中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2008年
8期
872-875
,共4页
陈建国%戴晓莉%蒋玉凤%LIU Ying-zhao%虞建人%苏兆亮%HUANG Xin-xiang%张驰宇%WANG Sheng-jun%邵启祥%SHAO Shi-he%许化溪
陳建國%戴曉莉%蔣玉鳳%LIU Ying-zhao%虞建人%囌兆亮%HUANG Xin-xiang%張馳宇%WANG Sheng-jun%邵啟祥%SHAO Shi-he%許化溪
진건국%대효리%장옥봉%LIU Ying-zhao%우건인%소조량%HUANG Xin-xiang%장치우%WANG Sheng-jun%소계상%SHAO Shi-he%허화계
假单胞菌,铜绿%抗药性,多种,细菌%整合子类
假單胞菌,銅綠%抗藥性,多種,細菌%整閤子類
가단포균,동록%항약성,다충,세균%정합자류
Pseudomonas aeruginosa%Drug-resistance,multiple,bacterial%Integrons
目的 了解分离于镇江地区的铜绿假单胞菌对13种常用抗生素的耐药率;明确Ⅰ类整合子基因盒结构及其在耐药基因播散中的作用.方法 采用K-B纸片法检测71株铜绿假单胞菌的耐药率;煮沸法提取71株铜绿假单胞菌基因组DNA;PCR扩增Ⅰ类整合子基因,并通过测序分析其所携带耐药基因盒.结果 71株铜绿假单胞菌对临床常用的13种抗生素的耐药率在18.3%～77.5%不等;Ⅰ类整合子检出率为38%,包括aadB、aac(6')-Ⅱ、PSE-Ⅰ、dfrA17和aadA5 5种基因盒,其中最常见者为dfrA17和aadA5.Ⅰ类整合子阳性菌株对哌拉西林、哌拉西林/他唑巴坦、头孢曲松、头孢吡肟、头孢他啶、庆大霉素、阿米卡星、妥布霉素、左氧氟沙星、环丙沙星等10种抗生素的耐药率明显高于整合子阴性菌株.结论 不同铜绿假单胞菌临床株对13种常用抗生素的耐药率各不相同,整合子阳性菌株耐药率明显高于整合子阴性菌株,提示Ⅰ类整合子是铜绿假单胞菌多重耐药的重要因素.
目的 瞭解分離于鎮江地區的銅綠假單胞菌對13種常用抗生素的耐藥率;明確Ⅰ類整閤子基因盒結構及其在耐藥基因播散中的作用.方法 採用K-B紙片法檢測71株銅綠假單胞菌的耐藥率;煮沸法提取71株銅綠假單胞菌基因組DNA;PCR擴增Ⅰ類整閤子基因,併通過測序分析其所攜帶耐藥基因盒.結果 71株銅綠假單胞菌對臨床常用的13種抗生素的耐藥率在18.3%～77.5%不等;Ⅰ類整閤子檢齣率為38%,包括aadB、aac(6')-Ⅱ、PSE-Ⅰ、dfrA17和aadA5 5種基因盒,其中最常見者為dfrA17和aadA5.Ⅰ類整閤子暘性菌株對哌拉西林、哌拉西林/他唑巴坦、頭孢麯鬆、頭孢吡肟、頭孢他啶、慶大黴素、阿米卡星、妥佈黴素、左氧氟沙星、環丙沙星等10種抗生素的耐藥率明顯高于整閤子陰性菌株.結論 不同銅綠假單胞菌臨床株對13種常用抗生素的耐藥率各不相同,整閤子暘性菌株耐藥率明顯高于整閤子陰性菌株,提示Ⅰ類整閤子是銅綠假單胞菌多重耐藥的重要因素.
목적 료해분리우진강지구적동록가단포균대13충상용항생소적내약솔;명학Ⅰ류정합자기인합결구급기재내약기인파산중적작용.방법 채용K-B지편법검측71주동록가단포균적내약솔;자비법제취71주동록가단포균기인조DNA;PCR확증Ⅰ류정합자기인,병통과측서분석기소휴대내약기인합.결과 71주동록가단포균대림상상용적13충항생소적내약솔재18.3%～77.5%불등;Ⅰ류정합자검출솔위38%,포괄aadB、aac(6')-Ⅱ、PSE-Ⅰ、dfrA17화aadA5 5충기인합,기중최상견자위dfrA17화aadA5.Ⅰ류정합자양성균주대고랍서림、고랍서림/타서파탄、두포곡송、두포필우、두포타정、경대매소、아미잡성、타포매소、좌양불사성、배병사성등10충항생소적내약솔명현고우정합자음성균주.결론 불동동록가단포균림상주대13충상용항생소적내약솔각불상동,정합자양성균주내약솔명현고우정합자음성균주,제시Ⅰ류정합자시동록가단포균다중내약적중요인소.
Objective To investigate the antimicrobial susceptibility of Pseudomonas aeruginosa (P. aeruginosa) isolated from Zhenjiang area to 13 routinely used antibiotics and identify the structure and dissemination of class Ⅰ integron. Methods K-B test was used to determine the resistant rate of 71 strains of P. aeruginosa. DNA template was extracted by boiling method, PCR method was utilized to detect class Ⅰintegron, and subsequently gene cassettes were analyzed by sequencing. Results The resistant rates to 13 routinely used antibiotics were quite different from 18. 3 to 77.5% among 71 strains of P. aeruginosa. The prevalence of class Ⅰ integron was 38%. These integrons include 5 gene cassettes ( aadB, aac (6) - Ⅱ , PSE-Ⅰ , dfrA17 and aadAS), in which dfrA17 and aadA5 gene cassette were frequently found. Comparing with the negative strains of integron, the positive strains of integron has obviously higher resistance to ten the antibiotics including piporacillin, piperacillin-tazobactam, ceftriaxone, cefepime, ceftazidime, gentamicin,amikacin, tobmmycin, levofloxacin, and ciprofloxacin. Conclusions The resistant rates of P. aeruginosa to 13 drugs were different, and the resistant rates of integron positive strains were obviously higher than integron negative strains, which indicates that integron may play an important role in multidrug reisistance of P. aeruginoosa.
