农业科学与技术（英文版）
農業科學與技術（英文版）
농업과학여기술（영문판）
AGRICULTURAL SCIENCE & TECHNOLOGY
2010年
5期
194-196
,共3页
廖振林%刘菁%陈建宏%钟毓娟%蒋莲秀%陈森洲
廖振林%劉菁%陳建宏%鐘毓娟%蔣蓮秀%陳森洲
료진림%류정%진건굉%종육연%장련수%진삼주
红树林土壤%放线菌%16S%rDNA%链霉菌属
紅樹林土壤%放線菌%16S%rDNA%鏈黴菌屬
홍수림토양%방선균%16S%rDNA%련매균속
Mangrove soil%Actinomycetas%16S rDNA%Streptomyces
[目的]鉴定广西北海红树林土壤放线菌的物种多样性.[方法]从广西北海红树林土壤中分离、筛选10株典型放线菌菌株,提取基因组DNA,进行16S rDNA PCR扩增与测序,并构建进化树.[结果]通过Blast比对,10株放线菌属于2个属,其中8株为链霉菌属(80%),2株为拟诺卡氏菌属(20%).[结论]广西北海红树林土壤蕴减着种类丰富的放线菌.
[目的]鑒定廣西北海紅樹林土壤放線菌的物種多樣性.[方法]從廣西北海紅樹林土壤中分離、篩選10株典型放線菌菌株,提取基因組DNA,進行16S rDNA PCR擴增與測序,併構建進化樹.[結果]通過Blast比對,10株放線菌屬于2箇屬,其中8株為鏈黴菌屬(80%),2株為擬諾卡氏菌屬(20%).[結論]廣西北海紅樹林土壤蘊減著種類豐富的放線菌.
[목적]감정엄서북해홍수림토양방선균적물충다양성.[방법]종엄서북해홍수림토양중분리、사선10주전형방선균균주,제취기인조DNA,진행16S rDNA PCR확증여측서,병구건진화수.[결과]통과Blast비대,10주방선균속우2개속,기중8주위련매균속(80%),2주위의낙잡씨균속(20%).[결론]엄서북해홍수림토양온감착충류봉부적방선균.
[Objective] The aim was to identify the species diversity of Actinomycetes from Mangrove forest in Beihai,Guangxi Province.[Method]10 strains of typical Actinomycetes were isolated from Mangrove forest soil,and the Actinomycetes genomic DNA was successful extracted.16S rDNA was amplified by PCR and sequenced by Sanger dideoxy sequencing method.[Rcsult]All the sequences were blasted in genbank,eight strains belonged to the genus of Streptomyces (80%),and two strains belonged to the genus of Nocardiopsis (20%).[Coacluslon]There are many different Actinomycetes species in Mangrove forest soil samples in Beihai,Guangxi Province.
