中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2011年
6期
352-356
,共5页
张国翠%王宏萍%胡芸文%周晓明%鲍依稀
張國翠%王宏萍%鬍蕓文%週曉明%鮑依稀
장국취%왕굉평%호예문%주효명%포의희
呼吸道感染%呼吸道合胞体病毒,人%鼻病毒属%腺病毒科%基因扩增%DNA,环状%重叠感染
呼吸道感染%呼吸道閤胞體病毒,人%鼻病毒屬%腺病毒科%基因擴增%DNA,環狀%重疊感染
호흡도감염%호흡도합포체병독,인%비병독속%선병독과%기인확증%DNA,배상%중첩감염
Respiratory Tract Infections%Respiratory Syncytial Virus,Human%Rhinovirus%Adenoviridae%Gene Amplification%DNA,Circular%Superinfection
目的 使用Seeplex(R)RV15 ACE检测试剂盒检测上海地区婴儿呼吸道感染病毒的病原谱系.方法 征集新华医院儿科呼吸道发热门诊0～1岁组患儿62例,以咽拭子对呼吸道分泌物采样,提取样本中病毒核酸,反转录合成cDNA,使用Seeplex(R)RV15 ACE检测试剂盒扩增15种呼吸道病毒的目的 基因,以琼脂糖凝胶电泳检测特异性产物条带,判定感染病毒.并以检出病毒与检出季节和临床特征进行比较.数据采用Fisher's精确检验和卡方检验.结果 62例呼吸道感染患儿从咽拭子中检出病毒阳性36例,占58.1%,其中呼吸道合胞病毒B型(RSV-B)、鼻病毒、腺病毒、肠道病毒(hEV)、冠状病毒229E-NL63、A型流感病毒、副流感病毒1型和3型、博卡病毒、偏肺病毒、B型流感病毒的检出率分别为12.9%、11.3%、11.3%、8.0%、6.5%、6.5%、4.8%、4.8%、1.6%、1.6%和1.6%.检出双重感染6例,三重感染1例,多重感染占阳性病例的19.4%(7/36).冬季RSV-B检出率,高于其他三季(Fisher's精确检验,P=0.001),夏季检出hEV高于其他三季(Fisher's精确检验,P=0.01).结论 RSV-B、鼻病毒和腺病毒是上海地区婴儿呼吸道感染的前三位病毒病原体,多重病毒感染常见.
目的 使用Seeplex(R)RV15 ACE檢測試劑盒檢測上海地區嬰兒呼吸道感染病毒的病原譜繫.方法 徵集新華醫院兒科呼吸道髮熱門診0～1歲組患兒62例,以嚥拭子對呼吸道分泌物採樣,提取樣本中病毒覈痠,反轉錄閤成cDNA,使用Seeplex(R)RV15 ACE檢測試劑盒擴增15種呼吸道病毒的目的 基因,以瓊脂糖凝膠電泳檢測特異性產物條帶,判定感染病毒.併以檢齣病毒與檢齣季節和臨床特徵進行比較.數據採用Fisher's精確檢驗和卡方檢驗.結果 62例呼吸道感染患兒從嚥拭子中檢齣病毒暘性36例,佔58.1%,其中呼吸道閤胞病毒B型(RSV-B)、鼻病毒、腺病毒、腸道病毒(hEV)、冠狀病毒229E-NL63、A型流感病毒、副流感病毒1型和3型、博卡病毒、偏肺病毒、B型流感病毒的檢齣率分彆為12.9%、11.3%、11.3%、8.0%、6.5%、6.5%、4.8%、4.8%、1.6%、1.6%和1.6%.檢齣雙重感染6例,三重感染1例,多重感染佔暘性病例的19.4%(7/36).鼕季RSV-B檢齣率,高于其他三季(Fisher's精確檢驗,P=0.001),夏季檢齣hEV高于其他三季(Fisher's精確檢驗,P=0.01).結論 RSV-B、鼻病毒和腺病毒是上海地區嬰兒呼吸道感染的前三位病毒病原體,多重病毒感染常見.
목적 사용Seeplex(R)RV15 ACE검측시제합검측상해지구영인호흡도감염병독적병원보계.방법 정집신화의원인과호흡도발열문진0～1세조환인62례,이인식자대호흡도분비물채양,제취양본중병독핵산,반전록합성cDNA,사용Seeplex(R)RV15 ACE검측시제합확증15충호흡도병독적목적 기인,이경지당응효전영검측특이성산물조대,판정감염병독.병이검출병독여검출계절화림상특정진행비교.수거채용Fisher's정학검험화잡방검험.결과 62례호흡도감염환인종인식자중검출병독양성36례,점58.1%,기중호흡도합포병독B형(RSV-B)、비병독、선병독、장도병독(hEV)、관상병독229E-NL63、A형류감병독、부류감병독1형화3형、박잡병독、편폐병독、B형류감병독적검출솔분별위12.9%、11.3%、11.3%、8.0%、6.5%、6.5%、4.8%、4.8%、1.6%、1.6%화1.6%.검출쌍중감염6례,삼중감염1례,다중감염점양성병례적19.4%(7/36).동계RSV-B검출솔,고우기타삼계(Fisher's정학검험,P=0.001),하계검출hEV고우기타삼계(Fisher's정학검험,P=0.01).결론 RSV-B、비병독화선병독시상해지구영인호흡도감염적전삼위병독병원체,다중병독감염상견.
Objective To investigate the etiology spectrum of respiratory viral infection in the infants in Shanghai by using Seeplex(R) RV15 ACE Detection kit. Methods Sixty-two throat swab samples were collected from the infant patients under one-year age, who were admitted to pediatric clinic in Xinhua Hospital during June, 2009 to June, 2010. Among these samples, 15 species of respiratory virus were amplified by using Seeplex(R) RV15 ACE Detection kit. Identified viruses were analyzed for their seasonal distribution pattern and clinical infectious symptoms. The data were analyzed by Fisher's exact test and chi square test. Results Viruses were successfully detected from 36 swab samples (58. 1％). Among them, respiratory syncytial virus-B (RSV-B), human rhinovirus (hRV) and adenovirus (ADV) accounted for 12. 9％, 11. 3％ and 11. 3％ of the infections, respectively. Other detected viruses included human enterovirus (hEV) (8.0％), influenza virus A (FluA) and coronavirus 229E-NL63 (each 6. 5％), parainfluenzavirus (PIV)-1 and PIV-3 (each 4. 8％), human Boca virus (HBoV), human metapneunovirus (hMPV) and influenza virus B (FluB) (each 1. 6％). Dual infections were identified in six cases and triple infections in one case, which as a whole accounted for 19. 4％ of positive cases (7/36). RSV-B infection in winter was significantly
higher than the other three seasons. Of the detection rate (Fisher's exact test, P = 0. 001), hEV infection in summer was significantly higher than the other three quarters (Fisher's exact test, P = 0.01). Conclusions RSV-B, hRV, and ADV are the three most common etiologies in respiratory viral infection in the infants under one-year age in Shanghai area. Multiple infections are also common.
