CAJ | 학술논문

利用生物信息学方法对来自2个不同cDNA文库的2万余条杨树EST序列进行了候选SNPs标记.研究结果表明,在获得的94个碱基替代型候选SNPs中,A/G替换类型最多,占32.98%,C/T替换型占13.83%,其它碱基置换型SNPs占53.20%.同时,对其中20个候选SNPs位点的重新测序结果表明,有13个(占65%)候选sNPs位点得到证实,其中,11个位点为碱基替代型SNPs,另外2个为杂合型位点.
이용생물신식학방법대래자2개불동cDNA문고적2만여조양수EST서렬진행료후선SNPs표기.연구결과표명,재획득적94개감기체대형후선SNPs중,A/G체환류형최다,점32.98%,C/T체환형점13.83%,기타감기치환형SNPs점53.20%.동시,대기중20개후선SNPs위점적중신측서결과표명,유13개(점65%)후선sNPs위점득도증실,기중,11개위점위감기체대형SNPs,령외2개위잡합형위점.
More than 20 000 EST sequences of poplar were clustered and stringent filters were used to identify reliable candidate SNPs. Sequences analysis indicated that totally 94 candidate SNPs were found. The main types of substitutions among candidate SNPs were A/G, C/T and other types, which accounted for 32. 98%, 13. 83%, and 53. 20%, respectively. Resequencing with 6 samples for 20 candidate SNPs showed that 13 candidate SNPs were verified, accounting for 65 %. And among them, 11 SNPs were substitution type and the other 2 SNPs were heterozygous type. The detailed information about these candidate SNPs would provide a basis for further marker identification and application.