中国癌症杂志
中國癌癥雜誌
중국암증잡지
CHINA ONCOLOGY
2010年
1期
12-16
,共5页
罗小军%孔宪炳%阎雄%周江山
囉小軍%孔憲炳%閻雄%週江山
라소군%공헌병%염웅%주강산
Sonic Hedgehog信号通路%肝细胞癌%表达
Sonic Hedgehog信號通路%肝細胞癌%錶達
Sonic Hedgehog신호통로%간세포암%표체
Sonic Hedgehog signaling pathway%hepatocellular carcinoma%expression
背景与目的:有研究表明Sonic Hedgehog(SHH)信号通路参与多种肿瘤的发生和发展,本研究通过检测SHH信号通路中蛋白Shh、Gli2在肝细胞癌(hepatocellular carcinoma,HCC)中的表达情况,探讨其与HCC各临床病理特征的关系和意义.方法:采用免疫组织化学法检测30例肝癌组织及10例正常肝组织中蛋白Shh、G1i2的表达;RT-PCR法检测10例HCC组织及相应癌旁组织中和肝痛细胞系HepG2、Huh7中Shh和Gli2mRNA的表达.结果:免疫组织化学法检测结果显示Shh、Gli2在HCC组织中阳性率分别为63.3%(19/30)和66.7%(20/30);Gli2的表达与HCC病理分级和肝门静脉侵犯相关(P=0.017,P=0.024).Shh、Gli2在正常肝组织中无表达.RT-PCR检测结果显示HCC组织和HepG2、Huh7细胞系中都存在Shh、Gli2 mRNA的表达.HCC组织中Shh、Gli2基因表达高于癌旁组织(P<0.05);Shh、Gli2 mRNA在Huh7中的表达强度高于HepG2,但两者间差异无统计学意义(P>0.05).结论:Shh和Gli2在HCC细胞系和组织中的高表达,可能参与了肝癌的发生和发展,为肝癌的防治研究提供了新的实验依据.
揹景與目的:有研究錶明Sonic Hedgehog(SHH)信號通路參與多種腫瘤的髮生和髮展,本研究通過檢測SHH信號通路中蛋白Shh、Gli2在肝細胞癌(hepatocellular carcinoma,HCC)中的錶達情況,探討其與HCC各臨床病理特徵的關繫和意義.方法:採用免疫組織化學法檢測30例肝癌組織及10例正常肝組織中蛋白Shh、G1i2的錶達;RT-PCR法檢測10例HCC組織及相應癌徬組織中和肝痛細胞繫HepG2、Huh7中Shh和Gli2mRNA的錶達.結果:免疫組織化學法檢測結果顯示Shh、Gli2在HCC組織中暘性率分彆為63.3%(19/30)和66.7%(20/30);Gli2的錶達與HCC病理分級和肝門靜脈侵犯相關(P=0.017,P=0.024).Shh、Gli2在正常肝組織中無錶達.RT-PCR檢測結果顯示HCC組織和HepG2、Huh7細胞繫中都存在Shh、Gli2 mRNA的錶達.HCC組織中Shh、Gli2基因錶達高于癌徬組織(P<0.05);Shh、Gli2 mRNA在Huh7中的錶達彊度高于HepG2,但兩者間差異無統計學意義(P>0.05).結論:Shh和Gli2在HCC細胞繫和組織中的高錶達,可能參與瞭肝癌的髮生和髮展,為肝癌的防治研究提供瞭新的實驗依據.
배경여목적:유연구표명Sonic Hedgehog(SHH)신호통로삼여다충종류적발생화발전,본연구통과검측SHH신호통로중단백Shh、Gli2재간세포암(hepatocellular carcinoma,HCC)중적표체정황,탐토기여HCC각림상병리특정적관계화의의.방법:채용면역조직화학법검측30례간암조직급10례정상간조직중단백Shh、G1i2적표체;RT-PCR법검측10례HCC조직급상응암방조직중화간통세포계HepG2、Huh7중Shh화Gli2mRNA적표체.결과:면역조직화학법검측결과현시Shh、Gli2재HCC조직중양성솔분별위63.3%(19/30)화66.7%(20/30);Gli2적표체여HCC병리분급화간문정맥침범상관(P=0.017,P=0.024).Shh、Gli2재정상간조직중무표체.RT-PCR검측결과현시HCC조직화HepG2、Huh7세포계중도존재Shh、Gli2 mRNA적표체.HCC조직중Shh、Gli2기인표체고우암방조직(P<0.05);Shh、Gli2 mRNA재Huh7중적표체강도고우HepG2,단량자간차이무통계학의의(P>0.05).결론:Shh화Gli2재HCC세포계화조직중적고표체,가능삼여료간암적발생화발전,위간암적방치연구제공료신적실험의거.
Background and purpose: It was reported that Sonic Hedgehog (SHH) signaling pathway was involved in carcinogenesis and progression of various tumor types. This study was designed to observe the expression of Shh and Gli2 in SHH signaling pathway in hepatocellular carcinoma (HCC) and to study the relationship between the expression of Shh and Gli2 and different clinicopathlogical characteristics, and further to explore the clinical significance. Methods: We studied 30 HCC tissues and 10 normal liver tissue slices using immunochemistry for the expression of Shh and Gli2, 10 fresh HCC tissues, adjacent-tumor tissues and 2 HCC cell lines HepG2, Huh7 by using RT-PCR for the mRNA expression of Shh and Gli2. Results: Immunochemistry showed in 30 HCC tissue slices,the positive ratios of the components of SHH signaling pathway Shh, Gli2 were 63.3% (19/30) and 66.7% (20/30), respectively. Expression of Gli2 was significantly correlated with the pathological grade and the tumor invasion in hepatic portal vein (P=0.017 and P=0.024). Shh and Gli2 did not express in normal liver tissue. RT-PCR showed Shh and Gli2 were detected as positive in both HCC tissues and lines. The expressions of Shh mRNA and Gli2 mRNA were higher in HCC than in adjacent-tumor tissues (P<0.05). The expressions of Shh mRNA and Gli2 mRNA were higher in Huh7 than in HepG2 cell lines, but no significant difference was found between them (P>0.05). Conclusion: The expressions of Shh and Gli2 were elevated in HCC, which indicated that SHH signaling pathway may be involved in human HCC carcinogenesis. The study may be useful for treatment of HCC.
