中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2009年
7期
745-748
,共4页
张海莹%刘占东%胡朝军%张洋%冯雪%包书萌%张拥波%王得新%李永哲
張海瑩%劉佔東%鬍朝軍%張洋%馮雪%包書萌%張擁波%王得新%李永哲
장해형%류점동%호조군%장양%풍설%포서맹%장옹파%왕득신%리영철
疲劳综合征,慢性%自细胞,单核%转化生长因子β1%逆转录聚合酶链反应
疲勞綜閤徵,慢性%自細胞,單覈%轉化生長因子β1%逆轉錄聚閤酶鏈反應
피로종합정,만성%자세포,단핵%전화생장인자β1%역전록취합매련반응
Fatigue syndrome,chronic%Leukocytes,mononuclear%Transforming growth factor
目的 探讨慢性疲劳综合征(CFS)患者外周血单个核细胞(PBMCs)中转化生长因子β1(TGF-β1)mRNA及雌二醇受体p野生型(ERβwt)mRNA表达及其与CFS的发病关系.方法 用实时荧光定量逆转录(FQ-RT)-PCR检测63例CFS患者、50例其他疾病患者(疾病对照组)、50名健康体检者(健康对照组)外周血PBMCs中TGF-β1 mRNA和ERβwt mRNA的表达水平和含量.以△Ct=Ct(待测基因)-Ct(内参基因)来比较基因表达水平的变化.结果 CFS患者TGF-β1 mRNA表达水平(△Ct=3.27±0.58)明显高于健康对照组(△Ct=4.37±1.00,t=7.02,P<0.01)和疾病对照组(△Ct=4.54±1.05,t=8.11,P<0.01).CFS患者ERβwt mRNA表达水平(△Ct=9.34±0.92)明显低于健康对照组(△Ct=7.10±0.48,t=15.47,P<0.01)与疾病对照组(△Ct=7.12±0.47,t=15.44,P<0.01);结论CFS患者TGF-β1 mRNA表达水平显著高于健康人和疾病对照组;CFS患者ERβwtmRNA表达水平显著低于健康人和疾病对照组,TGF-β1 mRNA和ERβwt mRNA的表达可能参与了CFS的发病过程.
目的 探討慢性疲勞綜閤徵(CFS)患者外週血單箇覈細胞(PBMCs)中轉化生長因子β1(TGF-β1)mRNA及雌二醇受體p野生型(ERβwt)mRNA錶達及其與CFS的髮病關繫.方法 用實時熒光定量逆轉錄(FQ-RT)-PCR檢測63例CFS患者、50例其他疾病患者(疾病對照組)、50名健康體檢者(健康對照組)外週血PBMCs中TGF-β1 mRNA和ERβwt mRNA的錶達水平和含量.以△Ct=Ct(待測基因)-Ct(內參基因)來比較基因錶達水平的變化.結果 CFS患者TGF-β1 mRNA錶達水平(△Ct=3.27±0.58)明顯高于健康對照組(△Ct=4.37±1.00,t=7.02,P<0.01)和疾病對照組(△Ct=4.54±1.05,t=8.11,P<0.01).CFS患者ERβwt mRNA錶達水平(△Ct=9.34±0.92)明顯低于健康對照組(△Ct=7.10±0.48,t=15.47,P<0.01)與疾病對照組(△Ct=7.12±0.47,t=15.44,P<0.01);結論CFS患者TGF-β1 mRNA錶達水平顯著高于健康人和疾病對照組;CFS患者ERβwtmRNA錶達水平顯著低于健康人和疾病對照組,TGF-β1 mRNA和ERβwt mRNA的錶達可能參與瞭CFS的髮病過程.
목적 탐토만성피로종합정(CFS)환자외주혈단개핵세포(PBMCs)중전화생장인자β1(TGF-β1)mRNA급자이순수체p야생형(ERβwt)mRNA표체급기여CFS적발병관계.방법 용실시형광정량역전록(FQ-RT)-PCR검측63례CFS환자、50례기타질병환자(질병대조조)、50명건강체검자(건강대조조)외주혈PBMCs중TGF-β1 mRNA화ERβwt mRNA적표체수평화함량.이△Ct=Ct(대측기인)-Ct(내삼기인)래비교기인표체수평적변화.결과 CFS환자TGF-β1 mRNA표체수평(△Ct=3.27±0.58)명현고우건강대조조(△Ct=4.37±1.00,t=7.02,P<0.01)화질병대조조(△Ct=4.54±1.05,t=8.11,P<0.01).CFS환자ERβwt mRNA표체수평(△Ct=9.34±0.92)명현저우건강대조조(△Ct=7.10±0.48,t=15.47,P<0.01)여질병대조조(△Ct=7.12±0.47,t=15.44,P<0.01);결론CFS환자TGF-β1 mRNA표체수평현저고우건강인화질병대조조;CFS환자ERβwtmRNA표체수평현저저우건강인화질병대조조,TGF-β1 mRNA화ERβwt mRNA적표체가능삼여료CFS적발병과정.
Objective To detect the expression of TGF-β1 mRNA and ERβwt mRNA in peripheral blood monoanclear cells (PBMCs) in patients with chronic fatigue syndrome (CFS), and their relationship with pathogenesis of CFS. Methods Fluorescence quantitative RT-PCR (FQ-RT-PCR) was used to examine TGF-β1 mRNA and ERβwt mRNA expression of peripheral blood monocytes in 63 cases with CFS,50 cases with other diseases, and 50 healthy controls. The gene expression levels were calculated with the formula △Ct=Ct(target gene) - Ct(internal control). Results The mean TGF-β1 mRNA expression of CFS patients (△Ct = 3.27 ± 0. 58) was higher than that of disease controls (△Ct = 4. 54 ± 1.05, t = 8. 11, P <0.01) and that of healthy controls (△Ct = 4. 37 ± 1.00, t = 7. 02, P < 0. 01). The mean ERβwt mRNA expression of CFS patients (△Ct =9. 34 ±0. 92) was lower than that of disease controls(△Ct =7.12±0. 47, t = 15.44 ,P < 0. 01) and that of healthy controls(△Ct = 7. 10 ±0. 48, t = 15.47, P < 0. 01). Conclusions The TGF-β1 mRNA and ER βwt mRNA expression levels of PBMCs are siguificantly elevated in patients with CFS. It may be implicated in the pathogenesis of CSF.
