CAJ | 학술논문

以辛酸亚锡为催化剂, 1,4-丁二醇为引发剂, 制备出ε-己内酯/L-丙交酯共聚酯(PLCL). 以1-(3-二甲氨基丙基)-3-乙基碳二亚胺/N-羟基琥珀酰亚胺为缩合剂将肝素连接在PLCL两侧端基上. 采用 ~1H NMR技术测定了共聚酯端基的肝素化率;用XPS分析了肝素化后聚酯中N和S含量, 利用甲苯胺蓝紫外分光光度计法测定了表面肝素含量, 并根据静态接触角测定结果讨论了材料表面的亲水性变化. 凝血酶原时间、凝血酶时间和部分凝血活酶时间测试数据表明, 肝素化后PLCL的抗凝血性能得到明显改善. 探索了该共聚酯进行电纺丝加工的可行性.
이신산아석위최화제, 1,4-정이순위인발제, 제비출ε-기내지/L-병교지공취지(PLCL). 이1-(3-이갑안기병기)-3-을기탄이아알/N-간기호박선아알위축합제장간소련접재PLCL량측단기상. 채용 ~1H NMR기술측정료공취지단기적간소화솔;용XPS분석료간소화후취지중N화S함량, 이용갑분알람자외분광광도계법측정료표면간소함량, 병근거정태접촉각측정결과토론료재료표면적친수성변화. 응혈매원시간、 응혈매시간화부분응혈활매시간측시수거표명, 간소화후PLCL적항응혈성능득도명현개선. 탐색료해공취지진행전방사가공적가행성.
Heparin-conjugated biodegradable copolymers were synthesized by attaching heparin to the ends of the copolyesters of ε-caprolactone and L-lactide(PLCL) in the presence of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide. They were characterized by means of ~1H NMR, FTIR, XPS and UV-spectrophotometer techniques. The results indicate that heparin is successfully covalently coupled to PLCL, and the heparin-conjugated content is increased with increasing the ε-caprolactone molar content in the copolyesters. The PLCL surface after heparining became more hydrophilic than before due to the decrease of hydrophilic static contact angle. The clotting time of PLCL containing heparin was evaluated by activated partial thromboplastin time(APTT), thrombin time(TT) and prothrombin time(PT) measurements. The antithrombincity of the copolymers modified with heparin was improved among which the sample LC-90-H was the best. Finally, ultrafine fibers of LC-90-H were prepared by electrospinning process. It shows the promise to be applied in the inner-layer of tissue engineering blood vessel scaffolds.