CAJ | 학술논문

为揭示盾叶薯蓣类原球茎发育的细胞学基础和薯蓣皂苷在各离体培养繁殖体中的分布,应用石蜡切片技术、扫描电镜和透射电镜制片技术及组织化学方法进行了研究.制片观察结果显示:盾叶薯蓣类原球茎是由多层鳞片叶包裹的致密小球体,从外至内由周皮、基本组织和分散在基本组织中的维管束组成,初生增厚分生组织和原形成层是其增大的主要原因之一;组织化学结果显示:愈伤组织和类原球茎中均有薯蓣皂苷的分布,而表皮栓质化的愈伤组织和生根类原球茎中薯蓣皂苷的含量相对较多.
위게시순협서여류원구경발육적세포학기출화서여조감재각리체배양번식체중적분포,응용석사절편기술、소묘전경화투사전경제편기술급조직화학방법진행료연구.제편관찰결과현시:순협서여류원구경시유다층린편협포과적치밀소구체,종외지내유주피、기본조직화분산재기본조직중적유관속조성,초생증후분생조직화원형성층시기증대적주요원인지일;조직화학결과현시:유상조직화류원구경중균유서여조감적분포,이표피전질화적유상조직화생근류원구경중서여조감적함량상대교다.
By using the techniques of light microscope, scanning electron microscope, transmission electron microscope and histochemical localization of dioscin, the cytoarchitecture feature and dioscin distribution of the protocorm-like body(PLB) of Dioscorea zingiberensis C . H. Wright were studied. The results showed the PLB, a compact corm wrapped by scale leaves, consisted of periderm and vascular bundle scattered among the ground tissue, PLB enlargement largely due to the cell division of the primary thicking meristem(PTM) and procambium. Furthermore, callus with suberized epidermis and rooted PLB stained more intensely than the tenders, which indicated more diosgenin located within the old tissues in vitro.