中华临床感染病杂志
中華臨床感染病雜誌
중화림상감염병잡지
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
2009年
5期
293-296
,共4页
王志刚%邵平扬%吴晓燕%倪侃翔%陆红霞
王誌剛%邵平颺%吳曉燕%倪侃翔%陸紅霞
왕지강%소평양%오효연%예간상%륙홍하
弧菌%创伤%培养%鉴定%药物敏感性试验
弧菌%創傷%培養%鑒定%藥物敏感性試驗
호균%창상%배양%감정%약물민감성시험
Vibrio vulnificus%Cultivation%Identification%Antibacterial susceptibility tests
目的 了解创伤弧菌的培养特征、生化反应特性和药物敏感性,以便提高临床检出率,降低病死率.方法 收集分离自浙江省嘉兴市第二医院临床死亡病例的创伤弧菌3株,对其进行培养鉴定和药物敏感性试验.结果 创伤弧菌在血琼脂中培养呈灰暗、不透明、β-溶血菌落.生化反应乳糖、纤维二糖、O/129(10μg)阳性;肌醇、鼠李糖等阴性.药物敏感性试验显示其对环丙沙星、左氧氟沙星、亚胺培南、头饱吡肟、头孢他啶、头孢曲松、复方磺胺甲恶唑、呋喃妥冈敏感,对庆大霉素、妥布霉素、氨曲南和头孢唑啉耐药.结论 创伤弧菌可从患者的血液、疱液及粪便样本中分离到,快速检测、早期诊断,并及时给予经验性抗菌药物治疗对降低病死率至关重要.
目的 瞭解創傷弧菌的培養特徵、生化反應特性和藥物敏感性,以便提高臨床檢齣率,降低病死率.方法 收集分離自浙江省嘉興市第二醫院臨床死亡病例的創傷弧菌3株,對其進行培養鑒定和藥物敏感性試驗.結果 創傷弧菌在血瓊脂中培養呈灰暗、不透明、β-溶血菌落.生化反應乳糖、纖維二糖、O/129(10μg)暘性;肌醇、鼠李糖等陰性.藥物敏感性試驗顯示其對環丙沙星、左氧氟沙星、亞胺培南、頭飽吡肟、頭孢他啶、頭孢麯鬆、複方磺胺甲噁唑、呋喃妥岡敏感,對慶大黴素、妥佈黴素、氨麯南和頭孢唑啉耐藥.結論 創傷弧菌可從患者的血液、皰液及糞便樣本中分離到,快速檢測、早期診斷,併及時給予經驗性抗菌藥物治療對降低病死率至關重要.
목적 료해창상호균적배양특정、생화반응특성화약물민감성,이편제고림상검출솔,강저병사솔.방법 수집분리자절강성가흥시제이의원림상사망병례적창상호균3주,대기진행배양감정화약물민감성시험.결과 창상호균재혈경지중배양정회암、불투명、β-용혈균락.생화반응유당、섬유이당、O/129(10μg)양성;기순、서리당등음성.약물민감성시험현시기대배병사성、좌양불사성、아알배남、두포필우、두포타정、두포곡송、복방광알갑악서、부남타강민감,대경대매소、타포매소、안곡남화두포서람내약.결론 창상호균가종환자적혈액、포액급분편양본중분리도,쾌속검측、조기진단,병급시급여경험성항균약물치료대강저병사솔지관중요.
Objective To investigate the cultivation, biochemical features and drug susceptibilities of Vibrio vulnificus. Methods Three strains of Vibrio vulnificus were isolated from fatal patients in the Second Hospital of Jiaxing, Zhejiang province. Cultivation, identification and antibacterial susceptibility test were performed. Results Vibrio vulnificus grew on blood agar as dull-gray, opaque colonies with β-hemolysis. The organism presented positive in lactose, cellobiose fermentation and O/129 (10 μg) tests, but lack of inositol and rhamnus. The antibacterial susceptibility tests showed that Vibrio vulnificus strains were sensitive to ciprofloxacin, levofloxacin, imipenem, cefepime, ceftazidime, ceftriaxone, compound sulfamethoxazole and nitrofurantoin, however, resistant to gentamicin, tobramycin, aztreonam and cefazolin. Conclusions Vibrio vulnificus can be isolated from blood, bubbles fluid, and stool. Rapid identification, early diagnosis, and prompt empirical antibacterial therapy are important for reducing the mortality.