CAJ | 학술논문

应用DNA电泳分析、HO33342和TUNEL染色法、以及部分地使用透射电镜技术,检测了不同浓度的溶血磷脂酸(1ysophosphatidic acid,LPA)对离体培养的小鼠大脑皮层神经元存活情况的影响.结果显示,低浓度的LPA(0.1～30μmol/L)对去血清培养所致的皮层神经元凋亡有浓度依赖性的保护作用,而较高浓度的LPA(＞50 μmol/L)不仅不表现这种保护作用,而且可引致培养在含血清的完全培养基中的皮层神经元出现凋亡.以上结果表明,适当浓度的LPA对凋亡的皮层神经元起着保护因子或抗凋亡因子的作用,而较高浓度的LPA则起着促凋亡因子的作用.
응용DNA전영분석、HO33342화TUNEL염색법、이급부분지사용투사전경기술,검측료불동농도적용혈린지산(1ysophosphatidic acid,LPA)대리체배양적소서대뇌피층신경원존활정황적영향.결과현시,저농도적LPA(0.1～30μmol/L)대거혈청배양소치적피층신경원조망유농도의뢰성적보호작용,이교고농도적LPA(＞50 μmol/L)불부불표현저충보호작용,이차가인치배양재함혈청적완전배양기중적피층신경원출현조망.이상결과표명,괄당농도적LPA대조망적피층신경원기착보호인자혹항조망인자적작용,이교고농도적LPA칙기착촉조망인자적작용.
The effect of lysophosphatidic acid (LPA), with a wide range of its different concentrations, upon cultured mouse cortical neurons was assessed by electrophoresis of DNA fragments, HO33342 and TUNEL stainings, and also by ultrastructural examination at times. The results showed that administration of LPA at lower concentrations (0.1～30 μmol/L) dose-dependently protected cortical neurons from apoptosis that was induced by deprivation of serum from the cultural medium, while 50 μtmol/L or higher concentrations of LPA failed to show this effect; and moreover, the concentrations higher than 50 μmol/L induced apoptosis in neurons cultured in serumcontaining complete medium. These results suggest that a moderate concentration of LPA may play as a survival factor in apoptotic cortical neurons, while an excessive level of LPA induces apoptosis in neurons cultured in complete medium.