中华儿科杂志
中華兒科雜誌
중화인과잡지
Chinese Journal of Pediatrics
2008年
2期
111-114
,共4页
赵林清%钱渊%朱汝南%邓洁%王芳%董慧瑾%LI Yan
趙林清%錢淵%硃汝南%鄧潔%王芳%董慧瑾%LI Yan
조림청%전연%주여남%산길%왕방%동혜근%LI Yan
细小病毒科%病毒结构蛋白质类%免疫球蛋白G%印迹法,蛋白质
細小病毒科%病毒結構蛋白質類%免疫毬蛋白G%印跡法,蛋白質
세소병독과%병독결구단백질류%면역구단백G%인적법,단백질
Parvoviridae%Viral structural proteins%Immunoglobulin G%Blotting,western
目的 通过对血清中人Boca病毒(HBoV)主要衣壳蛋白VP2特异性IgG抗体进行检测,初步了解北京地区人群中这种新发现的病毒的感染状况.方法 以大肠杆菌表达的HBoV主要衣壳蛋白VP2为抗原,应用Western-blot方法,对1996年4月至1997年3月取自首都儿科研究所附属儿童医院健康查体者及北京宣武医院非呼吸道感染患者的血清标本共677份进行HBoV VP2蛋白特异性IgG抗体检测.设抗组氨酸抗体及兔抗HBoV-VP2多肽特异性免疫血清为阳性血清对照.结果 (1)677份血清标本中,抗HBoV VP2蛋白的IgG抗体阳性400份,总检出率为59.1%.(2)被检对象中,＜1个月的婴儿抗体阳性率为45.3%,1个月～的婴儿抗体阳性率为41.4%,2个月～的婴儿抗体阳性率最低(31.3%),6个月～至7岁龄抗体阳性检出率在45.6%～69.7%,7岁后直至40岁,抗体阳性检出率维持在70%左右;50岁后则为61.8%～62.8%.结论 早在1996年北京地区的人群中就有59.1%曾经感染过HBoV,说明这种病毒是一种新发现的病毒而不是新出现的病毒,北京地区人群中该病毒的感染较常见.6个月龄以前的婴儿为易感人群.
目的 通過對血清中人Boca病毒(HBoV)主要衣殼蛋白VP2特異性IgG抗體進行檢測,初步瞭解北京地區人群中這種新髮現的病毒的感染狀況.方法 以大腸桿菌錶達的HBoV主要衣殼蛋白VP2為抗原,應用Western-blot方法,對1996年4月至1997年3月取自首都兒科研究所附屬兒童醫院健康查體者及北京宣武醫院非呼吸道感染患者的血清標本共677份進行HBoV VP2蛋白特異性IgG抗體檢測.設抗組氨痠抗體及兔抗HBoV-VP2多肽特異性免疫血清為暘性血清對照.結果 (1)677份血清標本中,抗HBoV VP2蛋白的IgG抗體暘性400份,總檢齣率為59.1%.(2)被檢對象中,＜1箇月的嬰兒抗體暘性率為45.3%,1箇月～的嬰兒抗體暘性率為41.4%,2箇月～的嬰兒抗體暘性率最低(31.3%),6箇月～至7歲齡抗體暘性檢齣率在45.6%～69.7%,7歲後直至40歲,抗體暘性檢齣率維持在70%左右;50歲後則為61.8%～62.8%.結論 早在1996年北京地區的人群中就有59.1%曾經感染過HBoV,說明這種病毒是一種新髮現的病毒而不是新齣現的病毒,北京地區人群中該病毒的感染較常見.6箇月齡以前的嬰兒為易感人群.
목적 통과대혈청중인Boca병독(HBoV)주요의각단백VP2특이성IgG항체진행검측,초보료해북경지구인군중저충신발현적병독적감염상황.방법 이대장간균표체적HBoV주요의각단백VP2위항원,응용Western-blot방법,대1996년4월지1997년3월취자수도인과연구소부속인동의원건강사체자급북경선무의원비호흡도감염환자적혈청표본공677빈진행HBoV VP2단백특이성IgG항체검측.설항조안산항체급토항HBoV-VP2다태특이성면역혈청위양성혈청대조.결과 (1)677빈혈청표본중,항HBoV VP2단백적IgG항체양성400빈,총검출솔위59.1%.(2)피검대상중,＜1개월적영인항체양성솔위45.3%,1개월～적영인항체양성솔위41.4%,2개월～적영인항체양성솔최저(31.3%),6개월～지7세령항체양성검출솔재45.6%～69.7%,7세후직지40세,항체양성검출솔유지재70%좌우;50세후칙위61.8%～62.8%.결론 조재1996년북경지구적인군중취유59.1%증경감염과HBoV,설명저충병독시일충신발현적병독이불시신출현적병독,북경지구인군중해병독적감염교상견.6개월령이전적영인위역감인군.
Objective To find out the importance of human bocavirus(HBoV)as an infectious agent for population in Beijing,China,seroprevalence study was conducted by using expressed recombinant major capsid VP2 protein as an antigen.Methods Serum specimens collected from infants and children who visited the Children's Hospital Affiliated to the Capital Institute of Pediatrics for health check up and adults visited the Xuanwu Hospital,Beijing for diseases other than respiratory infections from April 1996 to March 1997 were used for investigation.The major capsid protein VP2 from HBoV was expressed in E.coli strain BL21(DE3)with the transformed PET30b vector inserted with full-length VP2 gene of HBo V and the specific antigenicity of this expressed protein was validated by previous study.Western blot was used to detect specific IgG antibody against HBoV in collected serum specimens diluted to 1:200.Mock expressed protein was E.coli cells strain B121(DE3)with the transformed PET30b vector without insert.Anti-His monoclonal antibody and rabbit anti-HBoV VP2 polypeptides hyper-immune serum were used as positive control for antibody detection.Results Out of 677 serum specimens tested,400(59.1%)were positive by Western blot.About 45.3%(34/75)of the newborns under 1 month of age had anti-HBoV antibodies,and antibody positive rates were lower in the age groups of 1 and 2 months(41.4% and 31.3%,respectively)and were higher in the following ages from 6 months to 7 years(from 45.6% to 69.7%).The antibody positive rates were at a relatively constant level(about 70%)in the age groups from 7 years to 40 years and became lower(61.8%-62.8%)in groups of age over 50 years.Conclusion The high seroprevalence against recombinant HBoV VP2 protein and early age antibody acquisition indicate that HBoV has been circulating in Beijing,China as early as in 1996 and most of children had been exposed to HBoV by the age of 7 years.Infants under the age of 6 months were susceptible to infection with this virus.
