中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2011年
1期
57-61
,共5页
蒲双双%闫玉芬%高文花%温红玲%王志玉%宋艳艳%许洪芝%赵丽
蒲雙雙%閆玉芬%高文花%溫紅玲%王誌玉%宋豔豔%許洪芝%趙麗
포쌍쌍%염옥분%고문화%온홍령%왕지옥%송염염%허홍지%조려
人类免疫缺陷病毒1型(HIV-1)%HIV-1 tat%艾滋病痴呆综合征(ADC)%基因多态性%氨基酸序列
人類免疫缺陷病毒1型(HIV-1)%HIV-1 tat%艾滋病癡呆綜閤徵(ADC)%基因多態性%氨基痠序列
인류면역결함병독1형(HIV-1)%HIV-1 tat%애자병치태종합정(ADC)%기인다태성%안기산서렬
Human immunodeficiency virus 1 ( HIV-1 )%HIV-1 tat%AIDS dementia complex (ADC)%Genetic diversity%Amino acid sequence
目的 研究艾滋病痴呆综合征患者体内HIV-1 tat第一外显子基因序列的特征和变异情况,为艾滋病痴呆综合征发病机制的研究提供依据.方法 提取1例艾滋病痴呆综合征病例尸检标本的外周组织(淋巴结、脾脏)和中枢神经组织(脑膜、额叶灰质、额叶白质、颞叶、基底核)共7个部位的基因组DNA,巢式PCR扩增HIV-1 tat第一外显子基因,与克隆载体pGEM-T连接,经转化、氨苄青霉素和蓝白斑筛选出阳性克隆,每个部位挑取5个菌落测序,测序结果利用BioEdit、MEGA4软件比对并生成系统进化树、计算基因距离和同义/非同义替换值(ds/dn),分析氨基酸位点的改变.结果 该病例感染的病毒是HIV-1 B亚型,分离自不同组织的HIV-1 tat第一外显子基因序列存在差异;与标准序列相比,该病例的HIV-1tat第一外显子基因编码的氨基酸序列有16个位点发生了变异,并且中枢各部位部分氨基酸位点的变异与外周不同,特别是中枢基底核5个序列及颞叶1个序列Q54R的变异值得关注.结论 艾滋病痴呆综合征患者体内,HIV-1 tat第一外显子序列与标准序列存在差异,并且在外周和中枢不同部位中存在的变异不同,这些变异是否与艾滋病痴呆综合征的发病机制有关,还有待进一步研究.
目的 研究艾滋病癡呆綜閤徵患者體內HIV-1 tat第一外顯子基因序列的特徵和變異情況,為艾滋病癡呆綜閤徵髮病機製的研究提供依據.方法 提取1例艾滋病癡呆綜閤徵病例尸檢標本的外週組織(淋巴結、脾髒)和中樞神經組織(腦膜、額葉灰質、額葉白質、顳葉、基底覈)共7箇部位的基因組DNA,巢式PCR擴增HIV-1 tat第一外顯子基因,與剋隆載體pGEM-T連接,經轉化、氨芐青黴素和藍白斑篩選齣暘性剋隆,每箇部位挑取5箇菌落測序,測序結果利用BioEdit、MEGA4軟件比對併生成繫統進化樹、計算基因距離和同義/非同義替換值(ds/dn),分析氨基痠位點的改變.結果 該病例感染的病毒是HIV-1 B亞型,分離自不同組織的HIV-1 tat第一外顯子基因序列存在差異;與標準序列相比,該病例的HIV-1tat第一外顯子基因編碼的氨基痠序列有16箇位點髮生瞭變異,併且中樞各部位部分氨基痠位點的變異與外週不同,特彆是中樞基底覈5箇序列及顳葉1箇序列Q54R的變異值得關註.結論 艾滋病癡呆綜閤徵患者體內,HIV-1 tat第一外顯子序列與標準序列存在差異,併且在外週和中樞不同部位中存在的變異不同,這些變異是否與艾滋病癡呆綜閤徵的髮病機製有關,還有待進一步研究.
목적 연구애자병치태종합정환자체내HIV-1 tat제일외현자기인서렬적특정화변이정황,위애자병치태종합정발병궤제적연구제공의거.방법 제취1례애자병치태종합정병례시검표본적외주조직(림파결、비장)화중추신경조직(뇌막、액협회질、액협백질、섭협、기저핵)공7개부위적기인조DNA,소식PCR확증HIV-1 tat제일외현자기인,여극륭재체pGEM-T련접,경전화、안변청매소화람백반사선출양성극륭,매개부위도취5개균락측서,측서결과이용BioEdit、MEGA4연건비대병생성계통진화수、계산기인거리화동의/비동의체환치(ds/dn),분석안기산위점적개변.결과 해병례감염적병독시HIV-1 B아형,분리자불동조직적HIV-1 tat제일외현자기인서렬존재차이;여표준서렬상비,해병례적HIV-1tat제일외현자기인편마적안기산서렬유16개위점발생료변이,병차중추각부위부분안기산위점적변이여외주불동,특별시중추기저핵5개서렬급섭협1개서렬Q54R적변이치득관주.결론 애자병치태종합정환자체내,HIV-1 tat제일외현자서렬여표준서렬존재차이,병차재외주화중추불동부위중존재적변이불동,저사변이시부여애자병치태종합정적발병궤제유관,환유대진일보연구.
Objective To study the variation and characteristics of HIV-1 tat exon 1 gene from a patient with AIDS dementia complex( ADC), so as to research the pathogenesis of ADC. Methods The tat gene was amplified with nested PCR from genomic DNA which was extracted from lymph node, spleen and different brain tissues( meninges, grey matter from frontal cortex, white matter from frontal cortex, temporal cortex and basal ganglia) of a patient who died of ADC. PCR products were cloned into the pGEM-T vector,after transformation and selection by ampicillin and blue/white spotting. Five of positive clones were sequenced. HIV-1 tat sequences were processed with BioEdit and MEGA4. With the softwares, Neighbor-Joining tree, p-Distances, values of ds/dn, and analysis of amino acid motifs were all done. Results The samples were all identified as HIV-1 B and genetic variation exists in HIV-1 tat isolated from different tissue;Compared with HXB2, sixteen sites of the amino acid seque nce coded by the HIV-1 tat gene which was isolated from the patient changed. In addition, part of the changes were different between periphery and brain,especially, the five Q54R changes from basal ganglia and one Q54R change from temporal cortex are deserve to follow with interest. Conclusion Variations exist in the HIV-1 tat genes extracted from the ADC patient and the variations from peripheral and central nerve tissues were different, whether the variations concerned with the pathogenesis of ADC need more research.