CAJ | 학술논문

采用柱层析、制备薄层层析等方法从海洋链霉菌S007的发酵液提取物中分离得到了9个化合物,经波谱分析确定为嘧啶(1),吡咯-2-羧酸(2),卡拉霉素(3),3-吲哚丙酸(4),吲哚-3-羧酸(5),N-乙酰基酪胺(6),2'-胸腺嘧啶脱氧核苷(7),N-β-乙酰色胺酸(8)和三乙胺盐酸盐(9).其中三乙胺盐酸盐是首次从链霉菌中得到;海虾致死实验结果显示:化合物3在40μg/mL浓度下对丰年虾的致死率为86.5%,表明卡拉霉素对丰年虾表现出很强的毒性作用.
채용주층석、제비박층층석등방법종해양련매균S007적발효액제취물중분리득도료9개화합물,경파보분석학정위밀정(1),필각-2-최산(2),잡랍매소(3),3-신타병산(4),신타-3-최산(5),N-을선기락알(6),2'-흉선밀정탈양핵감(7),N-β-을선색알산(8)화삼을알염산염(9).기중삼을알염산염시수차종련매균중득도;해하치사실험결과현시:화합물3재40μg/mL농도하대봉년하적치사솔위86.5%,표명잡랍매소대봉년하표현출흔강적독성작용.
Nine metabolites thymine(1 ), pyrrole-2-carboxylic acid(2 ), kalamycin (3), 3 -indole propionic acid (4),indol-3-carboxylic acid (5), N-acetyl-tyramine (6),2'-deoxythymidine (7), N-ββ-acetyltryptamine (8), and triethylamine hydrochloride (9) were isolated by column and preparative thin layer chromatography from the extract of fermented broth of Streptomycete sp. S007. Their structures were established by NMR and MS data. Among of them,9 was firstly obtained from Streptomycete;The brine shrimp bioassay showed the mortality rates of kalamycin to brine shrimp are 86. 5％ at a concentration of 40 μg/mL,indicating kalamycin possessed significant toxicity on brine shrimp.