CAJ | 학술논문

目的探讨去细胞结合光氧化技术处理后的牛颈静脉带瓣管道(BJVC)在体内的抗钙化性能.方法取新鲜牛颈静脉20根,每根裁出4个带瓣血管片,随机分为A、B、c、D 4组,分别以染料介导光氧化、戊二醛、去细胞及去细胞结合光氧化4种技术处理.建立大鼠皮下包埋模型,2个月后获取组织标本,原子吸收光谱法测定组织钙含量;并应用X线、CT、骨密度扫描检测整体组织钙化情况.结果 (1)D组试片标本形态较完整,柔韧性好,无结节样改变;其整体组织钙盐密度明显低于A组及B组,与C组接近.(2)D组管壁组织钙含量显著低于A组及B组(P＜0.01),与C组差异无统计学意义(P＞0.05);D组瓣膜组织的钙含量显著低于B组(P＜0.01),与A组及c组比较均差异无统计学意义(P＞0.05).结论去细胞结合光氧化技术与传统戊二醛及染料介导光氧化技术比较,能明显提高牛颈静脉体内抗钙化性能.
목적 탐토거세포결합광양화기술처리후적우경정맥대판관도(BJVC)재체내적항개화성능.방법 취신선우경정맥20근,매근재출4개대판혈관편,수궤분위A、B、c、D 4조,분별이염료개도광양화、무이철、거세포급거세포결합광양화4충기술처리.건립대서피하포매모형,2개월후획취조직표본,원자흡수광보법측정조직개함량;병응용X선、CT、골밀도소묘검측정체조직개화정황.결과 (1)D조시편표본형태교완정,유인성호,무결절양개변;기정체조직개염밀도명현저우A조급B조,여C조접근.(2)D조관벽조직개함량현저저우A조급B조(P＜0.01),여C조차이무통계학의의(P＞0.05);D조판막조직적개함량현저저우B조(P＜0.01),여A조급c조비교균차이무통계학의의(P＞0.05).결론 거세포결합광양화기술여전통무이철급염료개도광양화기술비교,능명현제고우경정맥체내항개화성능.
Objective To study the anti-calcification properties of BJVC treated by dye-mediated photooxidation following decellularization in vivo.Methods Each of twenty fresh bovine jugular veins with a retained native valve procured from a slaughterhouse was cut into 4 trial patches with valve,which were randomly divided into greup A,B,C and D,which were treated respectively by the technology of dye-mediated photooxidation,glutaraldehyde,decellularization and dye-mediated photooxidantion following decellularization.One of each group trial patches were implanted subcutaneously in the same Wistar rat.60 days after implantation,all trial rats were sacrificed and the specimens were retrieved.The tissue calcium content was determined by flame atomic absorption spectrophotometer.The tissue calcium salt distribution was evaluated by X-ray,spiral computed tomography,bone density scanning.Results (1)The trial patches in D group were relative integrity,soft and tenacious,no nodes emerged,its calcium salts density in total was significantly less than those of group A and B,and was similar to group C. (2)The calcium content of vessel wall in group D was significantly lower than those of group A and B (P＜0.01),and was no different with which of group (P＞0.05).The calcium content in the valves in group D was lower than that of group B(P＜0.01),and was no different with those of group A and D (P＞O.05).Conclusion The technology of dye-mediated photooxidation following decellarization can effectively prevent the calcification of bovine jugular venous conduit in vivo.