CAJ | 학술논문

本文探索水稻幼根生长过程中ABA和GA对ROS代谢的影响,结果显示:低于0.5 μmol/L的ABA促进根伸长,超过此浓度的ABA抑制根伸长;H_2O_2与ABA相似,低浓度促进而高浓度抑制;在观察浓度范围内,GA促进根伸长.利用3,3'-二氨基联苯胺(diaminobenzidine, DAB)组织化学染色方法检测根尖部位H_2O_2代谢情况,结果显示,对照、ABA,GA和ABA+GA处理后,根尖分别呈现浅黄色、深棕色、黄色和深棕色,说明ABA比GA诱导较多H_2O_2产生.进一步研究发现,ABA和ABA+GA分别上调过氧化物酶(peroxidase, POD)活性13.4%和8.37%,GA下调POD活性19.0%;同时,ABA和ABA+GA处理分别下调过氧化氢酶(catalase, CAT)活性14.8%和16.3%,GA则上调CAT活性5.16%.以上结果表明,ABA可能通过上调POD和抑制CAT活性来提高ROS含量,而GA与之相反,严谨控制ROS浓度,促进水稻生长.
본문탐색수도유근생장과정중ABA화GA대ROS대사적영향,결과현시:저우0.5 μmol/L적ABA촉진근신장,초과차농도적ABA억제근신장;H_2O_2여ABA상사,저농도촉진이고농도억제;재관찰농도범위내,GA촉진근신장.이용3,3'-이안기련분알(diaminobenzidine, DAB)조직화학염색방법검측근첨부위H_2O_2대사정황,결과현시,대조、ABA,GA화ABA+GA처리후,근첨분별정현천황색、심종색、황색화심종색,설명ABA비GA유도교다H_2O_2산생.진일보연구발현,ABA화ABA+GA분별상조과양화물매(peroxidase, POD)활성13.4%화8.37%,GA하조POD활성19.0%;동시,ABA화ABA+GA처리분별하조과양화경매(catalase, CAT)활성14.8%화16.3%,GA칙상조CAT활성5.16%.이상결과표명,ABA가능통과상조POD화억제CAT활성래제고ROS함량,이GA여지상반,엄근공제ROS농도,촉진수도생장.
The results show that less than 0.5 μmol/L ABA accelerates the rice seeding root elongation, but more than 0.5 μmol/L ABA turns to inhibit the process. Similar to ABA, H_2O_2 accelerating turns to inhibiting the rice seeding root elongation at 1mmol/L concentration. GA accelerating rice root elongation in the all concentration is used. By diaminobenzidine (DAB) stains to detect the H_2O_2 accumulation in rice seeding roots, the results show that H_2O_2 accumulation are intensified by ABA or ABA +GA treatment but not by GA. Moreover, ABA and ABA +GA up-regulated the activity of peroxidase (POD) in 13.4% and 8.37%, respectively, but GA down-regulated the activity of POD in 19.0%. Moreover, the activity of catalase (CAT) is strongly down-regulated by ABA and ABA + GA in 14.8% and 16.3%, respectively, but GA up-regulated the activity of CAT in 5.16%. In a word, ABA and GA control the activities of both POD and CAT and thus regulating the H_2O_2 accumulation in rice seeding roots, suggesting that ABA and GA may modulate plant growth and development by mediating the ROS metabolism.