CAJ | 학술논문

实验以EDTA和不同金属离子为效应物,果胶为反应底物,研究不同浓度的EDTA,Na~+,K~+,Ca~(2+),Cu~(2+),Zn~(2+),Mn~(2+),Fe~(2+),Fe~(3+),Ag+和Ba~(2+)对多聚半乳糖醛酸酶(PG)催化活性的影响.结果表明,一定浓度的Cu~(2+),Zn~(2+),Mn~(2+),Fe~(2+)和Fe~(3+)对PG有激活作用,对PG产生最大激活作用的浓度分别为0.3mmol/L,0.4mmol/L,0.3mmol/L,0.3mmol/L和0.6mmol/L,EDTA,Ca~(2+),Ag+和Ba~(2+)对PG有抑制作用,Na+和K+对PG活性没有影响.动力学研究表明,0.3mmol/L Na~+和K+处理后,PG的K_m和V_(max)基本没有发生变化,2mmol/L EDTA和0.3mmol/L Ca~(2+)处理后,PG的K_m和V_(max)均下降,0.3mmol/L Cu~(2+) ,Zn~(2+),Mn~(2+),Fe~(2+),Fe~(3+)处理后,PG的K_m和V_(max)都上升,0.3mmol/L Ag+和Ba~(2+)处理后,PG的K_m上升,V_(max)下降.
실험이EDTA화불동금속리자위효응물,과효위반응저물,연구불동농도적EDTA,Na~+,K~+,Ca~(2+),Cu~(2+),Zn~(2+),Mn~(2+),Fe~(2+),Fe~(3+),Ag+화Ba~(2+)대다취반유당철산매(PG)최화활성적영향.결과표명,일정농도적Cu~(2+),Zn~(2+),Mn~(2+),Fe~(2+)화Fe~(3+)대PG유격활작용,대PG산생최대격활작용적농도분별위0.3mmol/L,0.4mmol/L,0.3mmol/L,0.3mmol/L화0.6mmol/L,EDTA,Ca~(2+),Ag+화Ba~(2+)대PG유억제작용,Na+화K+대PG활성몰유영향.동역학연구표명,0.3mmol/L Na~+화K+처리후,PG적K_m화V_(max)기본몰유발생변화,2mmol/L EDTA화0.3mmol/L Ca~(2+)처리후,PG적K_m화V_(max)균하강,0.3mmol/L Cu~(2+) ,Zn~(2+),Mn~(2+),Fe~(2+),Fe~(3+)처리후,PG적K_m화V_(max)도상승,0.3mmol/L Ag+화Ba~(2+)처리후,PG적K_m상승,V_(max)하강.
In this experiment,EDTA and different metal ions were used as effectors,and pectin as substrate in order to study the the effects of EDTA and different metal ions on enzyme specific activity of polygalacturonase(PG).The metal ions included Na~+,K~+,Ca~(2+),Cu~(2+),Zn~(2+),Mn~(2+),Fe~(2+),Fe~(3+),Ag~+ and Ba~(2+). The result showed that the enzyme specific activity of PG increased after the treatment with certain concentrations of Cu~(2+),Zn~(2+),Mn~(2+),Fe~(2+) and Fe~(3+).The concentration which caused the max activation of PG of Cu~(2+),Zn~(2+),Mn~(2+),Fe~(2+) and Fe~(3+) were 0.3 mmol/L,0.4 mmol/L,0.3 mmol/L,0.3 mmol/L and 0.6 mmol/L,respectively.EDTA,Ca~(2+),Ag~+ and Ba~(2+) restrained the activity of PG. Na+ and K+ but had no effect on the activity of PG. Kinetics analysis shown that the treatment of Na~+ and K~+ with the concentration of 0.3 mmol/L had no effect on K_m and V_(max) of PG.The treatment with 2 mmol/L EDTA and 0.3 mmol/L Ca~(2+) decreased the K_m and V_(max) of PG.And after the treatment of Cu~(2+),Zn~(2+),Mn~(2+),Fe~(2+) and Fe~(3+) with the concentration of 0.3mmol/L,the K_m and V_(max) of PG increased. However,the treatment of 0.3 mmol/L Ag~+ and Ba~(2+) increased the K_m of PG and decreased V_(max)of PG.