中国实用眼科杂志
中國實用眼科雜誌
중국실용안과잡지
CHINESE JOURNAL OF PRACTICAL OPHTHALMOLOGY
2011年
6期
620-622
,共3页
白内障晶状体上皮细胞%Bcl-2%Bax%免疫组织化学
白內障晶狀體上皮細胞%Bcl-2%Bax%免疫組織化學
백내장정상체상피세포%Bcl-2%Bax%면역조직화학
Lens epithelial cells of cataract%Bcl-2%Bax
目的 探讨随着年龄的增长白内障晶状体上皮细胞Bcl-2、Bax的基因表达与白内障发生的关系,及其在白内障发生发展中的作用机制.方法 选取行超声乳化白内障手术的患者91例91只眼,取晶状体前囊膜.根据年龄分为3组,即Ⅰ组为≤30岁的患者11例,Ⅱ组为31～60岁的患者30例,Ⅲ组为≥61岁的患者50例.采用链霉菌亲生物素蛋白-过氧化物酶标免疫组织化学法(streptavidin-peroxidase,SP)染色,镜下检测三个不同年龄组白内障患者晶状体上皮细胞Bcl-2基因蛋白与Bax基因蛋白的表达情况.结果 Ⅰ、Ⅱ、Ⅲ组Bcl-2基因表达分别为89%、60%、0.5%,Bax基因表达分别为0、11%、91%.各项指标经统计学分析具有统计学意义(P<0.05).结论 随着年龄的增长白内障晶状体上皮细胞Bcl-2基因表达逐渐降低,Bax基因表达逐渐增高,为在基因水平上治疗白内障提供了理论依据.
目的 探討隨著年齡的增長白內障晶狀體上皮細胞Bcl-2、Bax的基因錶達與白內障髮生的關繫,及其在白內障髮生髮展中的作用機製.方法 選取行超聲乳化白內障手術的患者91例91隻眼,取晶狀體前囊膜.根據年齡分為3組,即Ⅰ組為≤30歲的患者11例,Ⅱ組為31～60歲的患者30例,Ⅲ組為≥61歲的患者50例.採用鏈黴菌親生物素蛋白-過氧化物酶標免疫組織化學法(streptavidin-peroxidase,SP)染色,鏡下檢測三箇不同年齡組白內障患者晶狀體上皮細胞Bcl-2基因蛋白與Bax基因蛋白的錶達情況.結果 Ⅰ、Ⅱ、Ⅲ組Bcl-2基因錶達分彆為89%、60%、0.5%,Bax基因錶達分彆為0、11%、91%.各項指標經統計學分析具有統計學意義(P<0.05).結論 隨著年齡的增長白內障晶狀體上皮細胞Bcl-2基因錶達逐漸降低,Bax基因錶達逐漸增高,為在基因水平上治療白內障提供瞭理論依據.
목적 탐토수착년령적증장백내장정상체상피세포Bcl-2、Bax적기인표체여백내장발생적관계,급기재백내장발생발전중적작용궤제.방법 선취행초성유화백내장수술적환자91례91지안,취정상체전낭막.근거년령분위3조,즉Ⅰ조위≤30세적환자11례,Ⅱ조위31～60세적환자30례,Ⅲ조위≥61세적환자50례.채용련매균친생물소단백-과양화물매표면역조직화학법(streptavidin-peroxidase,SP)염색,경하검측삼개불동년령조백내장환자정상체상피세포Bcl-2기인단백여Bax기인단백적표체정황.결과 Ⅰ、Ⅱ、Ⅲ조Bcl-2기인표체분별위89%、60%、0.5%,Bax기인표체분별위0、11%、91%.각항지표경통계학분석구유통계학의의(P<0.05).결론 수착년령적증장백내장정상체상피세포Bcl-2기인표체축점강저,Bax기인표체축점증고,위재기인수평상치료백내장제공료이론의거.
Objective To evaluate the relationship between cataract lens epithelial cell and Bcl-2, Bax expression with increasing age in patients, and to investigate the role of Bcl-2 and Bax expression in the occurrence and progression of human lens epithelial cells of cataract.Methods Ninety-one patients (91 eyes) who were done phacoemulsification for cataract were selected and took anterior capsule of lens.The cataract patients were divided into three groups according to age: 11 cases of less than 30 years of age in group Ⅰ , 30 cases of aged 31-60 in group Ⅱ, 50 cases more than 61 years of age in group Ⅲ.The expression of Bcl-2 and Bax in lens epithelial cells of cataract from three different age groups of cataract patient was detected by the immunohistochemical streptavidin-peroxidase (SP) method.Results The Bcl-2 gene expression in group Ⅰ , Ⅱ, and Ⅲ was 89%, 60% and 0.5% respectively, the Bax gene expression was 0, 11% and 91% respectively.The difference had the statistical significance between 3 groups (P <0.05).Conclusions The genes expression of Bcl-2 in human lens epithelial cells of cataract is gradually decreasing with age increasing, while the genes expression of Bax is gradually increasing with age increasing.It provides an important theoretical basis to treat of cataract at the gene level.
