CAJ | 학술논문

应用超声破碎碱提法制备自养小球藻多糖.在体外培养条件下,用不同浓度的小球藻多糖处理肝癌细胞,并通过MTT,双苯并咪唑(Hoechst 33258)染色,免疫细胞化学等方法检测.结果表明,1 g/L的小球藻多糖作用肝癌细胞SMMC-7721 12 h,对其增殖有显著抑制作用,且能通过下调抗凋亡蛋白Bcl-2及上调凋亡执行蛋白Capaae-3的表达来诱导其发生凋亡.
응용초성파쇄감제법제비자양소구조다당.재체외배양조건하,용불동농도적소구조다당처리간암세포,병통과MTT,쌍분병미서(Hoechst 33258)염색,면역세포화학등방법검측.결과표명,1 g/L적소구조다당작용간암세포SMMC-7721 12 h,대기증식유현저억제작용,차능통과하조항조망단백Bcl-2급상조조망집행단백Capaae-3적표체래유도기발생조망.
To determine the effects of polysaceharide of Chlorella autotropica (PCA) on the growth and apoptosis of SMMC-7721 cells.The PCA was prepared by ultrasonic disruption and alkali extraction.Human hepatoma ceU culture was treated with three concentrations of PCA,and the activities of PCA were detected by MTT, fluorescence dye Hoechst 33258 staining and cell immunohistochemistry.After treated with PCA(1 g/L) for 12 h,the proliferation of SMMC-7721 was significantly down regulated,and apoptosis was induced.