中国小儿急救医学
中國小兒急救醫學
중국소인급구의학
CHINESE PEDIATRIC EMERGENCY MEDICINE
2012年
4期
412-414
,共3页
姜黄素%急性肺损伤%高氧%大鼠,新生
薑黃素%急性肺損傷%高氧%大鼠,新生
강황소%급성폐손상%고양%대서,신생
Curcumin%Acute lung injury%Hyperoxia%Rat,newborn
目的 探讨姜黄素对新生大鼠高氧急性肺损伤的作用.方法 95％氧气3d制备新生大鼠高氧急性肺损伤模型,建模后将40只新生大鼠随机分为实验组[姜黄素灌胃,剂量为100 mg/(kg·d)]和对照组,每组20只.以20只正常新生大鼠作为空白组.在灌胃4d、7d后各组分别处死10只大鼠,取肺脏切片,检测肿瘤坏死因子(tumor necrosis factor,TNF)-α、白细胞介素(interleukin,IL)-Iβ及髓过氧化物酶( myeloperoxidase,MPO)含量.结果 实验组大鼠病理切片损伤均较对照组减轻.实验组大鼠4d及7d后TNF-α、IL-1β及MPO水平均明显低于对照组(P＜0.05);对照组大鼠4d、7d后TNF-α、IL-1β及MPO水平均高于空白组,差异有统计学意义(P＜0.01).结论 姜黄素对新生鼠高氧急性肺损伤可起保护作用.
目的 探討薑黃素對新生大鼠高氧急性肺損傷的作用.方法 95％氧氣3d製備新生大鼠高氧急性肺損傷模型,建模後將40隻新生大鼠隨機分為實驗組[薑黃素灌胃,劑量為100 mg/(kg·d)]和對照組,每組20隻.以20隻正常新生大鼠作為空白組.在灌胃4d、7d後各組分彆處死10隻大鼠,取肺髒切片,檢測腫瘤壞死因子(tumor necrosis factor,TNF)-α、白細胞介素(interleukin,IL)-Iβ及髓過氧化物酶( myeloperoxidase,MPO)含量.結果 實驗組大鼠病理切片損傷均較對照組減輕.實驗組大鼠4d及7d後TNF-α、IL-1β及MPO水平均明顯低于對照組(P＜0.05);對照組大鼠4d、7d後TNF-α、IL-1β及MPO水平均高于空白組,差異有統計學意義(P＜0.01).結論 薑黃素對新生鼠高氧急性肺損傷可起保護作用.
목적 탐토강황소대신생대서고양급성폐손상적작용.방법 95％양기3d제비신생대서고양급성폐손상모형,건모후장40지신생대서수궤분위실험조[강황소관위,제량위100 mg/(kg·d)]화대조조,매조20지.이20지정상신생대서작위공백조.재관위4d、7d후각조분별처사10지대서,취폐장절편,검측종류배사인자(tumor necrosis factor,TNF)-α、백세포개소(interleukin,IL)-Iβ급수과양화물매( myeloperoxidase,MPO)함량.결과 실험조대서병리절편손상균교대조조감경.실험조대서4d급7d후TNF-α、IL-1β급MPO수평균명현저우대조조(P＜0.05);대조조대서4d、7d후TNF-α、IL-1β급MPO수평균고우공백조,차이유통계학의의(P＜0.01).결론 강황소대신생서고양급성폐손상가기보호작용.
Objective To investigate the effect of curcumin on the hyperoxia-induced acute lung injury in neonatal rat.Methods A model of hyperoxia acute lung injury in neonatal rat was established by exposure to 95％ O2 for 3 days.After the model was established,40 rats were randomly divided into the treated group ( n =20) and the control group ( n =20).The 20 injured rats which were treated by curcumin ( 100 mg/( kg· d)were treated group,and the other 20 injured rats were control group.The 20 normal rats were blank group ( n =20).Lung tissues were collected for doing pathological section and measuring the levels of tumor necrosis factor(TNF)-a,interleukin(IL)-1β and myeloperoxidase(MPO) after 4 d and 7 d.Results The gross and micrographic injury of lung was milder in treated group than that in control group.The levels of TNF-α,IL-1β and MPO in treated group were significantly lower than those in control group at 4 d and 7 d ( P ＜0.05 ).Compared with the blank group,the levels of TNF-α,IL-1β and MPO in control group increased significantly at 4 d and 7 d ( P ＜ 0.01 ).Conclusion Curcumin can offer effective protection against acute lung injury induced by hyperoxia in neonatal rat.
