CAJ | 학술논문

目的研究阿魏酸钠对慢性低氧大鼠肺血管形态、p53及PCNA蛋白表达及细胞增殖与凋亡的影响.方法 30只雄性SD大鼠采用随机单位组设计方法分为对照组、低氧组和联合治疗组,每组10只.采用常压间断低氧8h/d,连续21d的方法复制慢性低氧性肺动脉高压模型.对照组吸入空气(其他条件与低氧组相同).联合治疗组大鼠腹腔注射15 ms/(kg·d)的阿魏酸钠.观察阿魏酸钠对慢性低氧大鼠肺血管形态学指标、p53及增殖细胞核抗原PCNA基因蛋白表达、细胞的增殖与凋亡的影响.结果低氧21 d后,低氧组管壁厚度占血管外径的百分比、管壁面积占血管总面积的百分比、增殖指数及p53基因表达较对照组均明显升高(P<0.01),管腔面积占血管总面积的百分比、凋亡指数较对照组明显降低(P<0.01);联合治疗组管壁面积占血管总面积的百分比、管壁厚度占血管外径的百分比、增殖指数及p53基因表达与低氧组相比明显降低,差别有统计学意义(P<0.01或P<0.05).管腔面积占血管总面积的百分比、凋亡指数与低氧组相比明显升高,差别有统计学意义(P<0.01);联合治疗组除管腔面积占血管总面积的百分比、凋亡指数及p53基因表达与对照组差异有统计学意义(P<0.01或P<0.05)外,其他指标与对照组比较差异均无统计学意义(P>0.05).结论阿魏酸钠可抑制慢性低氧大鼠肺血管重建,其机制可能与抑制p53及PCNA基因的表达,导致细胞增殖被抑制、细胞凋亡被促进等有关.
목적 연구아위산납대만성저양대서폐혈관형태、p53급PCNA단백표체급세포증식여조망적영향.방법 30지웅성SD대서채용수궤단위조설계방법분위대조조、저양조화연합치료조,매조10지.채용상압간단저양8h/d,련속21d적방법복제만성저양성폐동맥고압모형.대조조흡입공기(기타조건여저양조상동).연합치료조대서복강주사15 ms/(kg·d)적아위산납.관찰아위산납대만성저양대서폐혈관형태학지표、p53급증식세포핵항원PCNA기인단백표체、세포적증식여조망적영향.결과 저양21 d후,저양조관벽후도점혈관외경적백분비、관벽면적점혈관총면적적백분비、증식지수급p53기인표체교대조조균명현승고(P<0.01),관강면적점혈관총면적적백분비、조망지수교대조조명현강저(P<0.01);연합치료조관벽면적점혈관총면적적백분비、관벽후도점혈관외경적백분비、증식지수급p53기인표체여저양조상비명현강저,차별유통계학의의(P<0.01혹P<0.05).관강면적점혈관총면적적백분비、조망지수여저양조상비명현승고,차별유통계학의의(P<0.01);연합치료조제관강면적점혈관총면적적백분비、조망지수급p53기인표체여대조조차이유통계학의의(P<0.01혹P<0.05)외,기타지표여대조조비교차이균무통계학의의(P>0.05).결론 아위산납가억제만성저양대서폐혈관중건,기궤제가능여억제p53급PCNA기인적표체,도치세포증식피억제、세포조망피촉진등유관.
Objective To investigate the effect of sodium ferulate on vascular morphologic indexes of pul-monary,expression of p53 and PCNA, cell proliferation and apoptosis in chronic hypoxic rats. Methods Thirty healthy Spraque-Dawley male rats were randomly divided into three groups: normal control group (n = 10), chronic hypoxic group (n = 10) and hypoxia plus sodium ferulate treatment group (n = 10). The model of chronic hypoxic pulmonary hypertension was established by exposing rats to normobaric hypoxic conditions for 8 hours one day in 3 weeks continually. Effects of sodium ferulate on vascular morphologic indexes of pulmonary, expression of P53 and PCNA, cell proliferation and apoptosis in pulmonary tissue of HPH rats were observed. Results After 21 days, rats in chronic hypoxic group in MT%, MA%, PI, expression of p53 are obviously higher than normal control group (P < 0.01 ), in VA%, AI are distinctly higher than normal control group (P < 0.01);Rats of hypoxia plus sodium ferulate treatment group in MT%, MA%, PI and expression of p53 are obviously decreased than chronic hypoxic group (P < 0.01 or P < 0.05 ), in VA% and AI are increased higher than chronic hypoxic group (P < 0.01);Ex-cept that VA% ,AI and expression of p53 have meaning (P < 0.01 or P < 0.05 ), other indexes have no meaning compared with normal control group (P > 0.05). Conclusion Sodium ferulate can refrain persistently from the de-velopment of pulmonary vascular remodeling efficiently in chronic hypoxic rats. The mechanism may be related to in-hibiting the protein expression of p53 and PCNA, enhancing cell proliferation and decreasing cell apoptosis.