CAJ | 학술논문

目的研究β-连环蛋白(β-catenin)及细胞周期蛋白D1 (cyclin-D1)在散发性甲状旁腺瘤中的表达及其临床意义.方法应用免疫组化、荧光定量PCR( RT-PCR)检测20例散发性甲状旁腺瘤、10例甲状旁腺增生、8例正常甲状旁腺组织中β-catenin、cyclin-D1的表达.结果免疫组化显示:β-catenin 在正常甲状旁腺组织、甲状旁腺增生及散发性甲状旁腺瘤胞膜中的表达强度呈下降趋势,而在胞质和细胞核内逐渐增强；cyclin-D1在腺瘤组和增生组的表达明显高于正常组(P＜0.05),而腺瘤组与增生组比较差异无统计学意义(P＞0.05)；β-catenin异常表达与cyclin-D1的高表达在散发性甲状旁腺瘤中存在显著相关性(P＜0.05).荧光定量PCR分析显示:cyclin-D1 mRNA在腺瘤组织和正常甲状旁腙组织中的表达分别为2.36±1.12和1.50±1.03(P＜0.05),β-catenin mRNA在腺瘤组织和正常甲状旁腺组织中的表达分别为1.02±0.45和0.88±0.56(P ＞0.05).结论 β-catenin的异常表达激活cyclin-D1引起细胞增殖和分化失控,可能是散发性甲状旁腺瘤发生机制之一.
목적 연구β-련배단백(β-catenin)급세포주기단백D1 (cyclin-D1)재산발성갑상방선류중적표체급기림상의의.방법 응용면역조화、형광정량PCR( RT-PCR)검측20례산발성갑상방선류、10례갑상방선증생、8례정상갑상방선조직중β-catenin、cyclin-D1적표체.결과 면역조화현시:β-catenin 재정상갑상방선조직、갑상방선증생급산발성갑상방선류포막중적표체강도정하강추세,이재포질화세포핵내축점증강；cyclin-D1재선류조화증생조적표체명현고우정상조(P＜0.05),이선류조여증생조비교차이무통계학의의(P＞0.05)；β-catenin이상표체여cyclin-D1적고표체재산발성갑상방선류중존재현저상관성(P＜0.05).형광정량PCR분석현시:cyclin-D1 mRNA재선류조직화정상갑상방종조직중적표체분별위2.36±1.12화1.50±1.03(P＜0.05),β-catenin mRNA재선류조직화정상갑상방선조직중적표체분별위1.02±0.45화0.88±0.56(P ＞0.05).결론 β-catenin적이상표체격활cyclin-D1인기세포증식화분화실공,가능시산발성갑상방선류발생궤제지일.
Objective To study the expression of β-catenin and cyclin-D1 in sporadic parathyroid adenomas and the clinical significance.Methods Immunohistochemistry and RT-PCR were used to examine the expression of β-catenin and cyclin-Dl in 20 cases of sporadic parathyroid adenomas,10 cases of parathyroid hyperplasia tissues and 8 cases of normal parathyroid tissues respectively.Results The results of immunohistochemistry showed that the expression of β-catenin was decreased on cell membrane of normal parathyroid tissues,parathyroid hyperplasia tissues and sporadic parathyroid adenomas,and was increased in the cytoplasm and the nucleus.The expression of cyclin-Dl was significantly higher in the adenoma group and hyperplasia group than in the normal group( P ＜ 0.05 ).The difference had no statistical significance in terms of cyclin-Dl expression between the adenoma group and the hyperplasia group( P ＞0.05 ).The abnormal expression of β-catenin was significantly correlated with the overexpression of cyclin-D1 in sporadic parathyroid adenomas( P ＜ 0.05 ).RT-PCR analysis showed that the expression of cyclin-D1 mRNA was 2.36 ± 1.12 vs 1.50 ± 1.03 ( P ＜ 0.05 ),and the expression of β-catenin mRNA was 1.02 ± 0.45 vs 0.88 ± 0.56( P ＞ 0.05 ) in adenomas and normal parathyroid tissues respectively.Conclusion The abnormal expression of β-catenin activates cyclin-Dl and thus leads to the uncontrolled cell proliferation and differentiation,which may be one of the mechanisms of the occurrence of sporadic parathyroid tumors.