CAJ | 학술논문

目的研究60Co γ线不同剂量照射条件培养液(ICM)培养k562细胞后引发的旁效应.方法采用健康男性外周血自然杀伤(NK)细胞.观察不同剂量60Coγ线照射的条件培养液培养后引发的K562细胞对NK细胞敏感性的改变,同时用流式细胞仪检测K552细胞的凋亡情况.结果照射组NK细胞活性显著增加,即受照肿瘤细胞的培养基显著影响了未受照肿瘤细胞的存活能力,从而NK细胞对未照射细胞的活性显著增加;其中,从0.5 Gy起即出现显著升高,1.0 Gy继续增加,2.0和5.0 Gy出现波动,在8.5 Gy达到最高.照射组K<562>细胞的凋亡率比对照组显著增加(P<0.01),即受照肿瘤细胞的培养基显著影响了未受照肿瘤细胞的存活能力.在0.5 Gy处,即出现非常明显的效应.结论对于K562细胞,ICM对细胞显示出明显损伤作用,NK细胞活性显著增加,凋亡率升高.提示存在培养基转移介导的电离辐射旁效应.
목적 연구60Co γ선불동제량조사조건배양액(ICM)배양k562세포후인발적방효응.방법 채용건강남성외주혈자연살상(NK)세포.관찰불동제량60Coγ선조사적조건배양액배양후인발적K562세포대NK세포민감성적개변,동시용류식세포의검측K552세포적조망정황.결과 조사조NK세포활성현저증가,즉수조종류세포적배양기현저영향료미수조종류세포적존활능력,종이NK세포대미조사세포적활성현저증가;기중,종0.5 Gy기즉출현현저승고,1.0 Gy계속증가,2.0화5.0 Gy출현파동,재8.5 Gy체도최고.조사조K<562>세포적조망솔비대조조현저증가(P<0.01),즉수조종류세포적배양기현저영향료미수조종류세포적존활능력.재0.5 Gy처,즉출현비상명현적효응.결론 대우K562세포,ICM대세포현시출명현손상작용,NK세포활성현저증가,조망솔승고.제시존재배양기전이개도적전리복사방효응.
Objective To study the bystander effects and associated mechanisms through irradiated conditioned medium(1CM). Methods Natural kilhr(NK) cells were obtained from peripheral blood samples. ICM irradiated with different doses of 60Coγ-rays was used for culturing K562 cell strain. The degree of injury of K562cells by activated NK cells was observed, as well as the apoptosis frequency of K562 cell was investigated. Results Severe injury was induced in K562 cells cultured in ICM than the control (sham-irradiated) as shown by increased sensitivity to NK cells (P < 0.05). The apoptosis frequency of K562 cell was increased significantly compared with the control cells (P < 0.05). Conclusions The bystander effect induced by irradiation is existent. ICM can trigger the bystander effect on K562 cell strains.