中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2008年
5期
471-473,478
,共4页
范学政%姜军合%翟安林%李俊%王帆
範學政%薑軍閤%翟安林%李俊%王帆
범학정%강군합%적안림%리준%왕범
脑挫裂伤%白细胞介素-1受体拮抗剂%白细胞介素-1β%S-100β蛋白
腦挫裂傷%白細胞介素-1受體拮抗劑%白細胞介素-1β%S-100β蛋白
뇌좌렬상%백세포개소-1수체길항제%백세포개소-1β%S-100β단백
Cerebral contusion and laceration%IL-1ra%IL-1β%S-100β protein
目的 研究白细胞介素-1受体拮抗剂(IL-1ra)对大鼠脑挫裂伤后血浆S-100β蛋白含量变化和大鼠神经功能行为评分的影响,探讨IL-1ra脑保护作用的可能机制.方法 雄性Wistar大鼠采用随机数字表法分为生理盐水组(n=30)及IL-1ra组(n=30),分别经侧脑室注射生理盐水、IL-1ra 5 μL,并设立正常对照组(n=5).30 min后按Feeney法将生理盐水组及IL-1ra组大鼠制作出脑挫裂伤模型,正常对照组不做任何处理.采用酶联免疫吸附试验(ELISA)测定脑挫裂伤后6 h、12 h、24 h、2 d、3 d、7 d各组大鼠血浆S-100β蛋白含量,Faden评分标准评定大鼠颅脑创伤后各时间点神经功能行为.结果 (1)正常对照组大鼠血浆S-100β蛋白含量为(0.43±0.04)μg/L,神经功能评分为35分.(21伤后各时间点生理盐水组及IL-1ra组大鼠血清S-100β蛋白含量显著高于正常对照组(P<0.05).(3)IL-1ra组各时间点大鼠血清S-100β蛋白含量低于生理盐水组,且差异有统计学意义(P<0.05).(4)IL-1ra组伤后各时间点神经功能评分显著高于生理盐水组(P<0.05).结论 (1)IL-1ra可降低脑挫裂伤大鼠血浆S-100β蛋白含量,提高大鼠神经功能评分.(2)IL-1ra对脑挫裂伤大鼠具有脑保护作用,可能与IL-1ra阻断IL-1β介导的损伤性脑细胞炎症反应有关.
目的 研究白細胞介素-1受體拮抗劑(IL-1ra)對大鼠腦挫裂傷後血漿S-100β蛋白含量變化和大鼠神經功能行為評分的影響,探討IL-1ra腦保護作用的可能機製.方法 雄性Wistar大鼠採用隨機數字錶法分為生理鹽水組(n=30)及IL-1ra組(n=30),分彆經側腦室註射生理鹽水、IL-1ra 5 μL,併設立正常對照組(n=5).30 min後按Feeney法將生理鹽水組及IL-1ra組大鼠製作齣腦挫裂傷模型,正常對照組不做任何處理.採用酶聯免疫吸附試驗(ELISA)測定腦挫裂傷後6 h、12 h、24 h、2 d、3 d、7 d各組大鼠血漿S-100β蛋白含量,Faden評分標準評定大鼠顱腦創傷後各時間點神經功能行為.結果 (1)正常對照組大鼠血漿S-100β蛋白含量為(0.43±0.04)μg/L,神經功能評分為35分.(21傷後各時間點生理鹽水組及IL-1ra組大鼠血清S-100β蛋白含量顯著高于正常對照組(P<0.05).(3)IL-1ra組各時間點大鼠血清S-100β蛋白含量低于生理鹽水組,且差異有統計學意義(P<0.05).(4)IL-1ra組傷後各時間點神經功能評分顯著高于生理鹽水組(P<0.05).結論 (1)IL-1ra可降低腦挫裂傷大鼠血漿S-100β蛋白含量,提高大鼠神經功能評分.(2)IL-1ra對腦挫裂傷大鼠具有腦保護作用,可能與IL-1ra阻斷IL-1β介導的損傷性腦細胞炎癥反應有關.
목적 연구백세포개소-1수체길항제(IL-1ra)대대서뇌좌렬상후혈장S-100β단백함량변화화대서신경공능행위평분적영향,탐토IL-1ra뇌보호작용적가능궤제.방법 웅성Wistar대서채용수궤수자표법분위생리염수조(n=30)급IL-1ra조(n=30),분별경측뇌실주사생리염수、IL-1ra 5 μL,병설립정상대조조(n=5).30 min후안Feeney법장생리염수조급IL-1ra조대서제작출뇌좌렬상모형,정상대조조불주임하처리.채용매련면역흡부시험(ELISA)측정뇌좌렬상후6 h、12 h、24 h、2 d、3 d、7 d각조대서혈장S-100β단백함량,Faden평분표준평정대서로뇌창상후각시간점신경공능행위.결과 (1)정상대조조대서혈장S-100β단백함량위(0.43±0.04)μg/L,신경공능평분위35분.(21상후각시간점생리염수조급IL-1ra조대서혈청S-100β단백함량현저고우정상대조조(P<0.05).(3)IL-1ra조각시간점대서혈청S-100β단백함량저우생리염수조,차차이유통계학의의(P<0.05).(4)IL-1ra조상후각시간점신경공능평분현저고우생리염수조(P<0.05).결론 (1)IL-1ra가강저뇌좌렬상대서혈장S-100β단백함량,제고대서신경공능평분.(2)IL-1ra대뇌좌렬상대서구유뇌보호작용,가능여IL-1ra조단IL-1β개도적손상성뇌세포염증반응유관.
Objective To investigate the effect of IL- 1ra on S- 100β protein contents of serum and the behavior scales of neural function in rats with cerebral contusion and laceration by injecting IL-1ra into their later ventricle, and to explore the possible mechanism underlying the nerve protection of IL-1ra. Methods Male Wistar rats were randomly divided into 3 groups:normal saline group(NS group, NS 5 μL injected into the left cerebral ventricle), IL-1ra group (IL-1ra 5 μg/5 μL injected into the left cerebral ventricle), and control group. After half an hour, cerebral contusion and laceration model was made according to Feeney's method in rats of NS group and IL-1ra group. The serum levels of S-100β protein in rats of each group were measured by ELISA at 6 h,12 h,24 h,2 d,3 d,7 d after hitting; and the behavior scales of neural function in all rats were evaluated based on Faden's method.Results (1)The level of serum S-100β protein was (0.43±0.04) μg/L, the score of neural function was 35 in rats of control group.(2)All the model rats' serum concentration of S-100β was higher than that of the control group markedly (P＜0.05) after injury.(3)S-100β concentration of rats in IL-1ra group was significantly lower than that of NS group at the each time point after injury (P＜0.05), and the scores of neural function were higher than those of NS group (P＜0.05). Conclusions (1)IL-1ra can decrease the level of serum S-100β protein and raise the behavior scores of neural function in rats. (2)IL-1ra has brain protective effect after cerebral injury, which may be related to the decrease of the inflammatory reaction of brain cell mediated by IL-1β.
