CAJ | 학술논문

具有双重营养缺陷型的裂褶菌单核体突变菌株的原生质体经过灭活处理后作为供体与同样带有营养缺陷型标记的风尾菇单核体原生质体融合, 得到大量生长速度和菌落形态差异较大的多核体和双核体融合子. 这些融合子主要显示了供体菌株的特性, 数次转接后, 有的融合子从多核体转变成双核体, 有的融合子完全恢复了供体亲本双核体的遗传特性, 同时获得了一些有意义值得继续探讨的新的生理特性, 如菌丝体在木屑麸皮以及废棉培养料上生长速度较快, 在平板培养基和木屑培养料上均较早较快地形成原基和子实体等. 结果表明原生质体非对称融合是一种效率较高的融合方式, 可以作为食用菌育种的一种有效途经.
구유쌍중영양결함형적렬습균단핵체돌변균주적원생질체경과멸활처리후작위공체여동양대유영양결함형표기적풍미고단핵체원생질체융합, 득도대량생장속도화균락형태차이교대적다핵체화쌍핵체융합자. 저사융합자주요현시료공체균주적특성, 수차전접후, 유적융합자종다핵체전변성쌍핵체, 유적융합자완전회복료공체친본쌍핵체적유전특성, 동시획득료일사유의의치득계속탐토적신적생리특성, 여균사체재목설부피이급폐면배양료상생장속도교쾌, 재평판배양기화목설배양료상균교조교쾌지형성원기화자실체등. 결과표명원생질체비대칭융합시일충효솔교고적융합방식, 가이작위식용균육충적일충유효도경.
A large number of fusion products from polarized protoplast fusion between inactivated monokaryotic protoplasts of Schizophyllum commune and viable monokaryotic protoplasts of Pleurotus sajor-caju were produced. The preliminary study showed that the fusion products mainly carried the characteristics of donor parent instead of recipient parent. After several subcultures, some fusants produced clamp connections and microscopical observation confirmed there were twin nuclei in their mycelial cells. The result implied that the dead donor could re-emerge from the fusant and companed with the transformation from the monokaryon to dikaryon, as well as achieve some new characteristics such as mycelial growth fast and easier and earlier fruiting. The result also implied that polarized protoplast fusion seems to be a more efficient method to produce fusion products in strain improvement of edible fungi.