天然产物研究与开发
天然產物研究與開髮
천연산물연구여개발
NATURAL PRODUCT RESEARCH AND DEVELOPMENT
2009年
6期
984-987
,共4页
李进伟%范柳萍%丁霄霖%张连富
李進偉%範柳萍%丁霄霖%張連富
리진위%범류평%정소림%장련부
金丝小枣%多糖%分离纯化%组成
金絲小棘%多糖%分離純化%組成
금사소조%다당%분리순화%조성
Zizyphus jujuba cv.Jinsixiaozao%polysaccharide%isolation and purification%composition
经DEAE-SepharoseCL-6B、SepharoseCL-6B和Sephadex G-200柱层析,得到一个均一的金丝小枣多糖组分(ZP3c).ZP3c的中性单糖是由鼠李糖、阿拉伯糖和半乳糖组成,其摩尔比为1:2.13:19.3.经红外光谱测定,ZP3c的酯化度为41.85%.
經DEAE-SepharoseCL-6B、SepharoseCL-6B和Sephadex G-200柱層析,得到一箇均一的金絲小棘多糖組分(ZP3c).ZP3c的中性單糖是由鼠李糖、阿拉伯糖和半乳糖組成,其摩爾比為1:2.13:19.3.經紅外光譜測定,ZP3c的酯化度為41.85%.
경DEAE-SepharoseCL-6B、SepharoseCL-6B화Sephadex G-200주층석,득도일개균일적금사소조다당조분(ZP3c).ZP3c적중성단당시유서리당、아랍백당화반유당조성,기마이비위1:2.13:19.3.경홍외광보측정,ZP3c적지화도위41.85%.
a water-soluble polysaccharide(ZP3c)was purified by DEAE-SephatoseCL-6B,Sepharose CL-6B and Sephadex G-200 column chromatography.The purity of ZP3c was verified by high-performance liquid chromatography (HPLC),specific rotatory power teat and agaroae gel eleetrophoresis.The average molecular weight WaS estimated to be 1.6×105 Da.GC analysis result showed that ZP3c was composed mainly of rhamnose,arabinose and galactose in a molar ratio of 1:2.13:19.3.The degree of esterifieafion(DE)value of ZP3c was 41.85% by Fourier transform infrared spectrophotometer.
