CAJ | 학술논문

大白菜是中国北方的重要蔬菜之一，根肿病是危害大白菜生产世界性病害，利用花药培养可以大大加速抗根肿病大白菜杂交育种工作的进程。对33份抗根肿病大白菜品种（品系）进行花药培养，有24个品种诱导出胚，品种诱导率为72．7％，以东方皇冠×C11的诱导率最高，为1．86胚／蕾。对大部分基因型来说，在培养基中添加0．4g／L谷氨酰胺的诱导效果最好。研究还发现生长在温室中的供体植株更容易诱导出胚状体。变绿的子叶型胚转到B5＋0．2mg／LBA＋0．1mg／LNAA＋0．1％活性炭的胚分化培养基上可诱导生芽。
대백채시중국북방적중요소채지일，근종병시위해대백채생산세계성병해，이용화약배양가이대대가속항근종병대백채잡교육충공작적진정。대33빈항근종병대백채품충（품계）진행화약배양，유24개품충유도출배，품충유도솔위72．7％，이동방황관×C11적유도솔최고，위1．86배／뢰。대대부분기인형래설，재배양기중첨가0．4g／L곡안선알적유도효과최호。연구환발현생장재온실중적공체식주경용역유도출배상체。변록적자협형배전도B5＋0．2mg／LBA＋0．1mg／LNAA＋0．1％활성탄적배분화배양기상가유도생아。
Chinese cabbage is one of the important vegetables in northern China. Clubroot disease seriously influenced the production of Chinese cabbage in the world. The use of anther culture can greatly accelerate breeding process of Chinese cabbage resistant to clubroot. Thirty three Chinese cabbage varieties （lines） resistant to clubroot were used to anther culture in this study. The results indicated that the embryo induction rate of varieties were 72.7%, and the highest rate was 1. 86 embryos / bud for Oriental Crown × C11 lines. The medium plus 0.4 g/L glutamine was best for induction of most genotypes. The results also showed that donor plants growing in the greenhouse were easier to induce embryoids. Green cotyledon-type embryos were transferred to solid medium with B5 ＋ 0.2 mg/L BA ＋ 0.1 mg/L NAA ＋ 0.1% activated carbon to induce adventitious buds.