,각항성충군개체을선담감지매잔여활성빈솔분포차이명현,기중ZZ-R충군화FZ-R충군적을선담감지매잔여활성빈솔분포상사,대부분개체적을선담감지매잔여활성분포재1.00～1.80 mOD/min지간;SM-R충군화ND-R충군적을선담감지매잔여활성빈솔분포야상사,대부분개체적을선담감지매잔여활성분포재0.40～1.00 mOD/min지문;LY-R화NP-R충군대부분개체적을선담감지매잔여활성분별분포재1.00～1.60 mOD/min화0.80～1.20 mOD/min지간.각항성충군을선담감지매고잔여활성(대우1.00 mOD/min)개체빈솔여대활활외적항성수평지간구유명현상부성,상관계수위0.86(P<0.05).고필도을선담감지매대억제제작용불민감시일사곤충대유궤린화안기갑산지류살충제항성적중요궤제지일,건의가이장을선담감지매대활활외적민감성작위연분슬항약성생화검측적일개삼고지표.
To study the difference in sensitivity of acetylcholinesterase (AChE) from individual adult female and its relationship with insecticide resistance in different populations of Bemisia tabaci, the response of AChE to inhibition by dichlorvos and the frequency distribution of residual activity in presence of inhibitor in the susceptible SUD-S strain and six field resistant populations of B. tabaci were measured by microtiter plate kinetic assay. The results showed that dichlorvos at 300 μmol/L completely inhibited AChE activity of the SUD-S strain, and clearly distinguished the SUD-S from the six resistant populations. Significant differences were observed in frequency distribution of the residual AChE activity in adult female individuals in the six field populations of B. tabaci in presence of 2 000 μmol/L dichlorvos. The frequency distribution patterns of the residual AChE activity in ZZ-R and ZZ-R populations were similar, and most of the residual activity in adult female individuals ranged from 1.00 to 1.80 mOD/min. SM-R and ND-R populations also had similar frequency distribution patterns of the residual AChE activity, and most of their residual activity in adult female individuals ranged from 0.40 to 1.00 mOD/min; whereas in LY-R and NP-R populations, most of the residual AChE activity in adult female individuals ranged from 1.00 to 1.80 mOD/min and 0.80 to 1.20 mOD/min, respectively. The correlation coefficient between the high residual AChE activity (higher than 1. 00 mOD/min) and the resistant level to dichlorvos was 0. 86 (P<0. 05). Since the insensitivity of AChE to inhibitor is one of the mechanisms of resistance to organophosphorus and carbamate insecticides in some insects, we suggest that the sensitivity of AChE to dichlorvos be used as a reference index for the biochemical detection of insecticide resistance in B. tabaci.