中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2009年
4期
581-582
,共2页
海蛞蝓属%肝炎病毒,乙型,鸭%疾病模型,动物
海蛞蝓屬%肝炎病毒,乙型,鴨%疾病模型,動物
해활유속%간염병독,을형,압%질병모형,동물
Hermissends%Hepatitis B virus,uuck%Disease models,animal
目的 研究蛞蝓多糖抗鸭乙型肝炎病毒(DHBV)的作用.方法 采用先天感染DHBV的北京雏鸭为模型,随机分设0.9%氯化钠注射液模型对照组、拉米夫定组、蛞蝓多糖低、中、高3个剂量组,每组8只雏鸭均以20 mg·kg-1·d-1灌胃给药10 d.斑点杂交法观察用药前与用药后5 d、10 d及停药后5 d血清中DHBV-DNA表达.结果 蛞蝓多糖不同剂量组对DHBV均有一定抑制作用.其中蛞蝓多糖的中、高剂量组于用药后第5天血清DHBV-DNA的吸光度A值为(0.66±0.14)、(0.73±0.26),第10天为(0.47±0.18)、(0.54±0.10),较0.9%氯化钠注射液对照组(1.61±0.54)、(1.20±0.51)明显降低.蛞蝓多糖的中、高剂量组对DHBV-DNA的抑制率于用药后第5天为24.14%、23.96%,第10天为45.98%、43.75%,与0.9%氯化钠注射液对照组相比明显提高.结论 蛞蝓多糖具有降低血DHBV-DNA含量,抑制乙型肝炎病毒作用.
目的 研究蛞蝓多糖抗鴨乙型肝炎病毒(DHBV)的作用.方法 採用先天感染DHBV的北京雛鴨為模型,隨機分設0.9%氯化鈉註射液模型對照組、拉米伕定組、蛞蝓多糖低、中、高3箇劑量組,每組8隻雛鴨均以20 mg·kg-1·d-1灌胃給藥10 d.斑點雜交法觀察用藥前與用藥後5 d、10 d及停藥後5 d血清中DHBV-DNA錶達.結果 蛞蝓多糖不同劑量組對DHBV均有一定抑製作用.其中蛞蝓多糖的中、高劑量組于用藥後第5天血清DHBV-DNA的吸光度A值為(0.66±0.14)、(0.73±0.26),第10天為(0.47±0.18)、(0.54±0.10),較0.9%氯化鈉註射液對照組(1.61±0.54)、(1.20±0.51)明顯降低.蛞蝓多糖的中、高劑量組對DHBV-DNA的抑製率于用藥後第5天為24.14%、23.96%,第10天為45.98%、43.75%,與0.9%氯化鈉註射液對照組相比明顯提高.結論 蛞蝓多糖具有降低血DHBV-DNA含量,抑製乙型肝炎病毒作用.
목적 연구활유다당항압을형간염병독(DHBV)적작용.방법 채용선천감염DHBV적북경추압위모형,수궤분설0.9%록화납주사액모형대조조、랍미부정조、활유다당저、중、고3개제량조,매조8지추압균이20 mg·kg-1·d-1관위급약10 d.반점잡교법관찰용약전여용약후5 d、10 d급정약후5 d혈청중DHBV-DNA표체.결과 활유다당불동제량조대DHBV균유일정억제작용.기중활유다당적중、고제량조우용약후제5천혈청DHBV-DNA적흡광도A치위(0.66±0.14)、(0.73±0.26),제10천위(0.47±0.18)、(0.54±0.10),교0.9%록화납주사액대조조(1.61±0.54)、(1.20±0.51)명현강저.활유다당적중、고제량조대DHBV-DNA적억제솔우용약후제5천위24.14%、23.96%,제10천위45.98%、43.75%,여0.9%록화납주사액대조조상비명현제고.결론 활유다당구유강저혈DHBV-DNA함량,억제을형간염병독작용.
Objective To study the anti-duck hepatitis B virus (DHBV) action of limax polysaccharide. Methods 1-day old Beijing ducklings with congenital infection of DHBV were used as the animal model and were days. The expression of DHBV-DNA was detected by dot-blot hybridization test before treatment, five days and ten days after treatment. Results After medicated with Limax polysaccharide for five days and for ten days. The optic density value(OD value) of DHBV-DNA in ducklings serum were obviusly lower than that before treatment. There was significant difference between them. The inhibition rote of limax polysacchatide compared with the model group is markedly improved. Conclusion Limax polysaccharide can protect duck liver against inflammation by the inhibition of DHBV.
