中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2009年
5期
297-300
,共4页
王浙君%撒悦%马霄%王贻宁%蒋滔
王浙君%撒悅%馬霄%王貽寧%蔣滔
왕절군%살열%마소%왕이저%장도
羟基磷厌石类%牙本质敏感%酪蛋白磷酸肽钙磷复合体
羥基燐厭石類%牙本質敏感%酪蛋白燐痠肽鈣燐複閤體
간기린염석류%아본질민감%락단백린산태개린복합체
Hydroxyapatites%Dentin sensitivity%Casein phosphopeptide-amorphous calcium phosphate
目的 通过合成纳米羟基磷灰石(hydroxyapatite,HA)以及体外实验观察其对牙本质小管的封闭效果,为纳米HA的临床应用提供依据.方法 采用磷酸氢二铵[(NH4)2HPO4]和硝酸钙[Ca(NO3)2]合成纳米HA,扫描电子显微镜(scanning electron microscope,SEM)、透射电子显微镜(transmission electron microscope,TEM)、傅里叶变换红外光谱计(Fourier transform infrared spectrometer,FTIR)和X射线衍射仪(X-ray diffractometer,XRD)检测纳米HA.选取8颗健康第三磨牙制备24片牙本质薄片,按随机数字表分为酪蛋白磷酸肽钙磷复合体(casein phosphopeptideamorphous calcium phosphate,CPP-ACP)组、纳米HA组及空白对照组,每组8片;空白对照组不做处理,CPP-ACP组和纳米HA组每天分别用CPP-ACP和纳米HA处理牙本质薄片2次,7d后SEM观察牙本质表面.结果 经SEM、TEM、FTIR和XRD检测,证实本项实验制备的HA为纳米HA;SEM观察纳米HA对牙本质小管的封闭效果明显高于CPP-ACP组和窄白对照组.结论 在体外条件下,与CPP-ACP相比,纳米HA可更有效地封闭暴露的牙本质小管.
目的 通過閤成納米羥基燐灰石(hydroxyapatite,HA)以及體外實驗觀察其對牙本質小管的封閉效果,為納米HA的臨床應用提供依據.方法 採用燐痠氫二銨[(NH4)2HPO4]和硝痠鈣[Ca(NO3)2]閤成納米HA,掃描電子顯微鏡(scanning electron microscope,SEM)、透射電子顯微鏡(transmission electron microscope,TEM)、傅裏葉變換紅外光譜計(Fourier transform infrared spectrometer,FTIR)和X射線衍射儀(X-ray diffractometer,XRD)檢測納米HA.選取8顆健康第三磨牙製備24片牙本質薄片,按隨機數字錶分為酪蛋白燐痠肽鈣燐複閤體(casein phosphopeptideamorphous calcium phosphate,CPP-ACP)組、納米HA組及空白對照組,每組8片;空白對照組不做處理,CPP-ACP組和納米HA組每天分彆用CPP-ACP和納米HA處理牙本質薄片2次,7d後SEM觀察牙本質錶麵.結果 經SEM、TEM、FTIR和XRD檢測,證實本項實驗製備的HA為納米HA;SEM觀察納米HA對牙本質小管的封閉效果明顯高于CPP-ACP組和窄白對照組.結論 在體外條件下,與CPP-ACP相比,納米HA可更有效地封閉暴露的牙本質小管.
목적 통과합성납미간기린회석(hydroxyapatite,HA)이급체외실험관찰기대아본질소관적봉폐효과,위납미HA적림상응용제공의거.방법 채용린산경이안[(NH4)2HPO4]화초산개[Ca(NO3)2]합성납미HA,소묘전자현미경(scanning electron microscope,SEM)、투사전자현미경(transmission electron microscope,TEM)、부리협변환홍외광보계(Fourier transform infrared spectrometer,FTIR)화X사선연사의(X-ray diffractometer,XRD)검측납미HA.선취8과건강제삼마아제비24편아본질박편,안수궤수자표분위락단백린산태개린복합체(casein phosphopeptideamorphous calcium phosphate,CPP-ACP)조、납미HA조급공백대조조,매조8편;공백대조조불주처리,CPP-ACP조화납미HA조매천분별용CPP-ACP화납미HA처리아본질박편2차,7d후SEM관찰아본질표면.결과 경SEM、TEM、FTIR화XRD검측,증실본항실험제비적HA위납미HA;SEM관찰납미HA대아본질소관적봉폐효과명현고우CPP-ACP조화착백대조조.결론 재체외조건하,여CPP-ACP상비,납미HA가경유효지봉폐폭로적아본질소관.
Objective To synthesize nano-hydroxyapatite(nano-HA)and evaluate its effect on the occlusion of dentinal tubules in vitro,and therefore provide evidence for the clinical application.Methods (NH4)2 HPO4 and Ca(NO3)2 were used to form nano-HA,which was characterized by scanning electron microscopy(SEM),transmission electron microscopy(TEM),Fourier transform infrared spectrometer (FTIR)and X-ray diffractometer(XRD)respectively.Twenty-four dentin slabs were obtained from 8 healthy third molars and randomly divided into 3 groups,which were control group,casein phosphopetide-amorphous calcium phosphate(CPP-ACP)group,and nano-HA group.After the CPP-ACP and nano-HA were topically applied to the slabs of two study groups twice a day for 7 days,the surface of slab dentin Was observed using SEM.Results SEM,TEM,FTIR,and XRD tests showed that nano-HA was synthesized successfully.SEM observations revealed that the sealing of dentinal tubules of nano-HA group was extremely high when compared with those of control and CPP-ACP group.Conclusions In comparison with CPP-ACP.nano-HA could occlude dentinal tubules more effectively in vitro.
