CAJ | 학술논문

携带普通双元载体的根癌土壤杆菌（Agrobacterium tumefaciens (Smith et Townsend) Conn) EHA105菌株对粳稻(Oryza sativa L.ssp.　japonica)中花8号不敏感，转化效率较低。利用基因枪将根癌土壤杆菌细胞直接轰击到水稻愈伤组织中可以显著提高其转化效率。Southern检测证明转基因植物含有单拷贝的外源基因插入片段。转基因植物后代的GUS基因表达呈3∶1分离。
휴대보통쌍원재체적근암토양간균（Agrobacterium tumefaciens (Smith et Townsend) Conn) EHA105균주대갱도(Oryza sativa L.ssp.　japonica)중화8호불민감，전화효솔교저。이용기인창장근암토양간균세포직접굉격도수도유상조직중가이현저제고기전화효솔。Southern검측증명전기인식물함유단고패적외원기인삽입편단。전기인식물후대적GUS기인표체정3∶1분리。
Oryza sativa L. ssp. japonica cv. Zhonghua 8, which is recalcitrant to infection of Agrobacterium tumefaciens (Smith et Townsend) Conn strain EHA105 with ordinary binary vector pCambia 1301, was transformed through Agrobacterium-mediated transformation with help of bombardment. The transformation efficiency can be raised greatly. Single copy of gene insertion in the genome of transgenic rice plants was proved by Southern analysis and the expression of GUS gene was observed. GUS gene and hygromycin_resistant gene show 3∶1 segregation in progenies of the transgenic rice plants.