CAJ | 학술논문

目的探讨亚低温对侧方液压冲击脑损伤大鼠海马钙蛋白酶Ⅱ及微管结合蛋白2(MAP2)mRNA的影响.方法雄性SD大鼠18只,麻醉固定后头皮正中切口,钻孔王入打击管,6只打击后电热毯维持正常体温3 h(常温脑损伤组),6只打击后采用冰屑降温法使肛温降至33℃并维持3 h(亚低温组).对照组6只不打击.3 h后通过Real-time PCB法检测海马钙蛋白酶Ⅱ、MAP2 mRNA变化.结果常温创伤组与对照组比较,钙蛋白酶ⅡmRNA相对表达量明显升高(P<0.01),亚低温组与常温创伤组比较,钙蛋白酶ⅡmRNA相对表达量明显降低,差异有统计学意义(P<0.01);常温创伤组与对照组比较,MAF2 mRNA相对表达量明显降低(P<0.01);亚低温组与常温创伤组比较,MAP2 mRNA相对表达量明显增高,差异有统计学意义(P<0.01).结论亚低温可能通过抑制钙蛋白酶Ⅱ的活性减轻细胞骨架降解起到神经保护作用.
목적 탐토아저온대측방액압충격뇌손상대서해마개단백매Ⅱ급미관결합단백2(MAP2)mRNA적영향.방법웅성SD대서18지,마취고정후두피정중절구,찬공왕입타격관,6지타격후전열담유지정상체온3 h(상온뇌손상조),6지타격후채용빙설강온법사항온강지33℃병유지3 h(아저온조).대조조6지불타격.3 h후통과Real-time PCB법검측해마개단백매Ⅱ、MAP2 mRNA변화.결과상온창상조여대조조비교,개단백매ⅡmRNA상대표체량명현승고(P<0.01),아저온조여상온창상조비교,개단백매ⅡmRNA상대표체량명현강저,차이유통계학의의(P<0.01);상온창상조여대조조비교,MAF2 mRNA상대표체량명현강저(P<0.01);아저온조여상온창상조비교,MAP2 mRNA상대표체량명현증고,차이유통계학의의(P<0.01).결론아저온가능통과억제개단백매Ⅱ적활성감경세포골가강해기도신경보호작용.
Objective To study the early expression of ca]pain Ⅱ and microtubule associated protein 2 (MAP2) mRNA in the hippocampus of the lateral fluid percussion injury rats. Methods 18 Male Sprague-Dawley rats were randomly divided into 3 groups. The changes of Calpaln Ⅱ and MAP2 mRNA in hippocampus 3 h after injury were detected by real-time PCR. Results Compared to the control group (n = 6), the expression for Ca]pain Ⅱ mRNA increased obviously(P <0.01)in the lateral fluid percussion injury group(n=6) ,the expression for MAP2 mRNA degraded obviously(P <0.01). Compared with the lateral fluid percussion injury group(n =6) ,the expression for calpuin Ⅱ mRNA in the mild hypothermia group degraded obviously (n = 6), the expression for MAP2 mRNA increased obviously(P <0.01). Conclusion Mild hypothermia may act as neuroprotection by inhibiting the expression of Ca]pain Ⅱ and easing the degradation of cytoskeleton.