肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2008年
6期
390-394
,共5页
张雁钢%吕红凯%王东文%米振国
張雁鋼%呂紅凱%王東文%米振國
장안강%려홍개%왕동문%미진국
肾肿瘤%NF-κB%环氧化合物%免疫组织化学
腎腫瘤%NF-κB%環氧化閤物%免疫組織化學
신종류%NF-κB%배양화합물%면역조직화학
Kidney neoplasms%NF-kappa B%Epoxy compounds%Immunohistochemistry
目的 研究NF-κB、环氧合酶-2(COX-2)在肾癌中的表达,探讨其与肾癌的发生、发展、转移以及患者预后之间的关系.方法 应用免疫组织化学EnVison二步法对48例肾癌组织及5例正常肾组织中NF-κ B、COX-2的表达情况进行检测,并通过对患者随访,探讨二者与肾癌临床病理学参数及患者预后之间的关系.结果 NF-κ B、COX-2在肾癌组织中的表达均较正常组织明显增高(P<0.05).NF-κ B的表达与肾癌的临床分期之间呈显著的正相关关系(P<0.05),而COX-2的表达与肾癌的临床分期之间无显著相关性(P>0.05).NF-κ B的表达与肾癌病理分级之间无显著相关性(P>0.05).而COX-2的表达与肾癌的病理分级之间呈显著的负相关关系(P<0.05).NF-κ B表达阳性者的生存率明显低于NF-κB表达阴性者(P<0.05),而COX-2表达阴性者的生存率低于COX-2表达阳性者,但差异无统计学意义(P>0.05).NF-κ B与COX-2在肾癌组织中表达差异无统计学意义(P>0.05).结论 NF-κ B、COX-2的表达可能与肾癌的发生、发展有关,NF-κ B的表达可能还与肾癌的转移及患者的预后有关,二者有望作为肾癌早期诊断和判断肾癌患者预后的辅助指标.
目的 研究NF-κB、環氧閤酶-2(COX-2)在腎癌中的錶達,探討其與腎癌的髮生、髮展、轉移以及患者預後之間的關繫.方法 應用免疫組織化學EnVison二步法對48例腎癌組織及5例正常腎組織中NF-κ B、COX-2的錶達情況進行檢測,併通過對患者隨訪,探討二者與腎癌臨床病理學參數及患者預後之間的關繫.結果 NF-κ B、COX-2在腎癌組織中的錶達均較正常組織明顯增高(P<0.05).NF-κ B的錶達與腎癌的臨床分期之間呈顯著的正相關關繫(P<0.05),而COX-2的錶達與腎癌的臨床分期之間無顯著相關性(P>0.05).NF-κ B的錶達與腎癌病理分級之間無顯著相關性(P>0.05).而COX-2的錶達與腎癌的病理分級之間呈顯著的負相關關繫(P<0.05).NF-κ B錶達暘性者的生存率明顯低于NF-κB錶達陰性者(P<0.05),而COX-2錶達陰性者的生存率低于COX-2錶達暘性者,但差異無統計學意義(P>0.05).NF-κ B與COX-2在腎癌組織中錶達差異無統計學意義(P>0.05).結論 NF-κ B、COX-2的錶達可能與腎癌的髮生、髮展有關,NF-κ B的錶達可能還與腎癌的轉移及患者的預後有關,二者有望作為腎癌早期診斷和判斷腎癌患者預後的輔助指標.
목적 연구NF-κB、배양합매-2(COX-2)재신암중적표체,탐토기여신암적발생、발전、전이이급환자예후지간적관계.방법 응용면역조직화학EnVison이보법대48례신암조직급5례정상신조직중NF-κ B、COX-2적표체정황진행검측,병통과대환자수방,탐토이자여신암림상병이학삼수급환자예후지간적관계.결과 NF-κ B、COX-2재신암조직중적표체균교정상조직명현증고(P<0.05).NF-κ B적표체여신암적림상분기지간정현저적정상관관계(P<0.05),이COX-2적표체여신암적림상분기지간무현저상관성(P>0.05).NF-κ B적표체여신암병리분급지간무현저상관성(P>0.05).이COX-2적표체여신암적병리분급지간정현저적부상관관계(P<0.05).NF-κ B표체양성자적생존솔명현저우NF-κB표체음성자(P<0.05),이COX-2표체음성자적생존솔저우COX-2표체양성자,단차이무통계학의의(P>0.05).NF-κ B여COX-2재신암조직중표체차이무통계학의의(P>0.05).결론 NF-κ B、COX-2적표체가능여신암적발생、발전유관,NF-κ B적표체가능환여신암적전이급환자적예후유관,이자유망작위신암조기진단화판단신암환자예후적보조지표.
Objective To study the expression of NF-κB and COX-2 protein in renal cell carcinoma, and to investigate the clinical value of it in the occurrence, progression and metastasis of renal cell carcinoma as well as the prognosis of patients. Methods 48 cases of renal cell carcinoma tissues and 5 cases of normal renal tissues were detected by using immunohistochemical method, and following up the patients who were included in the study. Then we analyzed the relationship between NF-κB and COX-2 protein level and clinic pathological parameters as well as the prognosis of patients. Resalts The expression of NF-κB and COX-2 protein were increasing in renal cell carcinoma, the protein levels were significantly different compared with that in normal renal tissues(P <0.05). There were significantly positive correlation between the expression of NF-κB protein and the clinic stages of renal cell earcinoma(P <0.05), but no correlation between the expression of COX-2 protein and them(P >0.05). There were significantly negative correlation between the expression of COX-2 protein and the pathological grades of renal cell careinoma(P <0.05), but no correlation between the expression of NF-κB protein and them (P>O.05). The survival rate of these patients whose expression of NF-κB protein were positive was significantly lower than those of negative expression of NF-κB protein(P <0.05), and there was opposite result for COX-2, but not significanfly(P >0.05). The expression of NF-κB and COX-2 protein in renal cell carcinoma had no significant correlation (P >0.05). Conclusion There may be relationship between the expression of NF-κB and COX-2 protein and the occurrence, progression of renal cell carcinoma, the expression of NF-κB may also have association with metastasis of renal cell carcinoma as well as the prognosis of patients. Detecting the expression of NF-κB and COX-2 protein may be useful in early diagnosis of renal cell carcinoma as well as prognosis evaluation of patients.
