CAJ | 학술논문

目的了解大鼠切口疼痛模型中鞘内(IT)新斯的明(NEO)对脊髓一氧化氮合酶(NOS)活性、一氧化氮(NO)产量和环鸟苷酸(cGMP)含量的影响.方法雄性SD大鼠64只,随机分为四组,每组16只:假手术组(组Ⅰ)、术前30min IT 0.9%氯化钠20μl组(组Ⅱ)、术后30min IT NEO 10μg组(组Ⅲ)和术前30min IT NEO 10μg组(组Ⅳ).按Brennan法制成切口疼痛模型,以累积疼痛评分确定疼痛行为.在术后2h断头取腰段脊髓,以分光光度法测定NOS活性、NO产量;放射免疫法测定cGMP含量.结果组Ⅲ和组Ⅳ大鼠的累积评分均明显低于组Ⅱ(P<0.01).组Ⅱ的脊髓NOS活性、NO产量和cGMP含量均较组Ⅰ明显升高(P<0.01).组Ⅳ的脊髓NOS活性、NO产量和cGMP含量均较组Ⅱ明显降低(P<0.05或0.01).而两用药组上述指标比较以及用药组与组Ⅰ比较均无明显差别(P>0.05).结论在大鼠切口疼痛模型中,术前IT NEO的抗伤害作用可能与NO/cGMP信号转导系统有关.
목적료해대서절구동통모형중초내(IT)신사적명(NEO)대척수일양화담합매(NOS)활성、일양화담(NO)산량화배조감산(cGMP)함량적영향.방법웅성SD대서64지,수궤분위사조,매조16지:가수술조(조Ⅰ)、술전30min IT 0.9%록화납20μl조(조Ⅱ)、술후30min IT NEO 10μg조(조Ⅲ)화술전30min IT NEO 10μg조(조Ⅳ).안Brennan법제성절구동통모형,이루적동통평분학정동통행위.재술후2h단두취요단척수,이분광광도법측정NOS활성、NO산량;방사면역법측정cGMP함량.결과조Ⅲ화조Ⅳ대서적루적평분균명현저우조Ⅱ(P<0.01).조Ⅱ적척수NOS활성、NO산량화cGMP함량균교조Ⅰ명현승고(P<0.01).조Ⅳ적척수NOS활성、NO산량화cGMP함량균교조Ⅱ명현강저(P<0.05혹0.01).이량용약조상술지표비교이급용약조여조Ⅰ비교균무명현차별(P>0.05).결론재대서절구동통모형중,술전IT NEO적항상해작용가능여NO/cGMP신호전도계통유관.
Objective To investigate the effects of intrathecal neostigmine on nitric oxide synthase (NOS) activity, nitric oxide (NO) production and cyclic guanosine 3 ', 5 '- monophosphate (cGMP)content of the spinal cord in a rat model of incisional pain. Methods Male SD rats weighing 250-300g were anesthetized with intraperitoneal pentobarbital sodium 40 mg@kg-1 . Intrathecal(IT) catheter was implanted and the tip of the cathter reached the lumbar region, according to the method of Yaksh. Incision was made in the plantar aspect of the left hindpaw under 1.4% isoflurane anesthesia, according to the method of Brennan. 64 male SD rats were were divided randomly into four groups. Group Ⅰ received IT 0.9% NaCl 20 μl and 30 min later inhaled 1.4% isoflurane for 5 min but no incision was made. Group Ⅱ received IT 0.9% NaC1 20 μl 30 min before incision. Group Ⅲ received IT neostigmine 10μg(10μl) 30 min after incision. GroupⅣ received IT neostigmine 10μg(10μl) 30 min before incision. In group Ⅲ and Ⅳ 0.9 % NaC1 10 μl was flushed through IT catheter after IT neostigmine administration. A cumulative pain score was utilized to assess pain behavior. The animals were decapitated 2h after incision and the spinal cord was immediately removed and lumbar enlargement of the spinal cord was dissected on ice. NOS activity, NO production and cGMP content were measured by spectrophotometry and radioimmunoassy. Results Cumulative pain score in group Ⅲ and group Ⅳ was significantly lower than that in group Ⅱ (P<0.01 ).NOS activity, NO production as well as cGMP content of the spinal cord increased significantly in group Ⅱ as compared with those in group Ⅰ (P<0.01). NOS activity, NO production and cGMP content of the spinal cord in group Ⅳ were significantly lower than those in group Ⅱ (P<0.05 or P < 0.01 ). In group Ⅲ NOS activity, NO production and cGMP content of the spinal cord tended to be lower than those in group Ⅱ, although the differences were not statistically significant. Conclusions In a rat model of incisional pain antinociceptive effect of IT administration of neostigmine may be associated with the NO/cGMP signaling pathway of the spinal cord.