中华结核和呼吸杂志
中華結覈和呼吸雜誌
중화결핵화호흡잡지
Chinese Journal of Tuberculosis and Respiratory Diseases
2011年
2期
117-119
,共3页
吴龙章%潘美玉%刘欣%周海明%吴幸怡%罗春明
吳龍章%潘美玉%劉訢%週海明%吳倖怡%囉春明
오룡장%반미옥%류흔%주해명%오행이%라춘명
分枝杆菌,结核%硝基苯甲酸盐%药物耐受性
分枝桿菌,結覈%硝基苯甲痠鹽%藥物耐受性
분지간균,결핵%초기분갑산염%약물내수성
Mycobacterium tuberculosis%Nitrobenzenes%Drug tolerance
目的 观察和分析MTB对对硝基苯甲酸(PNB)的耐受情况,为实验室鉴定分枝杆菌菌种提供依据.方法 菌株来源为广州市胸科医院门诊和住院患者,从1449株分枝杆菌中确定MTB菌株1114株,对PNB培养基上生长的分枝杆菌,采用MPB64单克隆抗体试剂或进行传统生化试验,以区分MTB和非结核分枝杆菌群(NTM).实验组菌株58株为在PNB培养基上生长的分枝杆菌,但经传统生化试验义疑似MTB,经传统生化试验证实为MTB菌株后,再进行PNB最小抑菌浓度(MIC)试验,以观察其对PNB的耐受性;对照组菌株10株为在PNB鉴别培养基上不生长,经传统生化试验证实为MTB菌株;另选择H37Rv标准株和鸟分枝杆菌标准株及5株临床NTM分离株进行PNB药物的MIC值检测.两组间比较采用成组t值检验.结果 MTB菌株对PNB的耐药率为5.21%(58/1114),实验组菌株PNB的MIC值为1.0~1.5 g/L,而对照组PNB的MIC值为0.25~0.5 g/L,H37Rv标准株PNB的MIC值为0.25g/L,鸟分枝杆菌标准株和5株NTM临床分离株PNB的MIC则>2.0g/L,实验组与其他各组比较,差异均有统计学意义(t值为4.87~6.68,均P<0.01).结论 在采用PNB培养基进行分枝杆菌菌群初筛时,如出现菌株对PNB耐药,但菌落形态呈米黄色、粗糙呈菜花样 等,以及对一线抗结核药物敏感等符合MTB的特征时,必须进行传统的生化试验,或使用MPB64单克隆抗体试验进行菌型鉴定,或利用MTB对温度较为敏感的特点及药敏结果进行判断,以免将MTB误判为NTM导致误诊.
目的 觀察和分析MTB對對硝基苯甲痠(PNB)的耐受情況,為實驗室鑒定分枝桿菌菌種提供依據.方法 菌株來源為廣州市胸科醫院門診和住院患者,從1449株分枝桿菌中確定MTB菌株1114株,對PNB培養基上生長的分枝桿菌,採用MPB64單剋隆抗體試劑或進行傳統生化試驗,以區分MTB和非結覈分枝桿菌群(NTM).實驗組菌株58株為在PNB培養基上生長的分枝桿菌,但經傳統生化試驗義疑似MTB,經傳統生化試驗證實為MTB菌株後,再進行PNB最小抑菌濃度(MIC)試驗,以觀察其對PNB的耐受性;對照組菌株10株為在PNB鑒彆培養基上不生長,經傳統生化試驗證實為MTB菌株;另選擇H37Rv標準株和鳥分枝桿菌標準株及5株臨床NTM分離株進行PNB藥物的MIC值檢測.兩組間比較採用成組t值檢驗.結果 MTB菌株對PNB的耐藥率為5.21%(58/1114),實驗組菌株PNB的MIC值為1.0~1.5 g/L,而對照組PNB的MIC值為0.25~0.5 g/L,H37Rv標準株PNB的MIC值為0.25g/L,鳥分枝桿菌標準株和5株NTM臨床分離株PNB的MIC則>2.0g/L,實驗組與其他各組比較,差異均有統計學意義(t值為4.87~6.68,均P<0.01).結論 在採用PNB培養基進行分枝桿菌菌群初篩時,如齣現菌株對PNB耐藥,但菌落形態呈米黃色、粗糙呈菜花樣 等,以及對一線抗結覈藥物敏感等符閤MTB的特徵時,必鬚進行傳統的生化試驗,或使用MPB64單剋隆抗體試驗進行菌型鑒定,或利用MTB對溫度較為敏感的特點及藥敏結果進行判斷,以免將MTB誤判為NTM導緻誤診.
목적 관찰화분석MTB대대초기분갑산(PNB)적내수정황,위실험실감정분지간균균충제공의거.방법 균주래원위엄주시흉과의원문진화주원환자,종1449주분지간균중학정MTB균주1114주,대PNB배양기상생장적분지간균,채용MPB64단극륭항체시제혹진행전통생화시험,이구분MTB화비결핵분지간균군(NTM).실험조균주58주위재PNB배양기상생장적분지간균,단경전통생화시험의의사MTB,경전통생화시험증실위MTB균주후,재진행PNB최소억균농도(MIC)시험,이관찰기대PNB적내수성;대조조균주10주위재PNB감별배양기상불생장,경전통생화시험증실위MTB균주;령선택H37Rv표준주화조분지간균표준주급5주림상NTM분리주진행PNB약물적MIC치검측.량조간비교채용성조t치검험.결과 MTB균주대PNB적내약솔위5.21%(58/1114),실험조균주PNB적MIC치위1.0~1.5 g/L,이대조조PNB적MIC치위0.25~0.5 g/L,H37Rv표준주PNB적MIC치위0.25g/L,조분지간균표준주화5주NTM림상분리주PNB적MIC칙>2.0g/L,실험조여기타각조비교,차이균유통계학의의(t치위4.87~6.68,균P<0.01).결론 재채용PNB배양기진행분지간균균군초사시,여출현균주대PNB내약,단균락형태정미황색、조조정채화양 등,이급대일선항결핵약물민감등부합MTB적특정시,필수진행전통적생화시험,혹사용MPB64단극륭항체시험진행균형감정,혹이용MTB대온도교위민감적특점급약민결과진행판단,이면장MTB오판위NTM도치오진.
Objective To investigate the resistance of Mycobacterium tuberculosis ( MTB ) to p-nitrobenzoic acid ( PNB ), in order to provide the scientific basis for reliable identification of laboratory strains of mycobacterium. Methods Strains of mycobacterium grown in PNB media were identified with additional traditional biochemical tests, according to the standard protocols of laboratory diagnostics for tuberculosis by the Chinese Antituberculosis Association. For mycobacteria grown in the PNB media but highly suspected as MTB by traditional biochemical tests, MPB64 monoclonal antibody was used for the differentiation between MTB and non-tuberculosis mycobacteria group ( NTM ). Minimal inhibitory concentrations ( MIC ) of PNB was further determined for culture-confirmed MTB, 10 strains of clinically isolated MTB ( control ), H37 Rv standard strain, mycobacterium avium standard strain, and 5 strains of clinically isolated NTM. Results A total of 1114 strains of MTB were confirmed, among which 58 PNB manifested resistance. The rate of resistance was 5.21% ( 58/1114 ), with an MIC ranging for 1. 0 - 1.5g/L. The MICs of control MTB and H37 Rv standard strain were 0. 25 - 0. 5 g/L and 0. 25 g/L, respectively.Both mycobacterium avium standard strain and clinically isolated NTM showed an MIC of > 2. 0 g/L.Differences between groups were statistically significant ( t = 4. 87, 5.09, 6. 68, respectively, P < 0. 01 ).Conclusion In order to avoid laboratory misdiagnosis, for primary screening with NTB with PNB culture,the presence of MTB characteristics, including cream-colored broccoli-like colony morphologies, as well as clinical response to first-line anti-tuberculosis medications, despite PNB tolerance, warrants further investigations of traditional biochemical tests, differentiation with MPB64 monoclonal antibodies, or simply by the use of temperature manipulation or drug-sensitivity test results.