CAJ | 학술논문

目的建立反相高效液相色谱法测定大鼠血浆中的葛根素,用于葛根素微乳在大鼠体内的生物利用度研究.方法取血浆样品,用乙腈沉淀蛋白,Phenomenex C18柱(250 mm×4.6 mm,5 μm)分离,4-羟基苯甲醛为内标,乙腈-0.1%醋酸溶液(23:77)为流动相,流速0.8 mL·min-1,紫外检测波长250 nm,柱温30 ℃,进样量 20 μL.结果葛根素的线性范围为0.10～10.00 μg·mL-1(r=0.999 6),定量下限为0.10 μg·mL-1,提取回收率＞79%,方法回收率＞92%,日内、日间精密度RSD＜6%.结论本方法灵敏度好、准确性高,可用于大鼠血浆中葛根素浓度的测定以及葛根素微乳在大鼠体内的生物利用度研究.
목적 건립반상고효액상색보법측정대서혈장중적갈근소,용우갈근소미유재대서체내적생물이용도연구.방법 취혈장양품,용을정침정단백,Phenomenex C18주(250 mm×4.6 mm,5 μm)분리,4-간기분갑철위내표,을정-0.1%작산용액(23:77)위류동상,류속0.8 mL·min-1,자외검측파장250 nm,주온30 ℃,진양량 20 μL.결과 갈근소적선성범위위0.10～10.00 μg·mL-1(r=0.999 6),정량하한위0.10 μg·mL-1,제취회수솔＞79%,방법회수솔＞92%,일내、일간정밀도RSD＜6%.결론 본방법령민도호、준학성고,가용우대서혈장중갈근소농도적측정이급갈근소미유재대서체내적생물이용도연구.
Objective To establish an RP-HPLC method for the determination of puerarin in rat plasma to be applied in the bioavailability study of puerarin microemulsion in rats.Methods Plasma samples were treated with acetonitrile to deposit proteins,and then were analyzed by HPLC on a Phenomenex C18 column(250 mm×4.6 mm,5 μm) using acetonitrile-0.1% acetic acid solution(23∶77)mixture solvent as the mobile phase at 0.8 mL·min-1.The column effluent was monitored by UV detector at 250 nm and 4-hydroxy-benzaldehyde was used as the internal standard.The column temperature was 30 ℃,and the injection volume was 20 μL.Results The calibration curve of puerarin in rat plasma at 0.10～10.00 μg·mL-1 was excellently linear(r=0.999 6),with the lower limit of quantification of 0.10 μg·mL-1.The extraction recoveries were larger than 79%,the method recoveries larger than 92%,and the intra- and inter-day RSD smaller than 6%.Conclusion The validated method is sensitive,accurate and suitable for the determination of puerarin in rat plasma and bioavailability study of puerarin microemulsion in rats.