中国科学B辑(英文版)
中國科學B輯(英文版)
중국과학B집(영문판)
SCIENCE IN CHINA(SCIENTIA SINICA)
2002年
3期
225-231
,共7页
储结根%刘晓航%袁直%何炳林
儲結根%劉曉航%袁直%何炳林
저결근%류효항%원직%하병림
uremic middle molecules%reversed-phase high performance liquid chromatography%matrix assisted laser desorption/ionization%time-of-flight mass spectrometry
Isolation and comparison of uremic sera and urine and normal sera and urine were performed by gel permeation chromatography, anion exchange chromatography and reversed-phase high performance liquid chromatography. Two uremic middle molecular fractions (A and B) were obtained from uremic sera and urine and normal urine by gel permeation chromatography, but not from normal sera. The anion exchange chromatographic results of fraction A from different origins demonstrate that subfraction A-3 could be excreted in urine by healthy subject, but accumulated in uremic serum for renal failure of patient with uremia. After desalinization subfraction A-3 was analyzed by MALDI-TOF-MS. The results show that subfraction A-3 consists of six compounds with molecular weight 839, 873, 1007.94, 1106, 1680 and 2015 respectively. Finally, by reversed-phase high performance liquid chromatography, subfraction A-3 was further resolved into six independent fractions. Thus, the isolation and purification of six middle molecular compounds in subfraction A-3 came true by our method.
