CAJ | 학술논문

采用溶剂浮选法对大黄提取液中的芦荟大黄素、大黄素、大黄酚以及大黄素甲醚进行了分离富集,并用高效液相色谱法分别测定了其含量.考察了料液浓度、浮选溶剂、浮选时间、浮选液pH值、氮气流速和电解质NaCl浓度对浮选效率的影响,并与泡沫浮选法和溶剂萃取法进行了比较.结果表明:溶剂萃取效果最差,泡沫浮选次之,溶剂浮选法分离富集效果最好.当料液浓度为6.4 mg/mL, 浮选时间为30 min,浮选液pH=1～2,氮气流速为20 mL/min,电解质NaCl浓度为0.4 mol/L时,溶剂浮选效率最佳.
채용용제부선법대대황제취액중적호회대황소、대황소、대황분이급대황소갑미진행료분리부집,병용고효액상색보법분별측정료기함량.고찰료료액농도、부선용제、부선시간、부선액pH치、담기류속화전해질NaCl농도대부선효솔적영향,병여포말부선법화용제췌취법진행료비교.결과표명:용제췌취효과최차,포말부선차지,용제부선법분리부집효과최호.당료액농도위6.4 mg/mL, 부선시간위30 min,부선액pH=1～2,담기류속위20 mL/min,전해질NaCl농도위0.4 mol/L시,용제부선효솔최가.
The active constituents of Radix et Rhizoma Rhei were separated and enriched by solvent flotation and high performance liquid chromatography (HPLC) was applied to determine four major active constituents(aloe-emodin,emodin,chrysophanol,and physcion) .The effects of flotation solvent, pH of the solution, the flow rate of nitrogen gas, flotation time and the concentration of NaCl on the flotation efficiency were investigated. Solvent flotation was compared with foam flotation and solvent extraction. The experimental results showed that the highest efficiency of separation and enrichment were obtained by solvent flotation. The optimum conditions for the flotation solvent are the concentration feed of 6.4 mg/mL, flotation time of 30 min,pH of 1～2,the flow rate of nitrogen gas of 20 mL/min, the concentration of NaCl of 0.4 mol/L.