CAJ | 학술논문

目的探讨海马神经元损伤过程中第10染色体同源丢失性磷酸酶张力蛋白基因(phosphatase and tensin homology deleted on chromosome ten,PTEN)表达水平的变化与谷氨酸(Glu)的α-氨基羟甲基恶唑丙酸(AMPA)受体亚基表达定位变化的关系.方法选用新生SD大鼠原代培养海马神经元,建立神经元牵张损伤模型,采用Western blot和TUNEL染色检测神经元损伤各时相点PTEN表达的变化以及AMPA受体各亚基在细胞中的表达定位变化.结果神经元牵张损伤后,PTEN的表达增加,细胞总蛋白中AMPA受体GluR1、GluR2、GluR3亚基的表达水平损伤前后差异无统计学意义,但细胞膜中的AMPA受体GluR2亚基在牵张损伤后明显降低.结论海马神经元受到机械性牵张损伤后,细胞内PTEN水平与膜表面GluR2亚基表达变化相反.提示PTEN磷酸酶可能参与了AMPA受体中GluR2亚基的转运和再聚合过程.
목적 탐토해마신경원손상과정중제10염색체동원주실성린산매장력단백기인(phosphatase and tensin homology deleted on chromosome ten,PTEN)표체수평적변화여곡안산(Glu)적α-안기간갑기악서병산(AMPA)수체아기표체정위변화적관계.방법 선용신생SD대서원대배양해마신경원,건립신경원견장손상모형,채용Western blot화TUNEL염색검측신경원손상각시상점PTEN표체적변화이급AMPA수체각아기재세포중적표체정위변화.결과 신경원견장손상후,PTEN적표체증가,세포총단백중AMPA수체GluR1、GluR2、GluR3아기적표체수평손상전후차이무통계학의의,단세포막중적AMPA수체GluR2아기재견장손상후명현강저.결론 해마신경원수도궤계성견장손상후,세포내PTEN수평여막표면GluR2아기표체변화상반.제시PTEN린산매가능삼여료AMPA수체중GluR2아기적전운화재취합과정.
Objective To explore the relationship of phosphatase and tensin homology deleted on chromosome ten ( PTEN ) expression with the positioning of AMPA recepter subunit GluR2 in hippocampal neuron after stretch injury. Methods The primary rat hippocampal neuron from neonate rats were cultured and the model of stretch injury established. Western blot and TUNEL staining were used to detect PTEN expression and the positioning of AMPA receptor subunit GluR2 in rat hippocampal neuron at different time intervals after stretch injury. Results The PTEN expression was increased obviously after stretch injury, while the expressions of AMPA receptors GluR1, GluR2 and GluR3 showed no evident differences at every time point. However, AMPA receptor subunit GluR2 located at cytomembrane was down-regulated obviously after stretch injury. Conclusion The intracellular PTEN expression and the expression of AMPA receptor subunit GluR2 located at cytomembrane are on the contrary, indicating that PTEN phosphatase may participate in the transport and reintegration process of AMPA receptor subunit GluR2 located at cytomembrane.