生物技术通报
生物技術通報
생물기술통보
BIOTECHNOLOGY BULLETIN
2010年
1期
99-102,117
,共5页
高效氯氰菊酯%铜绿假单胞菌%响应面法%降解条件优化
高效氯氰菊酯%銅綠假單胞菌%響應麵法%降解條件優化
고효록청국지%동록가단포균%향응면법%강해조건우화
Beta-cypermethrin%Pseudomonas aeruginosa%Response surface methodology%Degrading condition optimization
从农药厂活性污泥中,分离到一株能以高效氯氰菊酯为唯一碳源生长的细菌CH7.经生理生化试验和16S rDNA分析,将菌株CH7鉴定为铜绿假单胞菌(Pseudomonas aeruginosa).采用Box-behnken设计试验、响应面法(response surface methodology)优化菌株CH7的降解条件.在最优条件下(29.4°C,pH7.0,接种量0.15 g/L),菌株CH7在12 d内对100 mg/L高效氯氰菊酯的降解率为90%.
從農藥廠活性汙泥中,分離到一株能以高效氯氰菊酯為唯一碳源生長的細菌CH7.經生理生化試驗和16S rDNA分析,將菌株CH7鑒定為銅綠假單胞菌(Pseudomonas aeruginosa).採用Box-behnken設計試驗、響應麵法(response surface methodology)優化菌株CH7的降解條件.在最優條件下(29.4°C,pH7.0,接種量0.15 g/L),菌株CH7在12 d內對100 mg/L高效氯氰菊酯的降解率為90%.
종농약엄활성오니중,분리도일주능이고효록청국지위유일탄원생장적세균CH7.경생리생화시험화16S rDNA분석,장균주CH7감정위동록가단포균(Pseudomonas aeruginosa).채용Box-behnken설계시험、향응면법(response surface methodology)우화균주CH7적강해조건.재최우조건하(29.4°C,pH7.0,접충량0.15 g/L),균주CH7재12 d내대100 mg/L고효록청국지적강해솔위90%.
Strain CH7,isolated from activated sludge,could utilize beta-cypermethrin as the sole carbon resource for growth.Based on the results of physiological-biochemical properties and phylogenetic similarity of 16S rDNA gene sequences,strain CH7 was identified as Pseudomonas aeruginosa. Response surface methodology based on the Box-behnken design of experiment was used to optimize the degrading conditions of strain CH7.Under the optimum degrading conditions(29.4 C,pH7.0,biomass 0.15 g/L),strain CH7 could degrade 90% beta-cypermethrin(100 mg/L)within 12 days.
