CAJ | 학술논문

目的建立0～1 Gy范围内60Coγ射线、252Cf中子、单能中子及12C重离子诱发染色体畸变剂量-效应曲线,用于评价职业受照或低剂量辐射损伤.方法采用不同照射装置1 Gy以内照射离体人外周血,于培养开始加秋水仙素,培养48 h收获,制备染色体标本,Metafer中期细胞自动寻找系统,人机交互计数染色体畸变,最优拟合数据得到相应模型.结果在低剂量范围内,以双着丝粒体+环(dic+r)为终点,60C0γ射线为线性模型,252Cf中子、单能中子及12C重离子为线性平方模型；以无着丝粒断片( ace)为终点,60C0 γ射线和12C重离子为线性平方模型,252Cf中子和单能中子为线性模型；以总畸变为终点,4种射线均为线性平方模型.结论高传能线密度(LET)射线损伤效应高于低LET射线,4种射线损伤效应依次为252Cf混合中子＞单能中子＞12C重离子＞60C0 γ射线.在低剂量范围内dic+r指标适用于高LET照射的剂量估算.
목적 건립0～1 Gy범위내60Coγ사선、252Cf중자、단능중자급12C중리자유발염색체기변제량-효응곡선,용우평개직업수조혹저제량복사손상.방법 채용불동조사장치1 Gy이내조사리체인외주혈,우배양개시가추수선소,배양48 h수획,제비염색체표본,Metafer중기세포자동심조계통,인궤교호계수염색체기변,최우의합수거득도상응모형.결과 재저제량범위내,이쌍착사립체+배(dic+r)위종점,60C0γ사선위선성모형,252Cf중자、단능중자급12C중리자위선성평방모형；이무착사립단편( ace)위종점,60C0 γ사선화12C중리자위선성평방모형,252Cf중자화단능중자위선성모형；이총기변위종점,4충사선균위선성평방모형.결론 고전능선밀도(LET)사선손상효응고우저LET사선,4충사선손상효응의차위252Cf혼합중자＞단능중자＞12C중리자＞60C0 γ사선.재저제량범위내dic+r지표괄용우고LET조사적제량고산.
Objective To establish the dose-effect curves of chromosome aberrations in human peripheral blood lymphocytes induced by different LET rays,including 60Co γ-rays,252Cf neutrons,14MeV neutrons and 12C heavy ions at low doses,respectively,in order to assess radiation-induced damages after occupational and accidental exposure.Methods Heparinized whole blood samples were irradiated with the various radiation devices and doses ranged from 0 to 1 Gy with the interval of 0.25 Gy.Conventional chromosome culture method was applied with adding colchicines at the beginning and chromosome specimens were prepared after 48 h incubation.The Metafer scanning system was used for automatical finder of chromosome metaphases.The mathematic models were fitted according to aberration data obtained.Results At the dose range of 0 - 1 Gy,the math models of dic + r were linear for 60 Co γrays and linear-quadratics for 252 Cf neutrons,14 MeV neutrons and 12 C heavy ions.The models of ace were linear-quadratic for 60Co γ-rays and 12C heavy ion beams,and linear for 252Cf and 14 MeV neutrons.The models of total aberrations were linear-quadratic for all types of radiation.Conclusions High LET rays have higher biological effects in inducing chromosome aberrations yields compared with low LET rays.Moreover,the severity of damage is 252Cf ＞ 14 MeV neutrons ＞ 12C heavy ions ＞ 60Co γ-rays in turn.Therefore,in the range of low doses,the dic + r may be a better target of radiation damage for high LET radiation.