车前子调整脂代谢及其抗氧化作用
차전자조정지대사급기항양화작용
Regulation of plantain seed on lipid metabolism and its antioxidation
  • 万方数据
    中国临床康复 중국림상강복
    CHINESE JOURNAL OF CLINICAL REHABILITATION
    2005年 31期 248-250 ,共3页

    王素敏%黎燕峰%代洪燕%裴庭梅%王永利

    왕소민%려연봉%대홍연%배정매%왕영리

    车前子%高脂血症%抗氧化 車前子%高脂血癥%抗氧化
    차전자%고지혈증%항양화
    背景:抗氧化效能的营养素对脂质过氧化反应的抑制作用和对心血管疾病预防作用正日益受到人们的关注.目的:探讨具有清除氧自由基、抗氧化作用的车前子对大鼠脂代谢及抗氧化作用的影响.设计:随机对照实验.单位:河北省新药药理毒理实验室.对象:实验于2004-01/12在河北省新药药理毒理研究实验室完成.40只SD大鼠由河北省实验动物中心提供,健康一级,体质量(210±22)g,雌雄兼用.随机分为5组,空白对照组、阳性对照组、低剂量实验组、中剂量实验组和高剂量实验组,每组8只.方法:空白对照组每天以基础饲料喂养,符合AoAc动物营养标准,自由饮水;阳性对照组每天以高脂饲料喂养,自由饮水;车前子低、中、高剂量组在高脂饲料的基础上分别加入2.5 g/kg,5 g/kg和15 g/kg的车前子,自由饮水.每周称重,记进食量,每隔两周采空腹血测定血清总胆固醇,12周后实验结束,麻醉下腹主动脉采血检测血清血脂水平和超氧化物歧化酶、过氧化氢酶的活性.取血后,立即取出心、肝进行处理,检测其心肌组织中超氧化物歧化酶的活性和脂质过氧化物的含量及肝组织中过氧化氢酶和谷光甘肽过氧化物酶的活性.主要观察指标:大鼠血脂水平和超氧化物歧化酶及过氧化氢酶的活性.结果:纳入40只大鼠均进入结果分析.[1]血清总胆固醇:车前子高剂量组显著低于阳性对照组[(1.40±0.13,1.83±0.13)mmol/L,(P＜0.05)].[2]血清超氧化物歧化酶活性:阳性对照组明显低于空白对照组[(174.29±10.33,193.19±.7813)NU/mg,(P＜0.05)].[3]心肌组织中脂质过氧化物含量:阳性对照组显著高于空白对照组[(3.64±0.26,2.91±0.50)mmol/mg,(P＜0.05)];车前子中剂量组显著低于阳性对照组[(3.13±0.26,3.64±0.26)mmoL/mg,(P＜0.05)].[4]肝组织中过氧化氢酶活性:阳性对照组明显低于正常对照组[(34.64±3.26,44.72±2.67)NU/mg,(P＜0.05)];车前子中剂量组明显高于阳性对照组[(44.84±3.79,34.96±3.64)Nu/mg,(P＜0.05)].结论:车前子可降低大鼠血清总胆固醇、三酰甘油和脂质过氧化物水平并提高超氧化物歧化酶活性,在浓度为15g/kg时车前子清除氧自由基、抗氧化的作用最明显,可减轻脂质代谢紊乱.
    배경:항양화효능적영양소대지질과양화반응적억제작용화대심혈관질병예방작용정일익수도인문적관주.목적:탐토구유청제양자유기、항양화작용적차전자대대서지대사급항양화작용적영향.설계:수궤대조실험.단위:하북성신약약리독리실험실.대상:실험우2004-01/12재하북성신약약리독리연구실험실완성.40지SD대서유하북성실험동물중심제공,건강일급,체질량(210±22)g,자웅겸용.수궤분위5조,공백대조조、양성대조조、저제량실험조、중제량실험조화고제량실험조,매조8지.방법:공백대조조매천이기출사료위양,부합AoAc동물영양표준,자유음수;양성대조조매천이고지사료위양,자유음수;차전자저、중、고제량조재고지사료적기출상분별가입2.5 g/kg,5 g/kg화15 g/kg적차전자,자유음수.매주칭중,기진식량,매격량주채공복혈측정혈청총담고순,12주후실험결속,마취하복주동맥채혈검측혈청혈지수평화초양화물기화매、과양화경매적활성.취혈후,립즉취출심、간진행처리,검측기심기조직중초양화물기화매적활성화지질과양화물적함량급간조직중과양화경매화곡광감태과양화물매적활성.주요관찰지표:대서혈지수평화초양화물기화매급과양화경매적활성.결과:납입40지대서균진입결과분석.[1]혈청총담고순:차전자고제량조현저저우양성대조조[(1.40±0.13,1.83±0.13)mmol/L,(P＜0.05)].[2]혈청초양화물기화매활성:양성대조조명현저우공백대조조[(174.29±10.33,193.19±.7813)NU/mg,(P＜0.05)].[3]심기조직중지질과양화물함량:양성대조조현저고우공백대조조[(3.64±0.26,2.91±0.50)mmol/mg,(P＜0.05)];차전자중제량조현저저우양성대조조[(3.13±0.26,3.64±0.26)mmoL/mg,(P＜0.05)].[4]간조직중과양화경매활성:양성대조조명현저우정상대조조[(34.64±3.26,44.72±2.67)NU/mg,(P＜0.05)];차전자중제량조명현고우양성대조조[(44.84±3.79,34.96±3.64)Nu/mg,(P＜0.05)].결론:차전자가강저대서혈청총담고순、삼선감유화지질과양화물수평병제고초양화물기화매활성,재농도위15g/kg시차전자청제양자유기、항양화적작용최명현,가감경지질대사문란.
    BACKGROUND: Great concerns haven been given increasingly on inhibition of nutrient with antioxidation efficacy on lipid peroxidation and its effect on prevention of cardiac vascular disease.OBJECTIVE: To probe into the effects of plantain seed that acts on eliminating oxygenic free radical and antioxidation on lipid metabolism and antioxidation in rats.DESIGN: Randomized control experiment was designed.SETTING: Experimental Room of Pharmacology and Toxicology of New Drug in Hebei Province.PARTICIPANTS: The experiment was performed in Experimental Room of Pharmacology and Toxicology of New Drug in Hebe Province from January to December 2004, in which, 40 SD rats were employed, provided from Hebei Experimental Animal Center, of healthy grade I, mass weighted (210±22) g and of either sex. They were randomized into 5groups, named, blank control, positive control, low dosage experiment group, moderate dosage experiment group and high dosage experiment group, 8 rats in each one.METHODS: In blank control, the rats were bred everyday with basic forage that was tallied with AoAc animal nutrient criteria and they were free of drinking. In positive control, the rats were bred with high-lipid forage and free of drinking. In the groups of low, moderate and high dosages of plantain seed, the rats were bred with 2.5 g/kg, 5 g/kg and 15 g/kg plantain seed successively besides high-lipid forage and they were free of drinking. The weight was measured and the food intake was recorded every week. Fasting blood was collected to check total cholesterol in serum once every two weeks. The experiment was end in 12 weeks. Under anesthesia,the blood was collected from hypogastric aorta to check the level of serum blood lipid and the activities of superoxide dismutase (SOD) and catalase.After blood collection, the heart and liver were extracted immediately for management to measure SOD activity and content of lipid peroxide (LPO)in myocardial tissue and the activities of catalase and glutathione peroxidase in liver tissue.MAIN OUTCOME MEASURES: Level of blood lipid and activities of SOD and catalase in rats.RESULTS: Forty rats were employed and all entered result analysis. [1]Serum total cholesterol: It was lower significantly in high dosage group compared with positive control [(1.40±0.13, 1.83±0.13) mmol/L, P ＜ 0.05].[2] Serum SOD activity: It was lower remarkably in positive control compared with blank control [(174.29±10.33, 193.19±.7813) NU/mg, P ＜ 0.05].[3] LPO content in serum: It was higher significantly in positive control compared with blank control [(3.64±0.26, 2.91±0.50) mmol/mg, P ＜ 0.05]and it was lower significantly in moderate dosage group compared with positive control (3.13±0.26, 3.64±0.26, P ＜ 0.05). [4] Activity of catalase in liver tissue: It was lower remarkably in positive control compared with normal control (34.64±3.26, 44.72±2.67, P ＜ 0.05) and it was higher remarkably in moderate dosage group compared with positive control (44.84±3.79,34.96±3.64, P ＜ 0.05).CONCLUSION: Plantain seed reduces the levels of total cholesterol,triglycerin (TG) and LPO in serum and increases SOD activity. At the concentration of 15 g/kg, plantain seed acts most remarkably on eliminating oxygenic free radical and antioxidation and alleviates lipid metabolic disturbance.
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